Methods for determining enzymatic activity comprising heating and agitation of closed volumes
Methods for determining thermophilic enzymatic activity include heating a substrate solution in a plurality of closed volumes to a predetermined reaction temperature. Without opening the closed volumes, at least one enzyme is added, substantially simultaneously, to the closed volumes. At the predetermined reaction temperature, the closed volumes are agitated and then the activity of the at least one enzyme is determined. The methods are conducive for characterizing enzymes of high-temperature reactions, with insoluble substrates, with substrates and enzymes that do not readily intermix, and with low volumes of substrate and enzyme. Systems for characterizing the enzymes are also disclosed.
- Research Organization:
- Idaho National Laboratory (INL), Idaho Falls, ID (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC07-05ID14517
- Assignee:
- Battelle Energy Alliance, LLC (Idaho Falls, ID)
- Patent Number(s):
- 9,284,596
- Application Number:
- 14/180,161
- OSTI ID:
- 1243062
- Country of Publication:
- United States
- Language:
- English
Comparison of Two Methods for Assaying Reducing Sugars in the Determination of Carbohydrase Activities
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journal | January 2011 |
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