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Title: Overcoming substrate limitations for improved production of ethylene in E. coli

Abstract

Ethylene is an important industrial compound for the production of a wide variety of plastics and chemicals. At present, ethylene production involves steam cracking of a fossil-based feedstock, representing the highest CO 2-emitting process in the chemical industry. Biological ethylene production can be achieved via expression of a single protein, the ethylene-forming enzyme (EFE), found in some bacteria and fungi; it has the potential to provide a sustainable alternative to steam cracking, provided that significant increases in productivity can be achieved. A key barrier is determining factors that influence the availability of substrates for the EFE reaction in potential microbial hosts. In the presence of O 2, EFE catalyzes ethylene formation from the substrates α-ketoglutarate (AKG) and arginine. The concentrations of AKG, a key TCA cycle intermediate, and arginine are tightly controlled by an intricate regulatory system that coordinates carbon and nitrogen metabolism. Thus, reliably predicting which genetic changes will ultimately lead to increased AKG and arginine availability is challenging.

Authors:
 [1];  [1];  [2];  [3];  [2]
  1. National Renewable Energy Lab. (NREL), Golden, CO (United States); Univ. of Colorado, Boulder, CO (United States)
  2. National Renewable Energy Lab. (NREL), Golden, CO (United States)
  3. Univ. of Colorado, Boulder, CO (United States)
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1236036
Report Number(s):
NREL/JA-2700-63804
Journal ID: ISSN 1754-6834
Grant/Contract Number:  
AC36-08GO28308
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 9; Journal Issue: 1; Related Information: Biotechnology for Biofuels; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; ethylene; ethylene-forming enzyme; arginine; α-ketoglutarate; E. coli

Citation Formats

Lynch, Sean, Eckert, Carrie, Yu, Jianping, Gill, Ryan, and Maness, Pin -Ching. Overcoming substrate limitations for improved production of ethylene in E. coli. United States: N. p., 2016. Web. doi:10.1186/s13068-015-0413-x.
Lynch, Sean, Eckert, Carrie, Yu, Jianping, Gill, Ryan, & Maness, Pin -Ching. Overcoming substrate limitations for improved production of ethylene in E. coli. United States. doi:10.1186/s13068-015-0413-x.
Lynch, Sean, Eckert, Carrie, Yu, Jianping, Gill, Ryan, and Maness, Pin -Ching. Mon . "Overcoming substrate limitations for improved production of ethylene in E. coli". United States. doi:10.1186/s13068-015-0413-x. https://www.osti.gov/servlets/purl/1236036.
@article{osti_1236036,
title = {Overcoming substrate limitations for improved production of ethylene in E. coli},
author = {Lynch, Sean and Eckert, Carrie and Yu, Jianping and Gill, Ryan and Maness, Pin -Ching},
abstractNote = {Ethylene is an important industrial compound for the production of a wide variety of plastics and chemicals. At present, ethylene production involves steam cracking of a fossil-based feedstock, representing the highest CO2-emitting process in the chemical industry. Biological ethylene production can be achieved via expression of a single protein, the ethylene-forming enzyme (EFE), found in some bacteria and fungi; it has the potential to provide a sustainable alternative to steam cracking, provided that significant increases in productivity can be achieved. A key barrier is determining factors that influence the availability of substrates for the EFE reaction in potential microbial hosts. In the presence of O2, EFE catalyzes ethylene formation from the substrates α-ketoglutarate (AKG) and arginine. The concentrations of AKG, a key TCA cycle intermediate, and arginine are tightly controlled by an intricate regulatory system that coordinates carbon and nitrogen metabolism. Thus, reliably predicting which genetic changes will ultimately lead to increased AKG and arginine availability is challenging.},
doi = {10.1186/s13068-015-0413-x},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 9,
place = {United States},
year = {Mon Jan 04 00:00:00 EST 2016},
month = {Mon Jan 04 00:00:00 EST 2016}
}

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Free Publicly Available Full Text
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Cited by: 5 works
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Works referenced in this record:

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