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Title: Thermostable cellulases, and mutants thereof, capable of hydrolyzing cellulose in ionic liquid

Abstract

The present invention provides for a composition comprising an ionic liquid and a thermostable cellulose, and a method of hydrolyzing a cellulose, comprising: (a) providing a composition comprising a solution comprising an ionic liquid and a cellulose, and (b) introducing a thermostable cellulase to the solution, such that the cellulose is hydrolyzed by the cellulase. The present invention also provides for a Thermatoga maritima thermostable cellulase mutant with increased cellulase activity.

Inventors:
; ; ; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1248892
Patent Number(s):
9,322,042
Application Number:
13/265,786
Assignee:
The Regents of the University of California (Oakland, CA) Sandia Corporation (Livermore, CA)
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2010 Apr 23
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 09 BIOMASS FUELS

Citation Formats

Sapra, Rajat, Datta, Supratim, Chen, Zhiwei, Holmes, Bradley M., Simmons, Blake A., and Blanch, Harvey W. Thermostable cellulases, and mutants thereof, capable of hydrolyzing cellulose in ionic liquid. United States: N. p., 2016. Web.
Sapra, Rajat, Datta, Supratim, Chen, Zhiwei, Holmes, Bradley M., Simmons, Blake A., & Blanch, Harvey W. Thermostable cellulases, and mutants thereof, capable of hydrolyzing cellulose in ionic liquid. United States.
Sapra, Rajat, Datta, Supratim, Chen, Zhiwei, Holmes, Bradley M., Simmons, Blake A., and Blanch, Harvey W. 2016. "Thermostable cellulases, and mutants thereof, capable of hydrolyzing cellulose in ionic liquid". United States. https://www.osti.gov/servlets/purl/1248892.
@article{osti_1248892,
title = {Thermostable cellulases, and mutants thereof, capable of hydrolyzing cellulose in ionic liquid},
author = {Sapra, Rajat and Datta, Supratim and Chen, Zhiwei and Holmes, Bradley M. and Simmons, Blake A. and Blanch, Harvey W.},
abstractNote = {The present invention provides for a composition comprising an ionic liquid and a thermostable cellulose, and a method of hydrolyzing a cellulose, comprising: (a) providing a composition comprising a solution comprising an ionic liquid and a cellulose, and (b) introducing a thermostable cellulase to the solution, such that the cellulose is hydrolyzed by the cellulase. The present invention also provides for a Thermatoga maritima thermostable cellulase mutant with increased cellulase activity.},
doi = {},
url = {https://www.osti.gov/biblio/1248892}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Apr 26 00:00:00 EDT 2016},
month = {Tue Apr 26 00:00:00 EDT 2016}
}

Works referenced in this record:

Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design
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Process for manufacture of imidazoles
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Methods for generating cellulases
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Biomass pretreatment
patent-application, September 2008