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Title: Preparation of highly luminescent and color tunable carbon nanodots under visible light excitation for in vitro and in vivo bio-imaging

Abstract

Carbon nanodots (CDs) have generated enormous excitement because of their superiority in water solubility, chemical inertness, low toxicity, ease of functionalization and resistance to photobleaching. Here we report a facile thermal pyrolysis route to prepare CDs with high quantum yield (QY) using citric acid as the carbon source and ethylene diamine derivatives (EDAs) including triethylenetetramine (TETA), tetraethylenepentamine (TEPA) and polyene polyamine (PEPA) as the passivation agents. We find that the CDs prepared from EDAs, such as TETA, TEPA and PEPA, show relatively high photoluminescence (PL) QY (11.4, 10.6, and 9.8%, respectively) at 1 ex of 465 nm. The cytotoxicity of the CDs has been investigated through in vitro and in vivo bio-imaging studies. The results indicate that these CDs possess low toxicity and good biocompatibility. As a result, the unique properties such as the high PL QY at large excitation wave length and the low toxicity of the resulting CDs make them promising fluorescent nanoprobes for applications in optical bio-imaging and biosensing.

Authors:
 [1];  [2];  [2];  [3];  [3];  [2];  [4];  [5];  [2]
  1. Beijing Univ. of Technology, Beijing (China)
  2. Chinese Academy of Sciences, Jilin, (People's Republic of China)
  3. Chinese Academy of Sciences, Jilin (People's Republic of China)
  4. Beijing Univ. of Technology, Beijing (People's Republic of China); Chinese Academy of Sciences, Jilin, (People's Republic of China)
  5. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Univ. of New Mexico, Albuquerque, NM (United States)
Publication Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1240098
Report Number(s):
SAND2016-0800J
Journal ID: ISSN 0884-2914; 618955
Grant/Contract Number:  
AC04-94AL85000
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Journal of Materials Research
Additional Journal Information:
Journal Volume: 30; Journal Issue: 22; Journal ID: ISSN 0884-2914
Publisher:
Materials Research Society
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE

Citation Formats

Zheng, Min, Li, Jing, Xie, Zhigang, Qu, Dan, Miao, Xiang, Jing, Xiabing, Sun, Zaicheng, Fan, Hongyou, and Liu, Shi. Preparation of highly luminescent and color tunable carbon nanodots under visible light excitation for in vitro and in vivo bio-imaging. United States: N. p., 2015. Web. doi:10.1557/jmr.2015.323.
Zheng, Min, Li, Jing, Xie, Zhigang, Qu, Dan, Miao, Xiang, Jing, Xiabing, Sun, Zaicheng, Fan, Hongyou, & Liu, Shi. Preparation of highly luminescent and color tunable carbon nanodots under visible light excitation for in vitro and in vivo bio-imaging. United States. https://doi.org/10.1557/jmr.2015.323
Zheng, Min, Li, Jing, Xie, Zhigang, Qu, Dan, Miao, Xiang, Jing, Xiabing, Sun, Zaicheng, Fan, Hongyou, and Liu, Shi. Tue . "Preparation of highly luminescent and color tunable carbon nanodots under visible light excitation for in vitro and in vivo bio-imaging". United States. https://doi.org/10.1557/jmr.2015.323. https://www.osti.gov/servlets/purl/1240098.
@article{osti_1240098,
title = {Preparation of highly luminescent and color tunable carbon nanodots under visible light excitation for in vitro and in vivo bio-imaging},
author = {Zheng, Min and Li, Jing and Xie, Zhigang and Qu, Dan and Miao, Xiang and Jing, Xiabing and Sun, Zaicheng and Fan, Hongyou and Liu, Shi},
abstractNote = {Carbon nanodots (CDs) have generated enormous excitement because of their superiority in water solubility, chemical inertness, low toxicity, ease of functionalization and resistance to photobleaching. Here we report a facile thermal pyrolysis route to prepare CDs with high quantum yield (QY) using citric acid as the carbon source and ethylene diamine derivatives (EDAs) including triethylenetetramine (TETA), tetraethylenepentamine (TEPA) and polyene polyamine (PEPA) as the passivation agents. We find that the CDs prepared from EDAs, such as TETA, TEPA and PEPA, show relatively high photoluminescence (PL) QY (11.4, 10.6, and 9.8%, respectively) at 1ex of 465 nm. The cytotoxicity of the CDs has been investigated through in vitro and in vivo bio-imaging studies. The results indicate that these CDs possess low toxicity and good biocompatibility. As a result, the unique properties such as the high PL QY at large excitation wave length and the low toxicity of the resulting CDs make them promising fluorescent nanoprobes for applications in optical bio-imaging and biosensing.},
doi = {10.1557/jmr.2015.323},
url = {https://www.osti.gov/biblio/1240098}, journal = {Journal of Materials Research},
issn = {0884-2914},
number = 22,
volume = 30,
place = {United States},
year = {2015},
month = {11}
}

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Works referenced in this record:

Water-Soluble Fluorescent Carbon Quantum Dots and Photocatalyst Design
journal, June 2010


Color-Switchable Electroluminescence of Carbon Dot Light-Emitting Diodes
journal, November 2013


Highly luminescent carbon nanodots by microwave-assisted pyrolysis
journal, January 2012


Nanosized Carbon Particles From Natural Gas Soot
journal, July 2009


Carbon Nanoparticles as Visible-Light Photocatalysts for Efficient CO 2 Conversion and Beyond
journal, April 2011


Carbon Dots for Multiphoton Bioimaging
journal, September 2007


Microwave synthesis of fluorescent carbon nanoparticles with electrochemiluminescence properties
journal, January 2009


Electrophoretic Analysis and Purification of Fluorescent Single-Walled Carbon Nanotube Fragments
journal, October 2004


Facile access to versatile fluorescent carbon dots toward light-emitting diodes
journal, January 2012


Metal-free efficient photocatalyst for stable visible water splitting via a two-electron pathway
journal, February 2015


Highly Photoluminescent Carbon Dots for Multicolor Patterning, Sensors, and Bioimaging
journal, February 2013


Luminescent Carbon Nanodots: Emergent Nanolights
journal, August 2010


Tailoring color emissions from N-doped graphene quantum dots for bioimaging applications
journal, December 2015


Formation Mechanism of Carbogenic Nanoparticles with Dual Photoluminescence Emission
journal, December 2011


Large scale electrochemical synthesis of high quality carbon nanodots and their photocatalytic property
journal, January 2012


Three Colors Emission from S,N Co-doped Graphene Quantum Dots for Visible Light H 2 Production and Bioimaging
journal, January 2015


Quantum-Sized Carbon Dots for Bright and Colorful Photoluminescence
journal, June 2006


Carbon “quantum” dots for optical bioimaging
journal, January 2013


Carbon Dots for Optical Imaging in Vivo
journal, August 2009


Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions
journal, March 2012