skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Thermophilic and thermoacidophilic glycosylation genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods


Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for glycosylating and/or post-translationally modifying proteins using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.

; ; ; ;
Publication Date:
Research Org.:
Idaho National Lab. (INL), Idaho Falls, ID (United States)
Sponsoring Org.:
OSTI Identifier:
Patent Number(s):
Application Number:
DOE Contract Number:  
Resource Type:
Resource Relation:
Patent File Date: 2009 Feb 26
Country of Publication:
United States

Citation Formats

Thompson, David N., Apel, William A., Thompson, Vicki S., Reed, David W., and Lacey, Jeffrey A. Thermophilic and thermoacidophilic glycosylation genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods. United States: N. p., 2016. Web.
Thompson, David N., Apel, William A., Thompson, Vicki S., Reed, David W., & Lacey, Jeffrey A. Thermophilic and thermoacidophilic glycosylation genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods. United States.
Thompson, David N., Apel, William A., Thompson, Vicki S., Reed, David W., and Lacey, Jeffrey A. Tue . "Thermophilic and thermoacidophilic glycosylation genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods". United States.
title = {Thermophilic and thermoacidophilic glycosylation genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods},
author = {Thompson, David N. and Apel, William A. and Thompson, Vicki S. and Reed, David W. and Lacey, Jeffrey A.},
abstractNote = {Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for glycosylating and/or post-translationally modifying proteins using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {1}


Save / Share:

Works referenced in this record:

Deciphering the message in protein sequences: tolerance to amino acid substitutions
journal, March 1990

Powers and Pitfalls in Sequence Analysis: The 70% Hurdle
journal, April 2000

Cloning and transcriptional analysis of the Thermoanaerobacter ethanolicus strain 39E maltose ABC transport system
journal, March 2002

X-ray Structures of the Maltose–Maltodextrin-binding Protein of the Thermoacidophilic Bacterium Alicyclobacillus acidocaldarius Provide Insight into Acid Stability of Proteins
journal, January 2004

  • Schäfer, Karsten; Magnusson, Ulrika; Scheffel, Frank
  • Journal of Molecular Biology, Vol. 335, Issue 1, p. 261-274
  • DOI: 10.1016/j.jmb.2003.10.042

Functional reconstitution of a maltose ATP-binding cassette transporter from the thermoacidophilic gram-positive bacterium Alicyclobacillus acidocaldarius
journal, May 2004

  • Scheffel, Frank; Fleischer, Rebecca; Schneider, Erwin
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1656, Issue 1, p. 57-65
  • DOI: 10.1016/j.bbabio.2004.01.005

Isolation and characterization of a new family 42 β-galactosidase from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius: Identification of the active site residues
journal, February 2008

  • Di Lauro, Barbara; Strazzulli, Andrea; Perugino, Giuseppe
  • Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1784, Issue 2, p. 292-301
  • DOI: 10.1016/j.bbapap.2007.10.013

Thermoacidophilic Microorganisms and their Novel Biocatalysts
journal, December 2004

  • Bertoldo, C.; Dock, C.; Antranikian, G.
  • Engineering in Life Sciences, Vol. 4, Issue 6, p. 521-532
  • DOI: 10.1002/elsc.200402155

Yeast systems for the expression of heterologous gene products
journal, October 1993

Single molecule detection of RNA reporter probes by amplification with Qβ replicase
journal, August 1996

  • Burg, Lawrence J.; Juffras, Ann Marie; Wu, Ye
  • Molecular and Cellular Probes, Vol. 10, Issue 4, p. 257-271
  • DOI: 10.1006/mcpr.1996.0035

Synthesis of an amplifiable reporter RNA for bioassays
journal, January 1986

  • Chu, Barbara C. F.; Kramer, Fred Russell; Orgel, Leslie E.
  • Nucleic Acids Research, Vol. 14, Issue 14, p. 5591-5603
  • DOI: 10.1093/nar/14.14.5591

Current applications of COS cell based transient expression systems
journal, October 1993

Manufacture of Xylose-Based Fermentation Media from Corncobs by Posthydrolysis of Autohydrolysis Liquors
journal, January 2001

  • Garrote, Gil; Domínguez, Herminia; Parajó, Juan C.
  • Applied Biochemistry and Biotechnology, Vol. 95, Issue 3, p. 195-208
  • DOI: 10.1385/ABAB:95:3:195

Ethanol from lignocellulosic biomass techno-economic performance in short-, middle- and long-term
journal, April 2005

  • Hamelinck, Carlo N; Hooijdonk, Geertje van; Faaij, André PC
  • Biomass and Bioenergy, Vol. 28, Issue 4, p. 384-410
  • DOI: 10.1016/j.biombioe.2004.09.002

Immunogenicity and protective efficacy of a tuberculosis DNA vaccine
journal, August 1996

  • Huygen, Kris; Content, Jean; Denis, Olivier
  • Nature Medicine, Vol. 2, Issue 8, p. 893-898
  • DOI: 10.1038/nm0896-893

Biochemistry and genetics of microbial xylanases
journal, June 1996

NASBATM isothermal enzymatic in vitro nucleic acid amplification optimized for the diagnosis of HIV-1 infection
journal, December 1991

  • Kievits, Tim; van Gemen, Bob; van Strijp, Dianne
  • Journal of Virological Methods, Vol. 35, Issue 3, p. 273-286
  • DOI: 10.1016/0166-0934(91)90069-C

Continuous cultures of fused cells secreting antibody of predefined specificity
journal, August 1975

  • Köhler, G.; Milstein, C.
  • Nature, Vol. 256, Issue 5517, p. 495-497
  • DOI: 10.1038/256495a0

The acid hydrolysis of sugarcane bagasse hemicellulose to produce xylose, arabinose, glucose and other products
journal, November 2002

The Effect of Flow Rate of Compressed Hot Water on Xylan, Lignin, and Total Mass Removal from Corn Stover
journal, September 2003

  • Liu, Chaogang; Wyman, Charles E.
  • Industrial & Engineering Chemistry Research, Vol. 42, Issue 21, p. 5409-5416
  • DOI: 10.1021/ie030458k

Baculovirus systems for the expression of human gene products
journal, October 1993

Microbial Cellulose Utilization: Fundamentals and Biotechnology
journal, September 2002

  • Lynd, L. R.; Weimer, P. J.; van Zyl, W. H.
  • Microbiology and Molecular Biology Reviews, Vol. 66, Issue 3, p. 506-577
  • DOI: 10.1128/MMBR.66.3.506-577.2002

Lignocellulose biodegradation: Fundamentals and applications
journal, June 2002

  • Malherbe, S.; Cloete, T. E.
  • Reviews in Environmental Science and Bio/Technology, Vol. 1, Issue 2, p. 105-114
  • DOI: 10.1023/A:1020858910646

Analytical strategies for the use of DNA probes
journal, February 1988

The Synthesis of Bovine Insulin by the Solid Phase Method
journal, November 1966

  • Marglin, B.; Merrifield, R. B.
  • Journal of the American Chemical Society, Vol. 88, Issue 21, p. 5051-5052
  • DOI: 10.1021/ja00973a068

Autocatalytic replication of a recombinant RNA
journal, December 1983

  • Miele, Eleanor A.; Mills, Donald R.; Kramer, Fred Russell
  • Journal of Molecular Biology, Vol. 171, Issue 3, p. 281-295
  • DOI: 10.1016/0022-2836(83)90094-3

Ethanol production from biomass: technology and commercialization status
journal, June 2001

A general method applicable to the search for similarities in the amino acid sequence of two proteins
journal, March 1970

Recent advances in heterologous gene expression in Escherichia coli
journal, October 1993

Prokaryotic glycosylation
journal, February 2001

Strand displacement amplification—an isothermal, in vitro DNA amplification technique
journal, January 1992

  • Walker, G. Terrance; Fraiser, Melinda S.; Schram, James L.
  • Nucleic Acids Research, Vol. 20, Issue 7, p. 1691-1696
  • DOI: 10.1093/nar/20.7.1691

Microbial β-Glucosidases: Cloning, Properties, and Applications
journal, January 2002

  • Bhatia, Yukti; Mishra, Saroj; Bisaria, V. S.
  • Critical Reviews in Biotechnology, Vol. 22, Issue 4, p. 375-407
  • DOI: 10.1080/07388550290789568

Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids
journal, November 1998

Practical limits of function prediction
journal, January 2000

Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different
journal, April 2001

Prediction of protein function from protein sequence and structure
journal, January 1999

  • Whisstock, James C.; Lesk, Arthur M.
  • Quarterly Reviews of Biophysics, Vol. 36, Issue 3, p. 307-340
  • DOI: 10.1017/S0033583503003901

Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine
journal, September 1999

  • Witkowski, Andrzej; Joshi, Anil K.; Lindqvist, Ylva
  • Biochemistry, Vol. 38, Issue 36, p. 11643-11650
  • DOI: 10.1021/bi990993h

Xylanases, xylanase families and extremophilic xylanases
journal, January 2005

Purification and Properties of Acid Stable Xylanases from Aspergillus kawachii
journal, January 1992

  • Ito, Kiyoshi; Ogasawara, Hironobu; Sugimoto, Takashi
  • Bioscience, Biotechnology, and Biochemistry, Vol. 56, Issue 4, p. 547-550
  • DOI: 10.1271/bbb.56.547

Microbial hemicellulases
journal, June 2003

Gene cloning, sequencing, and characterization of a family 9 endoglucanase (CelA) with an unusual pattern of activity from the thermoacidophile Alicyclobacillus acidocaldarius ATCC27009
journal, December 2002

  • K., Eckert; F., Zielinski; Leggio, L. Lo
  • Applied Microbiology and Biotechnology, Vol. 60, Issue 4, p. 428-436
  • DOI: 10.1007/s00253-002-1131-4

A new class of biodegradable materials: Poly-3-hydroxy-butyrate/steam exploded straw fiber composites. I. Thermal and impact behavior
journal, September 1993

  • Avella, Maurizio; Martuscelli, Ezio; Pascucci, Beniamino
  • Journal of Applied Polymer Science, Vol. 49, Issue 12, p. 2091-2103
  • DOI: 10.1002/app.1993.070491205

Interlaboratory testing of methods for assay of xylanase activity
journal, May 1992

  • Bailey, Michael J.; Biely, Peter; Poutanen, Kaisa
  • Journal of Biotechnology, Vol. 23, Issue 3, p. 257-270
  • DOI: 10.1016/0168-1656(92)90074-J

Molecular diversity of thermophilic cellulolytic and hemicellulolytic bacteria
journal, February 1999

Glycosidases and glycosyl transferases in glycoside and oligosaccharide synthesis
journal, February 1998

The nature of lignocellulosics and their pretreatments for enzymatic hydrolysis
book, January 1982

  • Fan, L. T.; Lee, Young-Hyun; Gharpuray, M. M.
  • Microbial Reactions, p. 157-187
  • DOI: 10.1007/3540116982_4

Development of gas phase bioreactors for the removal of nitrogen oxides from synthetic flue gas streams
journal, October 2002

Crystallographic and mutational analyses of an extremely acidophilic and acid-stable xylanase: biased distribution of acidic residues and importance of Asp37 for catalysis at low pH
journal, December 1998

  • Fushinobu, S.; Ito, K.; Konno, M.
  • Protein Engineering Design and Selection, Vol. 11, Issue 12, p. 1121-1128
  • DOI: 10.1093/protein/11.12.1121

Transformation of Acidiphilium by electroporation and conjugation
journal, May 1992

  • Glenn, Anne W.; Roberto, Frank F.; Ward, Thomas E.
  • Canadian Journal of Microbiology, Vol. 38, Issue 5, p. 387-393
  • DOI: 10.1139/m92-065

Pretreatment for enhanced hydrolysis of cellulosic biomass
journal, January 1984

Comparison of the economics of acid and enzymatic hydrolysis of newsprint
journal, April 1978

  • Grethlein, Hans E.
  • Biotechnology and Bioengineering, Vol. 20, Issue 4, p. 503-525
  • DOI: 10.1002/bit.260200404

journal, February 1982

Fungal Upgrading of Wheat Straw for Straw-Thermoplastics Production
journal, January 2004

  • Houghton, Tracy P.; Thompson, David N.; Hess, J. Richard
  • Applied Biochemistry and Biotechnology, Vol. 113, Issue 1-3, p. 71-93
  • DOI: 10.1385/ABAB:113:1-3:071

Microbial Pretreatment of Biomass: Potential for Reducing Severity of Thermochemical Biomass Pretreatment
journal, January 2003

  • Keller, Fred A.; Hamilton, Jenny E.; Nguyen, Quang A.
  • Applied Biochemistry and Biotechnology, Vol. 105, Issue 1-3, p. 27-42
  • DOI: 10.1385/ABAB:105:1-3:27

journal, October 2003

  • Kenealy, William R.; Jeffries, Thomas W.
  • Biotechnology Letters, Vol. 25, Issue 19, p. 1619-1623
  • DOI: 10.1023/A:1025668031928

Partial acid hydrolysis of cellulosic materials as a pretreatment for enzymatic hydrolysis
journal, July 1980

  • Knappert, Diane; Grethlein, Hans; Converse, Alvin
  • Biotechnology and Bioengineering, Vol. 22, Issue 7, p. 1449-1463
  • DOI: 10.1002/bit.260220711

Molecular and biotechnological aspects of xylanases
journal, July 1999

Characterization of a β-glycosidase from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius
journal, April 2006

Oxygen effects on thermophilic microbial populations in biofilters treating nitric oxide containing off-gas streams
journal, October 2001

  • Lee, Brady D.; Apel, William A.; Smith, William A.
  • Environmental Progress, Vol. 20, Issue 3, p. 157-166
  • DOI: 10.1002/ep.670200311

Overview and Evaluation of Fuel Ethanol from Cellulosic Biomass: Technology, Economics, the Environment, and Policy
journal, November 1996

Specificity of the protein secretory apparatus: secretion of the heat-labile enterotoxin B subunit pentamers by different species of Gram- bacteria
journal, January 1995

Industrial Scale-Up of pH-Controlled Liquid Hot Water Pretreatment of Corn Fiber for Fuel Ethanol Production
journal, January 2005

  • Mosier, Nathan S.; Hendrickson, Richard; Brewer, Mark
  • Applied Biochemistry and Biotechnology, Vol. 125, Issue 2, p. 77-98
  • DOI: 10.1385/ABAB:125:2:077

Purification and characterization of an acidophilic xylanase from Aureobasidium pullulans var. melanigenum and sequence analysis of the encoding gene
journal, January 2001

  • Ohta, Kazuyoshi; Moriyama, Satoshi; Tanaka, Hidenori
  • Journal of Bioscience and Bioengineering, Vol. 92, Issue 3, p. 262-270
  • DOI: 10.1016/S1389-1723(01)80260-7

Simultaneous saccharification and fermentation of cellulose: Effect of ethanol on enzymatic saccharification of cellulose
journal, April 1985

  • Ooshima, Hiroshi; Ishitani, Yohsuke; Harano, Yoshio
  • Biotechnology and Bioengineering, Vol. 27, Issue 4, p. 389-397
  • DOI: 10.1002/bit.260270402

Biotransformation of Toxic Organic and Inorganic Contaminants by Halophilic Bacteria
book, January 2004

The Path Forward for Biofuels and Biomaterials
journal, January 2006

  • Ragauskas, Arthur J.; Williams, Charlotte K.; Davison, Brian H.
  • Science, Vol. 311, Issue 5760, p. 484-489
  • DOI: 10.1126/science.1114736

Biomechanical and Biochemical Pulping of Sugarcane Bagasse with Ceriporiopsis subvermispora Fungal and Xylanase Pretreatments
journal, March 2001

  • Ramos, Juan; González, Mercedes; Ramírez, Felipe
  • Journal of Agricultural and Food Chemistry, Vol. 49, Issue 3, p. 1180-1186
  • DOI: 10.1021/jf0011586

Quantitative Saccharification of Wood and Cellulose
journal, January 1945

  • Saeman, Jerome F.; Bubl, Janet L.; Harris, Elwin E.
  • Industrial & Engineering Chemistry Analytical Edition, Vol. 17, Issue 1, p. 35-37
  • DOI: 10.1021/i560137a008

Dilute Acid Pretreatment, Enzymatic Saccharification, and Fermentation of Rice Hulls to Ethanol
journal, January 2005

  • Saha, Badal C.; Iten, Loren B.; Cotta, Michael A.
  • Biotechnology Progress, Vol. 21, Issue 3, p. 816-822
  • DOI: 10.1021/bp049564n

Rapid production of thermostable cellulase-free xylanase by a strain of Bacillus subtilis and its properties
journal, June 2002

  • Sá-Pereira, Paula; Mesquita, Alexandra; Duarte, José C.
  • Enzyme and Microbial Technology, Vol. 30, Issue 7, p. 924-933
  • DOI: 10.1016/S0141-0229(02)00034-0

Dilute-Sulfuric Acid Pretreatment of Corn Stover in Pilot-Scale Reactor
book, January 2003

An extremely thermostable xylanase from the thermophilic eubacterium Thermotoga
journal, July 1991

  • Simpson, H D; Haufler, U R; Daniel, R M
  • Biochemical Journal, Vol. 277, Issue 2, p. 413-417
  • DOI: 10.1042/bj2770413

Cellulase-free xylanases from Bacillus and other microorganisms
journal, February 2000

Glycosyl hydrolases from hyperthermophiles
journal, February 1997

  • Sunna, Anwar; Moracci, Marco; Rossi, Mose
  • Extremophiles, Vol. 1, Issue 1, p. 2-13
  • DOI: 10.1007/s007920050009

Production of a cellulase-free xylanase from agricultural waste materials by a thermotolerant Streptomyces sp.
journal, October 2001

  • Techapun, Charin; Sinsuwongwat, Suphawat; Poosaran, Naiyatat
  • Biotechnology Letters, Vol. 23, Issue 20, p. 1685-1689
  • DOI: 10.1023/A:1012448229555

Comparison of pretreatment methods on the basis of available surface area
journal, January 1992

  • Thompson, David N.; Chen, Hsin-Chih; Grethlein, Hans E.
  • Bioresource Technology, Vol. 39, Issue 2, p. 155-163
  • DOI: 10.1016/0960-8524(92)90135-K

In vitro degradation of natural insoluble lignin in aqueous media by the extracellular peroxidases of Phanerochaete chrysosporium
journal, March 1998

Measurement of fumonisins in corn with a fiber optic fluoroimmunosensor
conference, May 1997

  • Thompson, Vicki S.; Maragos, Chris M.; Thompson, Richard B.
  • Advances in Fluorescence Sensing Technology III, Vol. 2980
  • DOI: 10.1117/12.273560

Preliminary Investigation of Fungal Bioprocessing of Wheat Straw for Production of Straw-Thermoplastic Composites
book, January 2003

  • Thompson, David N.; Houghton, Tracy P.; Lacey, Jeffrey A.
  • Biotechnology for Fuels and Chemicals, p. 423-436
  • DOI: 10.1007/978-1-4612-0057-4_35

Purification and Characterization of a Novel Thermo-Alkali-Stable Catalase from Thermus brockianus
journal, January 2003

  • Thompson, Vicki S.; Schaller, Kastli D.; Apel, William A.
  • Biotechnology Progress, Vol. 19, Issue 4, p. 1292-1299
  • DOI: 10.1021/bp034040t

Potential and utilization of thermophiles and thermostable enzymes in biorefining
journal, January 2007

  • Turner, Pernilla; Mamo, Gashaw; Karlsson, Eva
  • Microbial Cell Factories, Vol. 6, Issue 1, 9
  • DOI: 10.1186/1475-2859-6-9

The first description of an archaeal hemicellulase: the xylanase from Thermococcus zilligii strain AN1
journal, November 1999

Xylanases in bleaching: From an idea to the industry
journal, March 1994

Characterization of a new bacteriophage which infects bacteria of the genus Acidiphilium
journal, November 1993

Electrotransformation of Acidophilic, Heterotrophic, Gram-negative Bacteria
book, January 2000

PCR ligation mutagenesis in transformable streptococci: application and efficiency
journal, April 2002

  • Lau, Peter C. Y.; Sung, Chang Kyoo; Lee, Janet H.
  • Journal of Microbiological Methods, Vol. 49, Issue 2, p. 193-205
  • DOI: 10.1016/S0167-7012(01)00369-4

Purification, Properties and Structural Aspects of a Thermoacidophilic alpha-Amylase from Alicyclobacillus Acidocaldarius Atcc 27009
journal, December 1994

Bacterial glycoproteins: Functions, biosynthesis and applications
journal, April 2003

  • Upreti, Raj K.; Kumar, Manoj; Shankar, V.
  • PROTEOMICS, Vol. 3, Issue 4, p. 363-379
  • DOI: 10.1002/pmic.200390052