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Title: Nanolipoprotein particles comprising a natural rubber biosynthetic enzyme complex and related products, methods and systems

Abstract

Provided herein are nanolipoprotein particles that comprise a biosynthetic enzyme more particularly an enzyme capable of catalyzing rubber or other rubbers polymerization, and related assemblies, devices, methods and systems.

Inventors:
;
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1245592
Patent Number(s):
9,303,273
Application Number:
14/199,973
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA) The United States of America, as Represented by the Secretary of Agriculture (Washington, DC)
DOE Contract Number:  
AC52-07NA27344
Resource Type:
Patent
Resource Relation:
Patent File Date: 2014 Mar 06
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 77 NANOSCIENCE AND NANOTECHNOLOGY

Citation Formats

Hoeprich, Paul D., and Whalen, Maureen. Nanolipoprotein particles comprising a natural rubber biosynthetic enzyme complex and related products, methods and systems. United States: N. p., 2016. Web.
Hoeprich, Paul D., & Whalen, Maureen. Nanolipoprotein particles comprising a natural rubber biosynthetic enzyme complex and related products, methods and systems. United States.
Hoeprich, Paul D., and Whalen, Maureen. 2016. "Nanolipoprotein particles comprising a natural rubber biosynthetic enzyme complex and related products, methods and systems". United States. https://www.osti.gov/servlets/purl/1245592.
@article{osti_1245592,
title = {Nanolipoprotein particles comprising a natural rubber biosynthetic enzyme complex and related products, methods and systems},
author = {Hoeprich, Paul D. and Whalen, Maureen},
abstractNote = {Provided herein are nanolipoprotein particles that comprise a biosynthetic enzyme more particularly an enzyme capable of catalyzing rubber or other rubbers polymerization, and related assemblies, devices, methods and systems.},
doi = {},
url = {https://www.osti.gov/biblio/1245592}, journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {4}
}

Works referenced in this record:

Rubber Elongation Factor (REF), a Major Allergen Component in Hevea brasiliensis Latex Has Amyloid Properties
journal, October 2012


Protein tolerance to random amino acid change
journal, June 2004


Transforming growth factor alpha: mutation of aspartic acid 47 and leucine 48 results in different biological activities.
journal, March 1988


Functional Analysis of Conserved Histidines in ADP-Glucose Pyrophosphorylase from Escherichia coli
journal, March 1998


Disentangling the perturbational effects of amino acid substitutions in the DNA-binding domain of p53
journal, February 1999


Protein engineering methods applied to membrane protein targets
journal, October 2012


Complex of Human Apolipoprotein C-1 with Phospholipid:  Thermodynamic or Kinetic Stability?
journal, June 2002


Pharmaceutical analysis of synthetic lipid A-based vaccine adjuvants in poly (d,l-lactic-co-glycolic acid) nanoparticle formulations
journal, August 2007


The growing impact of click chemistry on drug discovery
journal, December 2003


Energy-Converting [NiFe] Hydrogenases from Archaea and Extremophiles: Ancestors of Complex I
journal, February 2004


Click Chemistry, A Powerful Tool for Pharmaceutical Sciences
journal, May 2008


Enzyme-catalyzed Synthesis and Degradation of Biopolymers
journal, February 2009


Fabrication of biomolecule–copolymer hybrid nanovesicles as energy conversion systems
journal, November 2005


Induction of tumor necrosis factor-α in solid tumor region by the orally administered synthetic muramyl dipeptide analogue, romurtide
journal, January 2001


Structural Basis for Thermal Stability of Human Low-Density Lipoprotein
journal, March 2005


Indium-111 Labeling of Low Density Lipoproteins with the DTPA−Bis(stearylamide):  Evaluation as a Potential Radiopharmaceutical for Tumor Localization
journal, January 1996


Thermotolerant Hydrogenases: Biological Diversity, Properties, and Biotechnological Applications
journal, January 2008


Functional Cell-free Synthesis of a Seven Helix Membrane Protein: In situ Insertion of Bacteriorhodopsin into Liposomes
journal, August 2007


Insertion of Membrane Proteins into Discoidal Membranes Using a Cell-Free Protein Expression Approach
journal, August 2008


Gold Nanoparticle-Enhanced Secondary Ion Mass Spectrometry Imaging of Peptides on Self-Assembled Monolayers
journal, March 2006


High level cell-free expression and specific labeling of integral membrane proteins
journal, February 2004


Cell-free expression as an emerging technique for the large scale production of integral membrane protein
journal, September 2006


A novel approach for biohydrogen production
journal, September 2006


Functional reconstitution of β 2 -adrenergic receptors utilizing self-assembling Nanodisc technology
journal, May 2006


Conformational Reorganization of the Four-helix Bundle of Human Apolipoprotein E in Binding to Phospholipid
journal, May 2000


ARB: a software environment for sequence data
journal, February 2004


RNA Stable Isotope Probing, a Novel Means of Linking Microbial Community Function to Phylogeny
journal, November 2002


Large-scale profiling of protein palmitoylation in mammalian cells
journal, January 2009


The Efficiency of Light-Directed Synthesis of DNA Arrays on Glass Substrates
journal, June 1997

  • McGall, Glenn H.; Barone, Anthony D.; Diggelmann, Martin
  • Journal of the American Chemical Society, Vol. 119, Issue 22, p. 5081-5090
  • https://doi.org/10.1021/ja964427a

[FeFe] hydrogenases and their evolution: a genomic perspective
journal, March 2007


Functional Characterization of Apolipoprotein E Isoforms Overexpressed in Escherichia coli
journal, July 1999


Detection of DNA hybridization on indium tin oxide surfaces
journal, August 2007


Applications of Phospholipid Bilayer Nanodiscs in the Study of Membranes and Membrane Proteins
journal, February 2007


Immobilization of His-Tagged Proteins on Nickel-Chelating Nanolipoprotein Particles
journal, March 2009


The Difference a Se Makes? Oxygen-Tolerant Hydrogen Production by the [NiFeSe]-Hydrogenase from Desulfomicrobium baculatum
journal, October 2008


In-depth analysis of the membrane and cytosolic proteome of red blood cells
journal, August 2006


Topology prediction of membrane proteins
journal, February 1996


The Biosynthesis of Natural Rubber
journal, June 1990


Transcriptome and gene expression analysis in cold-acclimated guayule (Parthenium argentatum) rubber-producing tissue
journal, July 2012


Stable-isotope probing as a tool in microbial ecology
journal, February 2000


Recombinant lipoproteins: lipoprotein-like lipid particles for drug targeting
journal, April 2001


Comparison of Multiplexed Techniques for Detection of Bacterial and Viral Proteins
journal, August 2004


Chemistry: The photon trap
journal, March 2008


A simple energy-conserving system: Proton reduction coupled to proton translocation
journal, June 2003


Purification and Characterization of a Membrane-Bound Hydrogenase from the Hyperthermophilic Archaeon Pyrococcus furiosus
journal, June 2000


Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray
journal, October 1995


Characterization of rubber particles and rubber chain elongation in Taraxacum koksaghyz
journal, January 2010


Synthesis and Characterization of Chelator-Lipids for Reversible Immobilization of Engineered Proteins at Self-Assembled Lipid Interfaces
journal, September 1994


Laboratory scale structural genomics
journal, March 2004


The micromorphology and protein characterization of rubber particles in Ficus carica, Ficus benghalensis and Hevea brasiliensis
journal, March 2003


Maskless fabrication of light-directed oligonucleotide microarrays using a digital micromirror array
journal, October 1999


Membrane-Mimetic Films of Asymmetric Phosphatidylcholine Lipid Bolaamphiphiles
journal, January 2006


Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems
journal, January 2003


Peptide stabilized amphotericin B nanodisks
journal, April 2007


DNA-based stable isotope probing: a link between community structure and function
journal, June 2009


Occurrence, Classification, and Biological Function of Hydrogenases:  An Overview
journal, October 2007


From The Cover: Electrocatalytic hydrogen oxidation by an enzyme at high carbon monoxide or oxygen levels
journal, October 2005


Investigating and Exploiting the Electrocatalytic Properties of Hydrogenases
journal, October 2007


Comparison of the Dynamics of the Primary Events of Bacteriorhodopsin in Its Trimeric and Monomeric States
journal, September 2002


CpG DNA induces stronger immune responses with less toxicity than other adjuvants
journal, March 2000


In vitro hydrogen production by glucose dehydrogenase and hydrogenase
journal, July 1996


Enzymatic production of biohydrogen: Biotechnology
journal, June 2000


High yield cell-free production of integral membrane proteins without refolding or detergents
journal, May 2008


Initiation of rubber biosynthesis: In vitro comparisons of benzophenone-modified diphosphate analogues in three rubber-producing species
journal, October 2008


High-Yield Hydrogen Production from Starch and Water by a Synthetic Enzymatic Pathway
journal, May 2007


Model of Biologically Active Apolipoprotein E Bound to Dipalmitoylphosphatidylcholine
journal, November 2005


A monomeric G protein-coupled receptor isolated in a high-density lipoprotein particle efficiently activates its G protein
journal, April 2007


In Vitro Reconstitution of Assembly of Apolipoprotein B48-containing Lipoproteins
journal, March 1997


Homotropic cooperativity of monomeric cytochrome P450 3A4 in a nanoscale native bilayer environment
journal, October 2004


Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB
journal, July 2006


Conjugation to Nickel-Chelating Nanolipoprotein Particles Increases the Potency and Efficacy of Subunit Vaccines to Prevent West Nile Encephalitis
journal, June 2010


Indirect electrochemical reduction of nicotinamide coenzymes
journal, December 2004


Kinetic properties of recombinant MAO-A on incorporation into phospholipid nanodisks
journal, March 2007


Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles
journal, February 2010

  • Sperling, R. A.; Parak, W. J.
  • Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, Vol. 368, Issue 1915, p. 1333-1383
  • https://doi.org/10.1098/rsta.2009.0273

Synthesis of Native Proteins by Chemical Ligation
journal, June 2000


A bilayer cell-free protein synthesis system for high-throughput screening of gene products
journal, February 2002


Expression of G protein coupled receptors in a cell-free translational system using detergents and thioredoxin-fusion vectors
journal, May 2005


Single-molecule height measurements on microsomal cytochrome P450 in nanometer-scale phospholipid bilayer disks
journal, May 2002


Different Apolipoproteins Impact Nanolipoprotein Particle Formation
journal, November 2007

  • Chromy, Brett A.; Arroyo, Erin; Blanchette, Craig D.
  • Journal of the American Chemical Society, Vol. 129, Issue 46, p. 14348-14354
  • https://doi.org/10.1021/ja074753y

Phospholipid phase transitions in homogeneous nanometer scale bilayer discs
journal, December 2003


Self-Assembly of Discoidal Phospholipid Bilayer Nanoparticles with Membrane Scaffold Proteins
journal, August 2002


Atomic Force Microscope Studies of the Fusion of Floating Lipid Bilayers
journal, June 2007


Stable isotope probing: Technical considerations when resolving 15N-labeled RNA in gradients
journal, January 2010


Hydrogen Production by a Hyperthermophilic Membrane-Bound Hydrogenase in Water-Soluble Nanolipoprotein Particles
journal, June 2009


High-Sensitivity Detection of DNA Hybridization on Microarrays Using Resonance Light Scattering
journal, April 2002


Reconstitution and Imaging of a Membrane Protein in a Nanometer-Size Phospholipid Bilayer
journal, September 1998


Self-assembly of single integral membrane proteins into soluble nanoscale phospholipid bilayers
journal, November 2003


Assembly of single bacteriorhodopsin trimers in bilayer nanodiscs
journal, June 2006


Specific targeting of a lipophilic prodrug of iododeoxyuridine to parenchymal liver cells using lactosylated reconstituted high density lipoprotein particles
journal, July 1996


Quantifying size distributions of nanolipoprotein particles with single-particle analysis and molecular dynamic simulations
journal, April 2008


Atomic force microscopy differentiates discrete size distributions between membrane protein containing and empty nanolipoprotein particles
journal, March 2009


Do Recombinant Receptor Assays Provide Affinity and Provide Estimates?
journal, May 1997


Nanodiscs separate chemoreceptor oligomeric states and reveal their signaling properties
journal, July 2006


Nanodiscs for Immobilization of Lipid Bilayers and Membrane Receptors: Kinetic Analysis of Cholera Toxin Binding to a Glycolipid Receptor
journal, August 2008


Direct linking of microbial populations to specific biogeochemical processes by 13C-labelling of biomarkers
journal, April 1998


Formation of Thiolate and Phosphonate Adlayers on Indium−Tin Oxide:  Optical and Electronic Characterization
journal, September 2002


Application of a High-Density Oligonucleotide Microarray Approach To Study Bacterial Population Dynamics during Uranium Reduction and Reoxidation
journal, September 2006


Urban aerosols harbor diverse and dynamic bacterial populations
journal, December 2006


Exploring the new world of the genome with DNA microarrays
journal, January 1999


Chemoselective Attachment of Biologically Active Proteins to Surfaces by Expressed Protein Ligation and Its Application for “Protein Chip” Fabrication
journal, November 2004

  • Camarero, Julio A.; Kwon, Youngeun; Coleman, Matthew A.
  • Journal of the American Chemical Society, Vol. 126, Issue 45, p. 14730-14731
  • https://doi.org/10.1021/ja0456611

Cell-free Co-expression of Functional Membrane Proteins and Apolipoprotein, Forming Soluble Nanolipoprotein Particles
journal, July 2008


Protein Prenyltransferases
journal, March 1996


Integration of biological networks and gene expression data using Cytoscape
journal, September 2007


Asp 46 can substitute for Asp 96 as the Schiff base proton donor in bacteriorhodopsin
journal, November 1995


An improved synthesis of isopentenyl pyrophosphate
journal, November 1967


Immobilization of Active Hydrogenases by Encapsulation in Polymeric Porous Gels
journal, October 2005


IMMUNOLOGY: The Shape of Things to Come
journal, June 2007


Carboxyl-Terminal Domain of Human Apolipoprotein E: Expression, Purification, and Crystallization
journal, November 1999


Electron microscopic study on reassembly of plasma high density apoprotein with various lipids
journal, November 1971


Systems for orthogonal self-assembly of electroactive monolayers on Au and ITO: an approach to molecular electronics
journal, July 1995


Hydrogen Production under Aerobic Conditions by Membrane-Bound Hydrogenases from Ralstonia Species
journal, August 2008