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Title: Structural and Functional Characterization of the Hazelnut Allergen Cor a 8

Authors:
; ; ; ; ; ; ;  [1];  [2];  [2]
  1. Polish
  2. (
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
DOE - BASIC ENERGY SCIENCES
OSTI Identifier:
1239421
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Agricultural and Food Chemistry; Journal Volume: 63; Journal Issue: 41
Country of Publication:
United States
Language:
ENGLISH

Citation Formats

Offermann, Lesa R., Bublin, Merima, Perdue, Makenzie L., Pfeifer, Sabine, Dubiela, Pawel, Borowski, Tomasz, Chruszcz, Maksymilian, Hoffmann-Sommergruber, Karin, Med U. Vienna), and SC). Structural and Functional Characterization of the Hazelnut Allergen Cor a 8. United States: N. p., 2016. Web. doi:10.1021/acs.jafc.5b03534.
Offermann, Lesa R., Bublin, Merima, Perdue, Makenzie L., Pfeifer, Sabine, Dubiela, Pawel, Borowski, Tomasz, Chruszcz, Maksymilian, Hoffmann-Sommergruber, Karin, Med U. Vienna), & SC). Structural and Functional Characterization of the Hazelnut Allergen Cor a 8. United States. doi:10.1021/acs.jafc.5b03534.
Offermann, Lesa R., Bublin, Merima, Perdue, Makenzie L., Pfeifer, Sabine, Dubiela, Pawel, Borowski, Tomasz, Chruszcz, Maksymilian, Hoffmann-Sommergruber, Karin, Med U. Vienna), and SC). 2016. "Structural and Functional Characterization of the Hazelnut Allergen Cor a 8". United States. doi:10.1021/acs.jafc.5b03534.
@article{osti_1239421,
title = {Structural and Functional Characterization of the Hazelnut Allergen Cor a 8},
author = {Offermann, Lesa R. and Bublin, Merima and Perdue, Makenzie L. and Pfeifer, Sabine and Dubiela, Pawel and Borowski, Tomasz and Chruszcz, Maksymilian and Hoffmann-Sommergruber, Karin and Med U. Vienna) and SC)},
abstractNote = {},
doi = {10.1021/acs.jafc.5b03534},
journal = {Journal of Agricultural and Food Chemistry},
number = 41,
volume = 63,
place = {United States},
year = 2016,
month = 6
}
  • Nonspecific lipid transfer proteins (nsLTPs) are basic proteins, stabilized by four disulfide bonds, and are expressed throughout the plant kingdom. These proteins are also known as important allergens in fruits and tree nuts. In this study, the nsLTP from hazelnuts, Cor a 8, was purified and its crystal structure determined. The protein is stable at low pH and refolds after thermal denaturation. Molecular dynamics simulations were used to provide an insight into conformational changes of Cor a 8 upon ligand binding. When known epitope areas from Pru p 3 were compared to those of Cor a 8, differences were obvious,more » which may contribute to limited cross-reactivity between peach and hazelnut allergens. The differences in epitope regions may contribute to limited cross-reactivity between Cor a 8 and nsLTPs from other plant sources. The structure of Cor a 8 represents the first resolved structure of a hazelnut allergen.« less
  • Both allergen and ozone exposure increase asthma symptoms and airway responsiveness in children. Little is known about how these inhalants may differentially modify airway responsiveness in large proximal as compared to small distal airways. We evaluated whether bronchi and respiratory bronchioles from infant monkeys exposed episodically to allergen and/or ozone differentially develop intrinsic hyperresponsiveness to methacholine and whether eosinophils and/or pulmonary neuroendocrine cells play a role. Infant monkeys were exposed episodically for 5 months to: (1) filtered air, (2) aerosolized house dust mite allergen, (3) ozone 0.5 ppm, or (4) house dust mite allergen + ozone. Studying the function/structure relationshipmore » of the same lung slices, we evaluated methacholine airway responsiveness and histology of bronchi and respiratory bronchioles. In bronchi, intrinsic responsiveness was increased by allergen exposure, an effect reduced by bombesin antagonist. In respiratory bronchioles, intrinsic airway responsiveness was increased by allergen + ozone exposure. Eosinophils were increased by allergen and allergen + ozone exposure in bronchi and by allergen exposure in respiratory bronchioles. In both airways, exposure to allergen + ozone resulted in fewer tissue eosinophils than did allergen exposure alone. In bronchi, but not in respiratory bronchioles, the number of eosinophils and neuroendocrine cells correlated with airway responsiveness. We conclude that episodically exposing infant monkeys to house dust mite allergen with or without ozone increased intrinsic airway responsiveness to methacholine in bronchi differently than in respiratory bronchioles. In bronchi, eosinophils and neuroendocrine cells may play a role in the development of airway hyperresponsiveness.« less