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Title: Quantitative mapping of zinc fluxes in the mammalian egg reveals the origin of fertilization-induced zinc sparks

Abstract

Fertilization of a mammalian egg initiates a series of 'zinc sparks' that are necessary to induce the egg-to-embryo transition. Despite the importance of these zinc-efflux events little is known about their origin. To understand the molecular mechanism of the zinc spark we combined four physical approaches that resolve zinc distributions in single cells: a chemical probe for dynamic live-cell fluorescence imaging and a combination of scanning transmission electron microscopy with energy-dispersive spectroscopy, X-ray fluorescence microscopy and three-dimensional elemental tomography for high-resolution elemental mapping. We show that the zinc spark arises from a system of thousands of zinc-loaded vesicles, each of which contains, on average, 10(6) zinc atoms. These vesicles undergo dynamic movement during oocyte maturation and exocytosis at the time of fertilization. The discovery of these vesicles and the demonstration that zinc sparks originate from them provides a quantitative framework for understanding how zinc fluxes regulate cellular processes

Authors:
; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1239606
DOE Contract Number:  
AC02-06CH11357
Resource Type:
Journal Article
Journal Name:
Nature Chemistry
Additional Journal Information:
Journal Volume: 7; Journal Issue: 2; Journal ID: ISSN 1755-4330
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; COPPER; CELLS; CHEMISTRY; OOCYTES; POTENTIALS; SPERM; TRAFFICKING; TRANSITION; TRANSPORTER

Citation Formats

Que, Emily L., Bleher, Reiner, Duncan, Francesca E., Kong, Betty Y., Gleber, Sophie C., Vogt, Stefan, Chen, Si, Garwin, Seth A., Bayer, Amanda R., Dravid, Vinayak P., Woodruff, Teresa K., and O'Halloran, Thomas V. Quantitative mapping of zinc fluxes in the mammalian egg reveals the origin of fertilization-induced zinc sparks. United States: N. p., 2014. Web. doi:10.1038/NCHEM.2133.
Que, Emily L., Bleher, Reiner, Duncan, Francesca E., Kong, Betty Y., Gleber, Sophie C., Vogt, Stefan, Chen, Si, Garwin, Seth A., Bayer, Amanda R., Dravid, Vinayak P., Woodruff, Teresa K., & O'Halloran, Thomas V. Quantitative mapping of zinc fluxes in the mammalian egg reveals the origin of fertilization-induced zinc sparks. United States. doi:10.1038/NCHEM.2133.
Que, Emily L., Bleher, Reiner, Duncan, Francesca E., Kong, Betty Y., Gleber, Sophie C., Vogt, Stefan, Chen, Si, Garwin, Seth A., Bayer, Amanda R., Dravid, Vinayak P., Woodruff, Teresa K., and O'Halloran, Thomas V. Mon . "Quantitative mapping of zinc fluxes in the mammalian egg reveals the origin of fertilization-induced zinc sparks". United States. doi:10.1038/NCHEM.2133.
@article{osti_1239606,
title = {Quantitative mapping of zinc fluxes in the mammalian egg reveals the origin of fertilization-induced zinc sparks},
author = {Que, Emily L. and Bleher, Reiner and Duncan, Francesca E. and Kong, Betty Y. and Gleber, Sophie C. and Vogt, Stefan and Chen, Si and Garwin, Seth A. and Bayer, Amanda R. and Dravid, Vinayak P. and Woodruff, Teresa K. and O'Halloran, Thomas V.},
abstractNote = {Fertilization of a mammalian egg initiates a series of 'zinc sparks' that are necessary to induce the egg-to-embryo transition. Despite the importance of these zinc-efflux events little is known about their origin. To understand the molecular mechanism of the zinc spark we combined four physical approaches that resolve zinc distributions in single cells: a chemical probe for dynamic live-cell fluorescence imaging and a combination of scanning transmission electron microscopy with energy-dispersive spectroscopy, X-ray fluorescence microscopy and three-dimensional elemental tomography for high-resolution elemental mapping. We show that the zinc spark arises from a system of thousands of zinc-loaded vesicles, each of which contains, on average, 10(6) zinc atoms. These vesicles undergo dynamic movement during oocyte maturation and exocytosis at the time of fertilization. The discovery of these vesicles and the demonstration that zinc sparks originate from them provides a quantitative framework for understanding how zinc fluxes regulate cellular processes},
doi = {10.1038/NCHEM.2133},
journal = {Nature Chemistry},
issn = {1755-4330},
number = 2,
volume = 7,
place = {United States},
year = {2014},
month = {12}
}