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Title: Methods of producing protoporphyrin IX and bacterial mutants therefor

Abstract

The presently disclosed inventive concepts are directed in certain embodiments to a method of producing protoporphyrin IX by (1) cultivating a strain of Shewanella bacteria in a culture medium under conditions suitable for growth thereof, and (2) recovering the protoporphyrin IX from the culture medium. The strain of Shewanella bacteria comprises at least one mutant hemH gene which is incapable of normal expression, thereby causing an accumulation of protoporphyrin IX. In certain embodiments of the method, the strain of Shewanella bacteria is a strain of S. loihica, and more specifically may be S. loihica PV-4. In certain embodiments, the mutant hemH gene of the strain of Shewanella bacteria may be a mutant of shew_2229 and/or of shew_1140. In other embodiments, the presently disclosed inventive concepts are directed to mutant strains of Shewanella bacteria having at least one mutant hemH gene which is incapable of normal expression, thereby causing an accumulation of protoporphyrin IX during cultivation of the bacteria. In certain embodiments the strain of Shewanella bacteria is a strain of S. loihica, and more specifically may be S. loihica PV-4. In certain embodiments, the mutant hemH gene of the strain of Shewanella bacteria may be a mutant of shew_2229 and/ormore » shew_1140.« less

Inventors:
; ; ;
Publication Date:
Research Org.:
The Board of Regents of the University of Oklahoma, Norman, OK (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1241022
Patent Number(s):
9,273,334
Application Number:
14/349,171
Assignee:
The Board of Regents of the University of Oklahoma (Norman, OK) CHO
DOE Contract Number:  
FG02-07ER64383
Resource Type:
Patent
Resource Relation:
Patent File Date: 2014 Mar 14
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES

Citation Formats

Zhou, Jizhong, Qiu, Dongru, He, Zhili, and Xie, Ming. Methods of producing protoporphyrin IX and bacterial mutants therefor. United States: N. p., 2016. Web.
Zhou, Jizhong, Qiu, Dongru, He, Zhili, & Xie, Ming. Methods of producing protoporphyrin IX and bacterial mutants therefor. United States.
Zhou, Jizhong, Qiu, Dongru, He, Zhili, and Xie, Ming. Tue . "Methods of producing protoporphyrin IX and bacterial mutants therefor". United States. doi:. https://www.osti.gov/servlets/purl/1241022.
@article{osti_1241022,
title = {Methods of producing protoporphyrin IX and bacterial mutants therefor},
author = {Zhou, Jizhong and Qiu, Dongru and He, Zhili and Xie, Ming},
abstractNote = {The presently disclosed inventive concepts are directed in certain embodiments to a method of producing protoporphyrin IX by (1) cultivating a strain of Shewanella bacteria in a culture medium under conditions suitable for growth thereof, and (2) recovering the protoporphyrin IX from the culture medium. The strain of Shewanella bacteria comprises at least one mutant hemH gene which is incapable of normal expression, thereby causing an accumulation of protoporphyrin IX. In certain embodiments of the method, the strain of Shewanella bacteria is a strain of S. loihica, and more specifically may be S. loihica PV-4. In certain embodiments, the mutant hemH gene of the strain of Shewanella bacteria may be a mutant of shew_2229 and/or of shew_1140. In other embodiments, the presently disclosed inventive concepts are directed to mutant strains of Shewanella bacteria having at least one mutant hemH gene which is incapable of normal expression, thereby causing an accumulation of protoporphyrin IX during cultivation of the bacteria. In certain embodiments the strain of Shewanella bacteria is a strain of S. loihica, and more specifically may be S. loihica PV-4. In certain embodiments, the mutant hemH gene of the strain of Shewanella bacteria may be a mutant of shew_2229 and/or shew_1140.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 01 00:00:00 EST 2016},
month = {Tue Mar 01 00:00:00 EST 2016}
}

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Works referenced in this record:

Electron transfer mechanism in Shewanella loihica PV-4 biofilms formed at graphite electrode
journal, October 2012


Accumulation of protoporphyrin IX in light-sensitive mutants of Escherichia coli
journal, October 1992