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Title: Compositions and methods for pathogen transport

Abstract

This disclosure provides a method for transporting a pathogen under ambient conditions, by culturing the pathogen with an amoeba under conditions that favor the incorporation of the pathogen into a trophozoite, starving the amoeba until it encysts, then culturing under conditions that favor conversion of the amoeba back to a trophozoite. In one aspect, the conditions that favor incorporation of the pathogen into the cyst of the amoeba comprises contacting the pathogen with the amoeba in an iron rich environment. Virus and/or bacteria are pathogens that can be transported by the disclosed method. Amoeba that are useful in the disclosed methods include, without limitation Acanthamoeba castellanii, Hartmannella vermiformis and Naegleria gruberi. The disclosed methods have utility in: transporting pathogens from military field hospitals and clinics to the laboratory; transporting pathogens from global satellite laboratories to clinical laboratories; long term storage of pathogens; enriching contaminated patient samples for pathogens of interest; biosurveillance and detection efforts.

Inventors:
;
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1236565
Patent Number(s):
9,243,222
Application Number:
14/148,484
Assignee:
Lawrence Livermore National Security, LLC LLNL
DOE Contract Number:  
AC52-07NA27344
Resource Type:
Patent
Resource Relation:
Patent File Date: 2014 Jan 06
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats

El-Etr, Sahar, and Farquar, George R. Compositions and methods for pathogen transport. United States: N. p., 2016. Web.
El-Etr, Sahar, & Farquar, George R. Compositions and methods for pathogen transport. United States.
El-Etr, Sahar, and Farquar, George R. Tue . "Compositions and methods for pathogen transport". United States. https://www.osti.gov/servlets/purl/1236565.
@article{osti_1236565,
title = {Compositions and methods for pathogen transport},
author = {El-Etr, Sahar and Farquar, George R.},
abstractNote = {This disclosure provides a method for transporting a pathogen under ambient conditions, by culturing the pathogen with an amoeba under conditions that favor the incorporation of the pathogen into a trophozoite, starving the amoeba until it encysts, then culturing under conditions that favor conversion of the amoeba back to a trophozoite. In one aspect, the conditions that favor incorporation of the pathogen into the cyst of the amoeba comprises contacting the pathogen with the amoeba in an iron rich environment. Virus and/or bacteria are pathogens that can be transported by the disclosed method. Amoeba that are useful in the disclosed methods include, without limitation Acanthamoeba castellanii, Hartmannella vermiformis and Naegleria gruberi. The disclosed methods have utility in: transporting pathogens from military field hospitals and clinics to the laboratory; transporting pathogens from global satellite laboratories to clinical laboratories; long term storage of pathogens; enriching contaminated patient samples for pathogens of interest; biosurveillance and detection efforts.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {1}
}

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Works referenced in this record:

Necessity of iron for the alternative respiratory pathway in acanthamoeba castellanii
journal, March 1980

  • Hryniewiecka, L.; Jenek, J.; Michejda, J. W.
  • Biochemical and Biophysical Research Communications, Vol. 93, Issue 1, p. 141-148
  • DOI: 10.1016/S0006-291X(80)80257-9

Acanthamoeba castellanii: Growth, encystment, excystment and biocide susceptibility
journal, January 1998


The Neff strain of Acanthamoeba castellanii, a tool for testing the virulence of Mycobacterium kansasii
journal, May 2007

  • Goy, Genevi√®ve; Thomas, Vincent; Rimann, Katia
  • Research in Microbiology, Vol. 158, Issue 4, p. 393-397
  • DOI: 10.1016/j.resmic.2007.01.003