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Title: Metaproteomics Reveals Functional Shifts in Microbial and Human Proteins During Infant Gut Colonization Case

Abstract

The microbial colonization of the human gastrointestinal tract plays an important role in establishing health and homeostasis. However, the time-dependent functional signatures of microbial and human proteins during early colonization of the gut have yet to be determined. Thus, we employed shotgun proteomics to simultaneously monitor microbial and human proteins in fecal samples from a preterm infant during the first month of life. Microbial community complexity and functions increased over time, with compositional changes that were consistent with previous metagenomic and rRNA gene data indicating three distinct colonization phases. Overall microbial community functions were established relatively early in development and remained stable. Human proteins detected included those responsible for epithelial barrier function and antimicrobial activity. Some neutrophil-derived proteins increased in abundance early in the study period, suggesting activation of the innate immune system. Moreover, abundances of cytoskeletal and mucin proteins increased later in the time course, suggestive of subsequent adjustment to the increased microbial load. Our study provides the first snapshot of coordinated human and microbial protein expression in the infant gut during early development.

Authors:
 [1];  [2];  [1];  [3];  [3];  [4];
  1. Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. Univ. of California, Berkeley, CA (United States)
  4. Univ. of Pittsburgh, PA (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1237608
Grant/Contract Number:
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Proteomics (Online)
Additional Journal Information:
Journal Name: Proteomics (Online); Journal Volume: 15; Journal Issue: 20; Journal ID: ISSN 1615-9861
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Infant gut; Metaproteomics; Microbial colonization; Microbiome; Systems biology

Citation Formats

Young, Jacque C., Pan, Chongle, Adams, Rachel M., Brooks, Brandon, Banfield, Jillian F., Morowitz, Michael J., and Robert L. Hettich. Metaproteomics Reveals Functional Shifts in Microbial and Human Proteins During Infant Gut Colonization Case. United States: N. p., 2015. Web. doi:10.1002/pmic.201400563.
Young, Jacque C., Pan, Chongle, Adams, Rachel M., Brooks, Brandon, Banfield, Jillian F., Morowitz, Michael J., & Robert L. Hettich. Metaproteomics Reveals Functional Shifts in Microbial and Human Proteins During Infant Gut Colonization Case. United States. doi:10.1002/pmic.201400563.
Young, Jacque C., Pan, Chongle, Adams, Rachel M., Brooks, Brandon, Banfield, Jillian F., Morowitz, Michael J., and Robert L. Hettich. Thu . "Metaproteomics Reveals Functional Shifts in Microbial and Human Proteins During Infant Gut Colonization Case". United States. doi:10.1002/pmic.201400563. https://www.osti.gov/servlets/purl/1237608.
@article{osti_1237608,
title = {Metaproteomics Reveals Functional Shifts in Microbial and Human Proteins During Infant Gut Colonization Case},
author = {Young, Jacque C. and Pan, Chongle and Adams, Rachel M. and Brooks, Brandon and Banfield, Jillian F. and Morowitz, Michael J. and Robert L. Hettich},
abstractNote = {The microbial colonization of the human gastrointestinal tract plays an important role in establishing health and homeostasis. However, the time-dependent functional signatures of microbial and human proteins during early colonization of the gut have yet to be determined. Thus, we employed shotgun proteomics to simultaneously monitor microbial and human proteins in fecal samples from a preterm infant during the first month of life. Microbial community complexity and functions increased over time, with compositional changes that were consistent with previous metagenomic and rRNA gene data indicating three distinct colonization phases. Overall microbial community functions were established relatively early in development and remained stable. Human proteins detected included those responsible for epithelial barrier function and antimicrobial activity. Some neutrophil-derived proteins increased in abundance early in the study period, suggesting activation of the innate immune system. Moreover, abundances of cytoskeletal and mucin proteins increased later in the time course, suggestive of subsequent adjustment to the increased microbial load. Our study provides the first snapshot of coordinated human and microbial protein expression in the infant gut during early development.},
doi = {10.1002/pmic.201400563},
journal = {Proteomics (Online)},
number = 20,
volume = 15,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 2015},
month = {Thu Jan 01 00:00:00 EST 2015}
}

Journal Article:
Free Publicly Available Full Text
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Cited by: 14 works
Citation information provided by
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