RNA-guided transcriptional regulation

Church, George M.; Mali, Prashant G.; Esvelt, Kevin M.

RNA-guided transcriptional regulation

Patent · 23 February 2016

OSTI ID:1239817

Church, George M.; Mali, Prashant G.; Esvelt, Kevin M.

Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein or domain are expressed, wherein the one or more RNAs, the nuclease-null Cas9 protein and the transcriptional regulator protein or domain co-localize to the DNA and wherein the transcriptional regulator protein or domain regulates expression of the target nucleic acid.

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Research Organization:: Harvard College, Cambridge, MA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-02ER63445

Assignee:: President and Fellows of Harvard College (Cambridge, MA)

Patent Number(s):: 9,267,135

Application Number:: 14/319,289

OSTI ID:: 1239817

Country of Publication:: United States

Language:: English

References (18)

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