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Title: Protein scaffolds for selective enrichment of metal ions

Patent ·
OSTI ID:1241347

Polypeptides comprising high affinity for the uranyl ion are provided. Methods for binding uranyl using such proteins are likewise provided and can be used, for example, in methods for uranium purification or removal.

Research Organization:
The University of Chicago, Chicago, IL (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG02-07ER15865
Assignee:
The University of Chicago (Chicago, IL)
Patent Number(s):
9,255,019
Application Number:
14/394,024
OSTI ID:
1241347
Resource Relation:
Patent File Date: 2013 Mar 13
Country of Publication:
United States
Language:
English

References (21)

Affinity gradients drive copper to cellular destinations journal May 2010
Characterization of a helical protein designed from first principles journal August 1988
Crystal structure of a synthetic triple-stranded alpha-helical bundle journal February 1993
Assessing the Integrity of Designed Homomeric Parallel Three-Stranded Coiled Coils in the Presence of Metal Ions journal December 2006
Kinetics of Folding of Leucine Zipper Domains journal March 1995
Artificial Metalloenzymes: Combining the Best Features of Homogeneous and Enzymatic Catalysis journal October 2009
De Novo Design and Structural Characterization of Proteins and Metalloproteins journal June 1999
Engineering enzymes journal January 2011
Computation-Guided Backbone Grafting of a Discontinuous Motif onto a Protein Scaffold journal October 2011
Identifying important structural characteristics of arsenic resistance proteins by using designed three-stranded coiled coils journal July 2007
Site-Selective Metal Binding by Designed α-Helical Peptides journal December 2005
Expanding the utility of proteins as platforms for coordination chemistry journal April 2011
Design of functional metalloproteins journal August 2009
Stereognostic coordination chemistry. 1. The design and synthesis of chelators for the uranyl ion
  • Franczyk, Thaddeus S.; Czerwinski, Kenneth R.; Raymond, Kenneth N.
  • Journal of the American Chemical Society, Vol. 114, Issue 21, p. 8138-8146 https://doi.org/10.1021/ja00047a023
journal October 1992
Rational Design of Sequestering Agents for Plutonium and Other Actinides journal November 2003
Engineering A Uranyl-Specific Binding Protein from NikR journal February 2009
Highly Sensitive and Selective Colorimetric Sensors for Uranyl (UO 2 2+ ): Development and Comparison of Labeled and Label-Free DNAzyme-Gold Nanoparticle Systems journal October 2008
Selective binding of uranyl cation by a novel calmodulin peptide journal January 2006
Interactions of uranium and thorium with arsenazo III in an aqueous medium journal April 1997
Display of β-lactamase on the Escherichia coli surface: outer membrane phenotypes conferred by Lpp′–OmpA′–β-lactamase fusions journal January 1996
Chemical Forms of Uranium in Artificial Seawater journal February 1982