Advancing understanding of microbial bioenergy conversion processes by activity-based protein profiling

Liu, Yun; Fredrickson, James K.; Sadler, Natalie C.; Nandhikonda, Premchendar; Smith, Richard D.; Wright, Aaron T.

doi:10.1186/s13068-015-0343-7

Title: Advancing understanding of microbial bioenergy conversion processes by activity-based protein profiling

Journal Article · Fri Sep 25 00:00:00 EDT 2015 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-015-0343-7· OSTI ID:1243254

Liu, Yun ^[1]; Fredrickson, James K. ^[2]; Sadler, Natalie C. ^[2]; Nandhikonda, Premchendar ^[2]; Smith, Richard D. ^[2]; Wright, Aaron T. ^[2]

Beijing Univ. of Chemical Technology, Beijing (China)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Here, the development of renewable biofuels is a global priority, but success will require novel technologies that greatly improve our understanding of microbial systems biology. An approach with great promise in enabling functional characterization of microbes is activity-based protein profiling (ABPP), which employs chemical probes to directly measure enzyme function in discrete enzyme classes in vivo and/or in vitro, thereby facilitating the rapid discovery of new biocatalysts and enabling much improved biofuel production platforms. We review general design strategies in ABPP, and highlight recent advances that are or could be pivotal to biofuels processes including applications of ABPP to cellulosic bioethanol, biodiesel, and phototrophic production of hydrocarbons. We also examine the key challenges and opportunities of ABPP in renewable biofuels research. The integration of ABPP with molecular and systems biology approaches will shed new insight on the catalytic and regulatory mechanisms of functional enzymes and their synergistic effects in the field of biofuels production.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1243254

Report Number(s):: PNNL-SA-107650; 48680; 48672; KP1601010

Journal Information:: Biotechnology for Biofuels, Vol. 8; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 16 works

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References (80)

The Path Forward for Biofuels and Biomaterials Ragauskas, Arthur J.; Williams, Charlotte K.; Davison, Brian H. Science, Vol. 311, Issue 5760, p. 484-489 https://doi.org/10.1126/science.1114736	journal	January 2006
Genomics of cellulosic biofuels Rubin, Edward M. Nature, Vol. 454, Issue 7206 https://doi.org/10.1038/nature07190	journal	August 2008
Insights into the phylogeny and coding potential of microbial dark matter Rinke, Christian; Schwientek, Patrick; Sczyrba, Alexander Nature, Vol. 499, Issue 7459 https://doi.org/10.1038/nature12352	journal	July 2013
Activity-based protein profiling of microbes Sadler, Natalie C.; Wright, Aaron T. Current Opinion in Chemical Biology, Vol. 24 https://doi.org/10.1016/j.cbpa.2014.10.022	journal	February 2015
Current Developments in Activity-Based Protein Profiling Willems, Lianne I.; Overkleeft, Herman S.; van Kasteren, Sander I. Bioconjugate Chemistry, Vol. 25, Issue 7 https://doi.org/10.1021/bc500208y	journal	June 2014
Activity-Based Protein Profiling: From Enzyme Chemistry to Proteomic Chemistry Cravatt, Benjamin F.; Wright, Aaron T.; Kozarich, John W. Annual Review of Biochemistry, Vol. 77, Issue 1 https://doi.org/10.1146/annurev.biochem.75.101304.124125	journal	June 2008
Chemical ‘omics’ approaches for understanding protein cysteine oxidation in biology Leonard, Stephen E.; Carroll, Kate S. Current Opinion in Chemical Biology, Vol. 15, Issue 1 https://doi.org/10.1016/j.cbpa.2010.11.012	journal	February 2011
Small-molecule probes elucidate global enzyme activity in a proteomic context Lee, Jun-Seok; Yoo, Young-Hwa; Yoon, Chang No BMB Reports, Vol. 47, Issue 3 https://doi.org/10.5483/BMBRep.2014.47.3.264	journal	March 2014
Mapping sites of aspirin-induced acetylations in live cells by quantitative acid-cleavable activity-based protein profiling (QA-ABPP) Wang, Jigang; Zhang, Chong-Jing; Zhang, Jianbin Scientific Reports, Vol. 5, Issue 1 https://doi.org/10.1038/srep07896	journal	January 2015
Activity-based proteomic and metabolomic approaches for understanding metabolism Hunerdosse, Devon; Nomura, Daniel K. Current Opinion in Biotechnology, Vol. 28 https://doi.org/10.1016/j.copbio.2014.02.001	journal	August 2014
Activity-based protein profiling for biochemical pathway discovery in cancer Nomura, Daniel K.; Dix, Melissa M.; Cravatt, Benjamin F. Nature Reviews Cancer, Vol. 10, Issue 9 https://doi.org/10.1038/nrc2901	journal	August 2010
Pretubulysin derived probes as novel tools for monitoring the microtubule network via activity-based protein profiling and fluorescence microscopy Eirich, Jürgen; Burkhart, Jens L.; Ullrich, Angelika Molecular BioSystems, Vol. 8, Issue 8 https://doi.org/10.1039/c2mb25144b	journal	January 2012
Organelle-Specific Activity-Based Protein Profiling in Living Cells Wiedner, Susan D.; Anderson, Lindsey N.; Sadler, Natalie C. Angewandte Chemie International Edition, Vol. 53, Issue 11 https://doi.org/10.1002/anie.201309135	journal	February 2014
Functional Imaging of Legumain in Cancer Using a New Quenched Activity-Based Probe Edgington, Laura E.; Verdoes, Martijn; Ortega, Alberto Journal of the American Chemical Society, Vol. 135, Issue 1 https://doi.org/10.1021/ja307083b	journal	December 2012
Activity-based protein profiling: The serine hydrolases Liu, Y.; Patricelli, M. P.; Cravatt, B. F. Proceedings of the National Academy of Sciences, Vol. 96, Issue 26 https://doi.org/10.1073/pnas.96.26.14694	journal	December 1999
Activity-Based Profiling of Proteases Sanman, Laura E.; Bogyo, Matthew Annual Review of Biochemistry, Vol. 83, Issue 1 https://doi.org/10.1146/annurev-biochem-060713-035352	journal	June 2014
Activity-Based Probes for the Study of Proteases: Recent Advances and Developments Serim, Sevnur; Haedke, Ute; Verhelst, Steven H. L. ChemMedChem, Vol. 7, Issue 7 https://doi.org/10.1002/cmdc.201200057	journal	March 2012
Activity-based probes for the proteomic profiling of metalloproteases Saghatelian, A.; Jessani, N.; Joseph, A. Proceedings of the National Academy of Sciences, Vol. 101, Issue 27 https://doi.org/10.1073/pnas.0402784101	journal	June 2004
Functional Interrogation of the Kinome Using Nucleotide Acyl Phosphates Patricelli, Matthew P.; Szardenings, A. Katrin; Liyanage, Marek Biochemistry, Vol. 46, Issue 2 https://doi.org/10.1021/bi062142x	journal	January 2007
Activity-Based Protein Profiling Reveals Mitochondrial Oxidative Enzyme Impairment and Restoration in Diet-Induced Obese Mice Sadler, Natalie C.; Angel, Thomas E.; Lewis, Michael P. PLoS ONE, Vol. 7, Issue 10 https://doi.org/10.1371/journal.pone.0047996	journal	October 2012
Chemical Proteomic Probes for Profiling Cytochrome P450 Activities and Drug Interactions In Vivo Wright, Aaron T.; Cravatt, Benjamin F. Chemistry & Biology, Vol. 14, Issue 9 https://doi.org/10.1016/j.chembiol.2007.08.008	journal	September 2007
Activity-based probes for proteomic profiling of histone deacetylase complexes Salisbury, C. M.; Cravatt, B. F. Proceedings of the National Academy of Sciences, Vol. 104, Issue 4 https://doi.org/10.1073/pnas.0608659104	journal	January 2007
Optimization of Activity-Based Probes for Proteomic Profiling of Histone Deacetylase Complexes Salisbury, Cleo M.; Cravatt, Benjamin F. Journal of the American Chemical Society, Vol. 130, Issue 7 https://doi.org/10.1021/ja074138u	journal	February 2008
An Inhibitor of Glutathione S-Transferase Omega 1 that Selectively Targets Apoptotic Cells Pace, Nicholas J.; Pimental, Daniel R.; Weerapana, Eranthie Angewandte Chemie International Edition, Vol. 51, Issue 33 https://doi.org/10.1002/anie.201203730	journal	July 2012
Active-site Peptide “Fingerprinting” of Glycosidases in Complex Mixtures by Mass Spectrometry Hekmat, Omid; Kim, Young-Wan; Williams, Spencer J. Journal of Biological Chemistry, Vol. 280, Issue 42 https://doi.org/10.1074/jbc.M508434200	journal	August 2005
A Mechanism-Based ICAT Strategy for Comparing Relative Expression and Activity Levels of Glycosidases in Biological Systems Hekmat, Omid; He, Shouming; Warren, R. Antony J. Journal of Proteome Research, Vol. 7, Issue 8 https://doi.org/10.1021/pr7008302	journal	August 2008
Suite of Activity-Based Probes for Cellulose-Degrading Enzymes Chauvigné-Hines, Lacie M.; Anderson, Lindsey N.; Weaver, Holly M. Journal of the American Chemical Society, Vol. 134, Issue 50 https://doi.org/10.1021/ja309790w	journal	December 2012
Activity-Based Profiling of Retaining β-Glucosidases: A Comparative Study Witte, Martin D.; Walvoort, Marthe T. C.; Li, Kah-Yee ChemBioChem, Vol. 12, Issue 8 https://doi.org/10.1002/cbic.201000773	journal	April 2011
Building better bioorthogonal reactions Shih, Hui-Wen; Kamber, David N.; Prescher, Jennifer A. Current Opinion in Chemical Biology, Vol. 21 https://doi.org/10.1016/j.cbpa.2014.07.002	journal	August 2014
Applications of Copper-Catalyzed Click Chemistry in Activity-Based Protein Profiling Martell, Julianne; Weerapana, Eranthie Molecules, Vol. 19, Issue 2 https://doi.org/10.3390/molecules19021378	journal	January 2014
The carbohydrate-active enzymes database (CAZy) in 2013 Lombard, Vincent; Golaconda Ramulu, Hemalatha; Drula, Elodie Nucleic Acids Research, Vol. 42, Issue D1 https://doi.org/10.1093/nar/gkt1178	journal	November 2013
Genomics of wood-degrading fungi Ohm, Robin A.; Riley, Robert; Salamov, Asaf Fungal Genetics and Biology, Vol. 72 https://doi.org/10.1016/j.fgb.2014.05.001	journal	November 2014
A review of enzymes and microbes for lignocellulosic biorefinery and the possibility of their application to consolidated bioprocessing technology Hasunuma, Tomohisa; Okazaki, Fumiyoshi; Okai, Naoko Bioresource Technology, Vol. 135 https://doi.org/10.1016/j.biortech.2012.10.047	journal	May 2013
Activity-based proteomics probes for carbohydrate-processing enzymes: current trends and future outlook Stubbs, Keith A. Carbohydrate Research, Vol. 390 https://doi.org/10.1016/j.carres.2014.02.023	journal	May 2014
Activity-based protein profiling of secreted cellulolytic enzyme activity dynamics in Trichoderma reesei QM6a, NG14, and RUT-C30 Anderson, Lindsey N.; Culley, David E.; Hofstad, Beth A. Molecular BioSystems, Vol. 9, Issue 12 https://doi.org/10.1039/c3mb70333a	journal	January 2013
Investigation of the active site of Escherichia coli β-d-galactosidase by photoaffinity labelling Kuhn, Cai-Steffen; Lehmann, Jochen; Jung, Günther Carbohydrate Research, Vol. 232, Issue 2 https://doi.org/10.1016/0008-6215(92)80056-7	journal	August 1992
A Strategy for Functional Proteomic Analysis of Glycosidase Activity from Cell Lysates Vocadlo, David J.; Bertozzi, Carolyn R. Angewandte Chemie International Edition, Vol. 43, Issue 40 https://doi.org/10.1002/anie.200454235	journal	October 2004
Synthesis of a novel photoaffinity derivative of 1-deoxynojirimycin for active site-directed labeling of glucosidase I Romaniouk, A. V. Glycobiology, Vol. 14, Issue 4 https://doi.org/10.1093/glycob/cwh044	journal	January 2004
Synthesis and Testing of Mechanism-Based Protein-Profiling Probes for Retaining Endo-glycosidases Williams, Spencer J.; Hekmat, Omid; Withers, Stephen G. ChemBioChem, Vol. 7, Issue 1 https://doi.org/10.1002/cbic.200500279	journal	January 2006
Fluorescently labeled inhibitor for profiling cytoplasmic peptide:N-glycanase Hagihara, Shinya; Miyazaki, Ayako; Matsuo, Ichiro Glycobiology, Vol. 17, Issue 10 https://doi.org/10.1093/glycob/cwm079	journal	July 2007
Synthesis and Use of Mechanism-Based Protein-Profiling Probes for Retaining β- d -Glucosaminidases Facilitate Identification of Pseudomonas aeruginosa NagZ Stubbs, Keith A.; Scaffidi, Adrian; Debowski, Aleksandra W. Journal of the American Chemical Society, Vol. 130, Issue 1 https://doi.org/10.1021/ja0763605	journal	January 2008
A general method for affinity-based proteomic profiling of exo-α-glycosidases Gandy, Michael N.; Debowski, Aleksandra W.; Stubbs, Keith A. Chemical Communications, Vol. 47, Issue 17 https://doi.org/10.1039/c1cc10308c	journal	January 2011
A mechanism-based inactivator of glycoside hydrolases involving formation of a transient non-classical carbocation Chakladar, Saswati; Wang, Yi; Clark, Thomas Nature Communications, Vol. 5, Issue 1 https://doi.org/10.1038/ncomms6590	journal	December 2014
Novozym 435-catalyzed synthesis of fatty acid ethyl esters from soybean oil for biodiesel production Cerveró, J. M.; Álvarez, J. R.; Luque, S. Biomass and Bioenergy, Vol. 61 https://doi.org/10.1016/j.biombioe.2013.12.005	journal	February 2014
Biodiesel synthesis combining pre-esterification with alkali catalyzed process from rapeseed oil deodorizer distillate Liu, Yun; Wang, Ling; Yan, Yunjun Fuel Processing Technology, Vol. 90, Issue 7-8 https://doi.org/10.1016/j.fuproc.2009.04.005	journal	July 2009
Microbial biodiesel production by direct methanolysis of oleaginous biomass Thliveros, Panagiotis; Uçkun Kiran, Esra; Webb, Colin Bioresource Technology, Vol. 157 https://doi.org/10.1016/j.biortech.2014.01.111	journal	April 2014
Study of the microwave lipid extraction from microalgae for biodiesel production Dai, Yong-Ming; Chen, Kung-Tung; Chen, Chiing-Chang Chemical Engineering Journal, Vol. 250 https://doi.org/10.1016/j.cej.2014.04.031	journal	August 2014
Biodiesel as feasible petrol fuel replacement: a multidisciplinary overview Luque, Rafael; Lovett, Jon C.; Datta, Bipasa Energy & Environmental Science, Vol. 3, Issue 11 https://doi.org/10.1039/c0ee00085j	journal	January 2010
Lipase-catalyzed process for biodiesel production: Protein engineering and lipase production: Lipase-Catalyzed Process for Biodiesel Production Hwang, Hyun Tae; Qi, Feng; Yuan, Chongli Biotechnology and Bioengineering, Vol. 111, Issue 4 https://doi.org/10.1002/bit.25162	journal	December 2013
Techniques to measure lipase and esterase activity in vitro Gilham, Dean; Lehner, Richard Methods, Vol. 36, Issue 2 https://doi.org/10.1016/j.ymeth.2004.11.003	journal	June 2005
An activity-based probe for high-throughput measurements of triacylglycerol lipases Tam, John; Henault, Martin; Li, Lianhai Analytical Biochemistry, Vol. 414, Issue 2 https://doi.org/10.1016/j.ab.2011.03.008	journal	July 2011
A versatile library of activity-based probes for fluorescence detection and/or affinity isolation of lipolytic enzymes Susani-Etzerodt, Heidrun; Schmidinger, Hannes; Riesenhuber, Gernot Chemistry and Physics of Lipids, Vol. 144, Issue 1 https://doi.org/10.1016/j.chemphyslip.2006.06.018	journal	October 2006
Lipolytic and esterolytic activity-based profiling of murine liver Birner-Gruenberger, Ruth; Susani-Etzerodt, Heidrun; Kollroser, Manfred PROTEOMICS, Vol. 8, Issue 17 https://doi.org/10.1002/pmic.200800191	journal	September 2008
Activity-Based Proteomics of Lipolytic Enzymes Current Drug Discovery Technologies, Vol. 4, Issue 1 https://doi.org/10.2174/157016307781115458	journal	June 2007
Activity based subcellular resolution imaging of lipases Viertler, Martin; Schittmayer, Matthias; Birner-Gruenberger, Ruth Bioorganic & Medicinal Chemistry, Vol. 20, Issue 2 https://doi.org/10.1016/j.bmc.2011.04.018	journal	January 2012
Latest trends in feedstocks for biodiesel production Pinzi, Sara; Leiva, David; López-García, Isabel Biofuels, Bioproducts and Biorefining, Vol. 8, Issue 1 https://doi.org/10.1002/bbb.1435	journal	August 2013
Manipulating Fatty Acid Biosynthesis in Microalgae for Biofuel through Protein-Protein Interactions Blatti, Jillian L.; Beld, Joris; Behnke, Craig A. PLoS ONE, Vol. 7, Issue 9 https://doi.org/10.1371/journal.pone.0042949	journal	September 2012
Proteome-Wide Reactivity Profiling Identifies Diverse Carbamate Chemotypes Tuned for Serine Hydrolase Inhibition Chang, Jae Won; Cognetta, Armand B.; Niphakis, Micah J. ACS Chemical Biology, Vol. 8, Issue 7 https://doi.org/10.1021/cb400261h	journal	May 2013
Disulphide proteomes and interactions with thioredoxin on the track towards understanding redox regulation in chloroplasts and cyanobacteria Lindahl, Marika; Kieselbach, Thomas Journal of Proteomics, Vol. 72, Issue 3 https://doi.org/10.1016/j.jprot.2009.01.003	journal	April 2009
Thioredoxin-linked processes in cyanobacteria are as numerous as in chloroplasts, but targets are different Lindahl, M.; Florencio, F. J. Proceedings of the National Academy of Sciences, Vol. 100, Issue 26, p. 16107-16112 https://doi.org/10.1073/pnas.2534397100	journal	December 2003
Protein Thiol Modifications Visualized In Vivo Leichert, Lars I.; Jakob, Ursula PLoS Biology, Vol. 2, Issue 11 https://doi.org/10.1371/journal.pbio.0020333	journal	October 2004
Live Cell Chemical Profiling of Temporal Redox Dynamics in a Photoautotrophic Cyanobacterium Sadler, Natalie C.; Melnicki, Matthew R.; Serres, Margrethe H. ACS Chemical Biology, Vol. 9, Issue 1 https://doi.org/10.1021/cb400769v	journal	October 2013
Thiol-based redox proteins in abscisic acid and methyl jasmonate signaling in Brassica napus guard cells Zhu, Mengmeng; Zhu, Ning; Song, Wen-yuan The Plant Journal, Vol. 78, Issue 3 https://doi.org/10.1111/tpj.12490	journal	April 2014
Characterization of protein redox dynamics induced during light-to-dark transitions and nutrient limitation in cyanobacteria Ansong, Charles; Sadler, Natalie C.; Hill, Eric A. Frontiers in Microbiology, Vol. 5 https://doi.org/10.3389/fmicb.2014.00325	journal	July 2014
Fifty years in the thioredoxin field and a bountiful harvest Buchanan, Bob B.; Holmgren, Arne; Jacquot, Jean-Pierre Biochimica et Biophysica Acta (BBA) - General Subjects, Vol. 1820, Issue 11 https://doi.org/10.1016/j.bbagen.2012.07.006	journal	November 2012
Sustained H2 Production Driven by Photosynthetic Water Splitting in a Unicellular Cyanobacterium Melnicki, Matthew R.; Pinchuk, Grigoriy E.; Hill, Eric A. mBio, Vol. 3, Issue 4 https://doi.org/10.1128/mBio.00197-12	journal	August 2012
Protein Phosphorylation on Ser, Thr and Tyr Residues in Cyanobacteria Zhang, Cheng-Cai; Jang, Jichan; Sakr, Samer Journal of Molecular Microbiology and Biotechnology, Vol. 9, Issue 3-4 https://doi.org/10.1159/000089644	journal	January 2005
Linking bacterial identities and ecosystem processes: can ‘omic’ analyses be more than the sum of their parts?: Toward linking bacterial identities and ecosystem processes Morales, Sergio E.; Holben, William E. FEMS Microbiology Ecology, Vol. 75, Issue 1 https://doi.org/10.1111/j.1574-6941.2010.00938.x	journal	December 2010
Efficient photoaffinity labeling of human .beta.-hexosaminidase A. Synthesis and application of 3-azi-1-[(2-acetamido-2-deoxy-1-.beta.-D-glucopyranosyl)thio]- and -galactopyranosyl)thio]butane Kuhn, Cai Steffen; Lehmann, Jochen; Sandhoff, Konrad Bioconjugate Chemistry, Vol. 3, Issue 3 https://doi.org/10.1021/bc00015a005	journal	May 1992
Nanomolar affinity, iminosugar-based chemical probes for specific labeling of lysosomal glucocerebrosidase Scherpenzeel, Monique van; van den Berg, Richard J. B. H. N.; Donker-Koopman, Wilma E. Bioorganic & Medicinal Chemistry, Vol. 18, Issue 1 https://doi.org/10.1016/j.bmc.2009.10.060	journal	January 2010
Ultrasensitive in situ visualization of active glucocerebrosidase molecules Witte, Martin D.; Kallemeijn, Wouter W.; Aten, Jan Nature Chemical Biology, Vol. 6, Issue 12 https://doi.org/10.1038/nchembio.466	journal	October 2010
Multiplexed Activity-based Protein Profiling of the Human Pathogen Aspergillus fumigatus Reveals Large Functional Changes upon Exposure to Human Serum Wiedner, Susan D.; Burnum, Kristin E.; Pederson, LeeAnna M. Journal of Biological Chemistry, Vol. 287, Issue 40 https://doi.org/10.1074/jbc.M112.394106	journal	August 2012
Profiling Serine Hydrolase Activities in Complex Proteomes ^† Kidd, Dana; Liu, Yongsheng; Cravatt, Benjamin F. Biochemistry, Vol. 40, Issue 13 https://doi.org/10.1021/bi002579j	journal	April 2001
Orlistat Is a Novel Inhibitor of Fatty Acid Synthase with Antitumor Activity Kridel, Steven J.; Axelrod, Fumiko; Rozenkrantz, Natasha Cancer Research, Vol. 64, Issue 6 https://doi.org/10.1158/0008-5472.CAN-03-3645	journal	March 2004
Inhibitor and Protein Microarrays for Activity-Based Recognition of Lipolytic Enzymes Schmidinger, Hannes; Susani-Etzerodt, Heidrun; Birner-Gruenberger, Ruth ChemBioChem, Vol. 7, Issue 3 https://doi.org/10.1002/cbic.200500276	journal	February 2006
Modulation of Fatty Acid Synthase Enzyme Activity and Expression during Hepatitis C Virus Replication Nasheri, Neda; Joyce, Michael; Rouleau, Yanouchka Chemistry & Biology, Vol. 20, Issue 4 https://doi.org/10.1016/j.chembiol.2013.03.014	journal	April 2013
A Strategy for Functional Proteomic Analysis of Glycosidase Activity from Cell Lysates Vocadlo, David J.; Bertozzi, Carolyn R. Angewandte Chemie, Vol. 116, Issue 40 https://doi.org/10.1002/ange.200454235	journal	October 2004
Organelle-Specific Activity-Based Protein Profiling in Living Cells Wiedner, Susan D.; Anderson, Lindsey N.; Sadler, Natalie C. Angewandte Chemie, Vol. 126, Issue 11 https://doi.org/10.1002/ange.201309135	journal	February 2014
An Inhibitor of Glutathione S-Transferase Omega 1 that Selectively Targets Apoptotic Cells Pace, Nicholas J.; Pimental, Daniel R.; Weerapana, Eranthie Angewandte Chemie, Vol. 124, Issue 33 https://doi.org/10.1002/ange.201203730	journal	July 2012
Design and synthesis of triglyceride analogue biotinylated suicide inhibitors for directed molecular evolution of lipolytic enzymes Deussen, H. -J; Danielsen, S.; Breinholt, J. Bioorganic & Medicinal Chemistry Letters, Vol. 10, Issue 17 https://doi.org/10.1016/s0960-894x(00)00396-6	journal	September 2000

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09 BIOMASS FUELS
activity-based protein profiling (ABPP)
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protein redox
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Title: Advancing understanding of microbial bioenergy conversion processes by activity-based protein profiling

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