skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Accounting for all sugars produced during integrated production of ethanol from lignocellulosic biomass

Journal Article · · Bioresource Technology
 [1];  [1];  [2];  [1];  [1]
  1. National Renewable Energy Lab. (NREL), Golden, CO (United States). National Bioenergy Center
  2. P2 Science, Woodbridge, CT (United States)

This study explored integrated conversion of corn stover to ethanol and highlights techniques for accurate yield calculations. Acid pretreated corn stover (PCS) produced in a pilot-scale reactor was enzymatically hydrolyzed and the resulting sugars were fermented to ethanol by the glucose–xylose fermenting bacteria, Zymomonas mobilis 8b. The calculations account for high solids operation and oligomeric sugars produced during pretreatment, enzymatic hydrolysis, and fermentation, which, if not accounted for, leads to overestimating ethanol yields. The calculations are illustrated for enzymatic hydrolysis and fermentation of PCS at 17.5% and 20.0% total solids achieving 80.1% and 77.9% conversion of cellulose and xylan to ethanol and ethanol titers of 63 g/L and 69 g/L, respectively. In the future, these techniques, including the TEA results, will be applied to fully integrated pilot-scale runs.

Research Organization:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office (BETO)
Grant/Contract Number:
AC36-08GO28308
OSTI ID:
1237439
Alternate ID(s):
OSTI ID: 1358840
Report Number(s):
NREL/JA-5100-65494
Journal Information:
Bioresource Technology, Vol. 205, Issue C; ISSN 0960-8524
Publisher:
ElsevierCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 14 works
Citation information provided by
Web of Science

References (14)

Challenges in enzymatic hydrolysis and fermentation of pretreated Arundo donax revealed by a comparison between SHF and SSF journal October 2012
A review of cellulosic biofuel commercial-scale projects in the United States journal February 2013
The impacts of deacetylation prior to dilute acid pretreatment on the bioethanol process journal January 2012
A comparison between SHF and SSSF processes from cardoon for ethanol production journal July 2015
Inhibition of growth of Zymomonas mobilis by model compounds found in lignocellulosic hydrolysates journal January 2013
Model-Based Fed-Batch for High-Solids Enzymatic Cellulose Hydrolysis journal May 2008
Process Design and Economics for Biochemical Conversion of Lignocellulosic Biomass to Ethanol: Dilute-Acid Pretreatment and Enzymatic Hydrolysis of Corn Stover report March 2011
Influence of High Solid Concentration on Enzymatic Hydrolysis and Fermentation of Steam-Exploded Corn Stover Biomass journal July 2008
Different process configurations for bioethanol production from pretreated olive pruning biomass journal March 2011
Performance of a newly developed integrant of Zymomonas mobilis for ethanol production on corn stover hydrolysate journal February 2004
Characterization of pilot-scale dilute acid pretreatment performance using deacetylated corn stover journal January 2014
Effect of mechanical disruption on the effectiveness of three reactors used for dilute acid pretreatment of corn stover Part 1: chemical and physical substrate analysis journal January 2014
Calculating sugar yields in high solids hydrolysis of biomass journal February 2011
Simultaneous saccharification and co-fermentation of aqueous ammonia pretreated corn stover with an engineered Saccharomyces cerevisiae SyBE005 journal October 2014

Cited By (5)

Enzyme production and oil palm empty fruit bunch bioconversion to ethanol using a hybrid yeast strain journal September 2019
Zymomonas mobilis as a model system for production of biofuels and biochemicals journal September 2016
Ternary Blended Chitosan/Chitin/ $$\hbox {FE}_{3}\hbox {O}_{4}$$ FE 3 O 4 Nanosupport for Lipase Activation and Stabilization journal February 2019
Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis journal February 2018
Enhanced lipid production by Rhodosporidium toruloides using different fed-batch feeding strategies with lignocellulosic hydrolysate as the sole carbon source journal June 2016