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Title: Energy optimization of a regular macromolecular crystallography beamline for ultra-high-resolution crystallography

Abstract

In this study, a practical method for operating existing undulator synchrotron beamlines at photon energies considerably higher than their standard operating range is described and applied at beamline 19-ID of the Structural Biology Center at the Advanced Photon Source enabling operation at 30 keV. Adjustments to the undulator spectrum were critical to enhance the 30 keV flux while reducing the lower- and higher-energy harmonic contamination. A Pd-coated mirror and Al attenuators acted as effective low- and high-bandpass filters. The resulting flux at 30 keV, although significantly lower than with X-ray optics designed and optimized for this energy, allowed for accurate data collection on crystals of the small protein crambin to 0.38 Å resolution.

Authors:
 [1];  [2];  [3]
  1. Argonne National Lab. (ANL), Argonne, IL (United States); The Univ. of Georgia, Athens, GA (United States)
  2. Argonne National Lab. (ANL), Argonne, IL (United States)
  3. Los Alamos National Lab. (LANL), Los Alamos, NM (United States); The Univ. of Toledo, Toledo, OH (United States)
Publication Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1248637
Report Number(s):
LA-UR-15-20476
Journal ID: ISSN 1600-5775; JSYRES; PII: S1600577514022619
Grant/Contract Number:
AC52-06NA25396
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Journal of Synchrotron Radiation (Online)
Additional Journal Information:
Journal Name: Journal of Synchrotron Radiation (Online); Journal Volume: 22; Journal Issue: 1; Journal ID: ISSN 1600-5775
Publisher:
International Union of Crystallography
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 47 OTHER INSTRUMENTATION; undulator; high energy; monochromator; harmonic

Citation Formats

Rosenbaum, Gerd, Ginell, Stephan L., and Chen, Julian C.-H. Energy optimization of a regular macromolecular crystallography beamline for ultra-high-resolution crystallography. United States: N. p., 2015. Web. doi:10.1107/S1600577514022619.
Rosenbaum, Gerd, Ginell, Stephan L., & Chen, Julian C.-H. Energy optimization of a regular macromolecular crystallography beamline for ultra-high-resolution crystallography. United States. doi:10.1107/S1600577514022619.
Rosenbaum, Gerd, Ginell, Stephan L., and Chen, Julian C.-H. Thu . "Energy optimization of a regular macromolecular crystallography beamline for ultra-high-resolution crystallography". United States. doi:10.1107/S1600577514022619. https://www.osti.gov/servlets/purl/1248637.
@article{osti_1248637,
title = {Energy optimization of a regular macromolecular crystallography beamline for ultra-high-resolution crystallography},
author = {Rosenbaum, Gerd and Ginell, Stephan L. and Chen, Julian C.-H.},
abstractNote = {In this study, a practical method for operating existing undulator synchrotron beamlines at photon energies considerably higher than their standard operating range is described and applied at beamline 19-ID of the Structural Biology Center at the Advanced Photon Source enabling operation at 30 keV. Adjustments to the undulator spectrum were critical to enhance the 30 keV flux while reducing the lower- and higher-energy harmonic contamination. A Pd-coated mirror and Al attenuators acted as effective low- and high-bandpass filters. The resulting flux at 30 keV, although significantly lower than with X-ray optics designed and optimized for this energy, allowed for accurate data collection on crystals of the small protein crambin to 0.38 Å resolution.},
doi = {10.1107/S1600577514022619},
journal = {Journal of Synchrotron Radiation (Online)},
number = 1,
volume = 22,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 2015},
month = {Thu Jan 01 00:00:00 EST 2015}
}

Journal Article:
Free Publicly Available Full Text
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Cited by: 3 works
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