A New Metal Binding Domain Involved in Cadmium, Cobalt and Zinc Transport

Smith, Aaron T.; Barupala, Dulmini; Stemmler, Timothy L.; Rosenzweig, Amy C.

doi:10.1038/nchembio.1863

Title: A New Metal Binding Domain Involved in Cadmium, Cobalt and Zinc Transport

Journal Article · Mon Jul 20 00:00:00 EDT 2015 · Nature Chemical Biology

DOI:https://doi.org/10.1038/nchembio.1863· OSTI ID:1228887

Smith, Aaron T. ^[1]; Barupala, Dulmini ^[2]; Stemmler, Timothy L. ^[2]; Rosenzweig, Amy C. ^[1]

Northwestern Univ., Evanston, IL (United States)
Wayne State Univ., Detroit, MI (United States)

In the P1B-ATPases, which couple cation transport across membranes to ATP hydrolysis, are central to metal homeostasis in all organisms. An important feature of P1B-ATPases is the presence of soluble metal binding domains (MBDs) that regulate transport activity. Only one type of MBD has been characterized extensively, but bioinformatics analyses indicate that a diversity of MBDs may exist in nature. Here we report the biochemical, structural and functional characterization of a new MBD from the Cupriavidus metallidurans P_1B-4-ATPase CzcP (CzcP MBD). The CzcP MBD binds two Cd²⁺, Co²⁺ or Zn²⁺ ions in distinct and unique sites and adopts an unexpected fold consisting of two fused ferredoxin-like domains. Both in vitro and in vivo activity assays using full-length CzcP, truncated CzcP and several variants indicate a regulatory role for the MBD and distinct functions for the two metal binding sites. Moreover, these findings elucidate a previously unknown MBD and suggest new regulatory mechanisms for metal transport by P_1B-ATPases.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

DOE Contract Number:: SC00112704

OSTI ID:: 1228887

Report Number(s):: BNL-110962-2015-JA

Journal Information:: Nature Chemical Biology, Vol. 11; ISSN 1552-4450

Publisher:: Nature Publishing Group

Country of Publication:: United States

Language:: English

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