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Title: Structural Basis for a Six Nucleotide Genetic Alphabet

Authors:
; ; ; ; ;  [1];  [2]
  1. Indiana-Med
  2. (
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
NIHOTHER
OSTI Identifier:
1241053
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of the American Chemical Society; Journal Volume: 137; Journal Issue: (21) ; 06, 2015
Country of Publication:
United States
Language:
ENGLISH

Citation Formats

Georgiadis, Millie M., Singh, Isha, Kellett, Whitney F., Hoshika, Shuichi, Benner, Steven A., Richards, Nigel G.J., and IUPUI). Structural Basis for a Six Nucleotide Genetic Alphabet. United States: N. p., 2016. Web. doi:10.1021/jacs.5b03482.
Georgiadis, Millie M., Singh, Isha, Kellett, Whitney F., Hoshika, Shuichi, Benner, Steven A., Richards, Nigel G.J., & IUPUI). Structural Basis for a Six Nucleotide Genetic Alphabet. United States. doi:10.1021/jacs.5b03482.
Georgiadis, Millie M., Singh, Isha, Kellett, Whitney F., Hoshika, Shuichi, Benner, Steven A., Richards, Nigel G.J., and IUPUI). 2016. "Structural Basis for a Six Nucleotide Genetic Alphabet". United States. doi:10.1021/jacs.5b03482.
@article{osti_1241053,
title = {Structural Basis for a Six Nucleotide Genetic Alphabet},
author = {Georgiadis, Millie M. and Singh, Isha and Kellett, Whitney F. and Hoshika, Shuichi and Benner, Steven A. and Richards, Nigel G.J. and IUPUI)},
abstractNote = {},
doi = {10.1021/jacs.5b03482},
journal = {Journal of the American Chemical Society},
number = (21) ; 06, 2015,
volume = 137,
place = {United States},
year = 2016,
month = 7
}
  • Patients who have pseudohypoparathyroidism type I associated with Albright hereditary osteodystrophy commonly have a genetic deficiency of the ..cap alpha.. subunit of the G protein that stimulated adenylyl cyclase ..cap alpha..G/sub s/. To discover the molecular mechanism that causes ..cap alpha..G/sub s/ deficiency in these patients, the authors examined eight kindreds with one or more members affected with Albright hereditary osteodystrophy or pseudohypoparathyroidism and ..cap alpha..G/sub s/ deficiency. In these families, ..cap alpha..G/sub s/, deficiency and the Albright hereditary osteodystrophy phenotype were transmitted together in a dominant inheritance pattern. Using a cDNA hybridization probe for ..cap alpha..G/sub s/, restriction analysismore » with several analysis with several endonucleases showed no abnormalities of restriction fragments or gene dosage. RNA blot and dot blot analysis of total RNA from cultured fibroblasts obtained from the patients revealed approx. 50% reduced mRNA levels for ..cap alpha..G/sub s/ in affected members of six of the pedigrees but normal levels in affected members of the two other pedigrees, compared to mRNA levels in fibroblasts from unaffected individuals. By contrast, mRNA levels encoding the ..cap alpha.. subunit of the G protein that inhibits adenylyl cyclase were not altered. These findings suggest that several molecular mechanisms produce ..cap alpha..G/sub s/ deficiency in patients with pseudohypoparathyroidism type Ia and that major gene rearrangements or deletions are not a common cause for ..cap alpha..G/sub s/ deficiency in pseudohypoparathyroidism type I.« less
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