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Title: Three dimensional time-gated tracking of non-blinking quantum dots in live cells

Abstract

Single particle tracking has provided a wealth of information about biophysical processes such as motor protein transport and diffusion in cell membranes. However, motion out of the plane of the microscope or blinking of the fluorescent probe used as a label generally limits observation times to several seconds. Here, we overcome these limitations by using novel non-blinking quantum dots as probes and employing a custom 3D tracking microscope to actively follow motion in three dimensions (3D) in live cells. As a result, signal-to-noise is improved in the cellular milieu through the use of pulsed excitation and time-gated detection.

Authors:
 [1];  [1];  [1];  [1];  [1];  [2];  [2];  [2];  [1];  [3];  [1];  [1]
  1. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
  2. Univ. of New Mexico, Albuquerque, NM (United States)
  3. U.S. Naval Research Lab., Washington, D.C. (United States)
Publication Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1247683
Report Number(s):
LA-UR-15-20205
Journal ID: ISSN 0277-786X
Grant/Contract Number:  
AC52-06NA25396
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Proceedings of SPIE - The International Society for Optical Engineering
Additional Journal Information:
Journal Volume: 9338; Journal ID: ISSN 0277-786X
Publisher:
SPIE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 77 NANOSCIENCE AND NANOTECHNOLOGY; Biological Science

Citation Formats

DeVore, Matthew S., Werner, James H., Goodwin, Peter M., Keller, Aaron M., Hollingsworth, Jennifer A., Wilson, Bridget S., Cleyrat, Cedric, Lidke, Diane S., Ghosh, Yagnaseni, Stewart, Michael H., Stich, Dominik G., and Phipps, Mary E. Three dimensional time-gated tracking of non-blinking quantum dots in live cells. United States: N. p., 2015. Web. doi:10.1117/12.2082943.
DeVore, Matthew S., Werner, James H., Goodwin, Peter M., Keller, Aaron M., Hollingsworth, Jennifer A., Wilson, Bridget S., Cleyrat, Cedric, Lidke, Diane S., Ghosh, Yagnaseni, Stewart, Michael H., Stich, Dominik G., & Phipps, Mary E. Three dimensional time-gated tracking of non-blinking quantum dots in live cells. United States. doi:10.1117/12.2082943.
DeVore, Matthew S., Werner, James H., Goodwin, Peter M., Keller, Aaron M., Hollingsworth, Jennifer A., Wilson, Bridget S., Cleyrat, Cedric, Lidke, Diane S., Ghosh, Yagnaseni, Stewart, Michael H., Stich, Dominik G., and Phipps, Mary E. Thu . "Three dimensional time-gated tracking of non-blinking quantum dots in live cells". United States. doi:10.1117/12.2082943. https://www.osti.gov/servlets/purl/1247683.
@article{osti_1247683,
title = {Three dimensional time-gated tracking of non-blinking quantum dots in live cells},
author = {DeVore, Matthew S. and Werner, James H. and Goodwin, Peter M. and Keller, Aaron M. and Hollingsworth, Jennifer A. and Wilson, Bridget S. and Cleyrat, Cedric and Lidke, Diane S. and Ghosh, Yagnaseni and Stewart, Michael H. and Stich, Dominik G. and Phipps, Mary E.},
abstractNote = {Single particle tracking has provided a wealth of information about biophysical processes such as motor protein transport and diffusion in cell membranes. However, motion out of the plane of the microscope or blinking of the fluorescent probe used as a label generally limits observation times to several seconds. Here, we overcome these limitations by using novel non-blinking quantum dots as probes and employing a custom 3D tracking microscope to actively follow motion in three dimensions (3D) in live cells. As a result, signal-to-noise is improved in the cellular milieu through the use of pulsed excitation and time-gated detection.},
doi = {10.1117/12.2082943},
journal = {Proceedings of SPIE - The International Society for Optical Engineering},
number = ,
volume = 9338,
place = {United States},
year = {Thu Mar 12 00:00:00 EDT 2015},
month = {Thu Mar 12 00:00:00 EDT 2015}
}

Journal Article:
Free Publicly Available Full Text
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Cited by: 2 works
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