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Title: Controlling Lipid Micelle Stability Using Oligonucleotide Headgroups

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Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
Report Number(s):
Journal ID: ISSN 0002-7863
DOE Contract Number:
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of the American Chemical Society; Journal Volume: 137; Journal Issue: 6
Country of Publication:
United States

Citation Formats

Wilner, S, Sparks, S, Cowburn, D, Girvin, M, and Levy, M. Controlling Lipid Micelle Stability Using Oligonucleotide Headgroups. United States: N. p., 2015. Web. doi:10.1021/ja512012m.
Wilner, S, Sparks, S, Cowburn, D, Girvin, M, & Levy, M. Controlling Lipid Micelle Stability Using Oligonucleotide Headgroups. United States. doi:10.1021/ja512012m.
Wilner, S, Sparks, S, Cowburn, D, Girvin, M, and Levy, M. 2015. "Controlling Lipid Micelle Stability Using Oligonucleotide Headgroups". United States. doi:10.1021/ja512012m.
title = {Controlling Lipid Micelle Stability Using Oligonucleotide Headgroups},
author = {Wilner, S and Sparks, S and Cowburn, D and Girvin, M and Levy, M},
abstractNote = {},
doi = {10.1021/ja512012m},
journal = {Journal of the American Chemical Society},
number = 6,
volume = 137,
place = {United States},
year = 2015,
month = 1
  • Nanolipoprotein particles (NLPs) consist of a discoidal phospholipid lipid bilayer confined by an apolipoprotein belt. NLPs are a promising platform for a variety of biomedical applications due to their biocompatibility, size, definable composition, and amphipathic characteristics. However, poor serum stability hampers the use of NLPs for in vivo applications such as drug formulation. In this study, NLP stability was enhanced upon the incorporation and subsequent UV-mediated intermolecular cross-linking of photoactive DiynePC phospholipids in the lipid bilayer, forming cross-linked nanoparticles (X-NLPs). Both the concentration of DiynePC in the bilayer and UV exposure time significantly affected the resulting X-NLP stability in 100%more » serum, as assessed by size exclusion chromatography (SEC) of fluorescently labeled particles. Cross-linking did not significantly impact the size of X-NLPs as determined by dynamic light scattering and SEC. X-NLPs had essentially no degradation over 48 h in 100% serum, which is a drastic improvement compared to non-cross-linked NLPs (50% degradation by ~10 min). X-NLPs had greater uptake into the human ATCC 5637 bladder cancer cell line compared to non-cross-linked particles, indicating their potential utility for targeted drug delivery. X-NLPs also exhibited enhanced stability following intravenous administration in mice. Lastly, these results collectively support the potential utility of X-NLPs for a variety of in vivo applications.« less
  • In recent years, substantial progress has been made in the theoretical understanding of micelle formation through the use of semiempirical expressions for the free energies, and through a detailed analysis of the aggregation process. Certain aspects of these treatments also have implications for an understanding of the conditions under which a liquid crystalline structure is more stable than a micellar solution. The purpose of the present work is to point out some general relations between the micellar solutions and different liquid crystalline structures and to show that these rationalize some properties of the phase diagrams of water-amphiphile systems. 14 references.
  • A simple and rapid strategy for distinguishing between positively hybridizing colonies and false positive-hybridization signals is described. The isolation of a specific DNA sequence depends on the ability to distinguish between a clone that contains the correct sequence and a false hybridization-positive or background signal. This procedure utilizes the same /sup 32/P-labeled oligonucleotide mixture both as a screening probe and as a sequencing primer. The mixture of oligonucleotides is used as a primer to obtain sequence information directly from double-stranded DNA. Conditions for sequencing with oligonucleotides having up to 64-fold degeneracy are described. Since the sequence information obtained is directlymore » adjacent to the site of oligonucleotide: DNA hybridization, it is necessary to know only a minimal length of DNA or peptide sequence to both design oligonucleotide probes and confirm the authenticity of the hybridization positives. The advantages of the degenerate oligonucleotide sequencing method include the rapid, reliable identification of authentic versus false hybridization positives made directly without subcloning into single-stranded M13 phage, without sequencing large regions of DNA, or without synthesizing sequence-specific primers.« less
  • We have developed a high-density DNA probe array and accompanying biochemical and informatic methods to order clones from genomic libraries. This approach involves a series of enzymatic steps for capturing a set of short dispersed sequence markers scattered throughout a high-molecular-weight DNA. By this process, all the ambiguous sequences lying adjacent to a given Type IIS restriction site are ligated between two DNA adaptors. These markers, once amplified and labeled by PCR, can be hybridized and detected on a high-density olligonucleotide array bearing probes complementary to all possible markers. The array is synthesized using light-directed combinatorial chemistry. For each clonemore » in a genomic library, a characteristic set of sequence markers can be determined. On the basis of the similarity between the marker sets for each pair of clones, their relative overlap can be measured. The library can be sequentially ordered into a contig map using this overlap information. This new methodology does not require gel-based methods or prior sequence information and involves manipulations that should allow for easy adaptation to automated processing and data collection. 28 refs., 9 figs., 2 tabs.« less
  • No abstract prepared.