Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

Extraction of solubles from plant biomass for use as microbial growth stimulant and methods related thereto

Patent ·
OSTI ID:1228372
A method for producing a microbial growth stimulant (MGS) from a plant biomass is described. In one embodiment, an ammonium hydroxide solution is used to extract a solution of proteins and ammonia from the biomass. Some of the proteins and ammonia are separated from the extracted solution to provide the MGS solution. The removed ammonia can be recycled and the proteins are useful as animal feeds. In one embodiment, the method comprises extracting solubles from pretreated lignocellulosic biomass with a cellulase enzyme-producing growth medium (such T. reesei) in the presence of water and an aqueous extract.
Research Organization:
BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY, East Lansing, MI (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FC02-07ER64494;
Assignee:
BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY
Patent Number(s):
9,206,446
Application Number:
12/763,102
OSTI ID:
1228372
Country of Publication:
United States
Language:
English

References (122)

Increasing nutrient availability of feather meal for ruminants and non-ruminants using an ammonia pressurisation/depressurisation process journal May 1999
Assays for Total Protein book September 1995
Iogen's Demonstration Process for Producing Ethanol from Cellulosic Biomass book December 2005
Toward an aggregated understanding of enzymatic hydrolysis of cellulose: Noncomplexed cellulase systems journal November 2004
Effect of particle size based separation of milled corn stover on AFEX pretreatment and enzymatic digestibility journal January 2006
Increasing cellulose accessibility is more important than removing lignin: A comparison of cellulose solvent-based lignocellulose fractionation and soaking in aqueous ammonia journal September 2010
Cocurrent downflow circulating fluidized bed (downer) reactors - A state of the art review journal October 1995
Development of a pilot process for the production of alfalfa peptide isolate: Production of alfalfa peptide isolate
  • Prévot-D'Alvise, Nathalie; Lesueur-Lambert, Christine; Fertin-Bazus, Anne
  • Journal of Chemical Technology & Biotechnology, Vol. 78, Issue 5 https://doi.org/10.1002/jctb.824
journal April 2003
Production of Cellulase by Solid-State Fermentation book January 1999
Production of Cellulase in Solid-State Fermentation with Trichoderma reesei MCG 80 on Wheat Straw book January 1996
Pretreatment of Switchgrass by Ammonia Fiber Explosion (AFEX) book December 2004
Separation of Glucose and Pentose Sugars by Selective Enzyme Hydrolysis of AFEX-Treated Corn Fiber book January 2007
Lignocellulosic Biomass Pretreatment Using AFEX book January 2009
Variability of laccase activity in the white-rot basidiomycetePleurotus ostreatus journal August 2002
Cost estimates and sensitivity analyses for the ammonia fiber explosion process journal March 1998
The ammonia freeze explosion (AFEX) process: A practical lignocellulose pretreatment journal March 1991
Integrated production of ethanol fuel and protein from Coastal Bermudagrass journal March 1994
Biotechnological applications and potential of wood-degrading mushrooms of the genus Pleurotus journal April 2002
Biorefinery journal October 2003
Principles of biorefineries journal April 2004
Biotechnological processes for conversion of corn into ethanol journal December 2004
Ethanol fermentation from biomass resources: current state and prospects journal December 2005
Optimization of enzymatic hydrolysis and ethanol fermentation from AFEX-treated rice straw journal April 2009
Effects of the pelleting conditions on chemical composition and sugar yield of corn stover, big bluestem, wheat straw, and sorghum stalk pellets journal October 2011
Comparative study on the growth and yield of Pleurotus ostreatus mushroom on different lignocellulosic by-products journal February 2003
Hemicellulose bioconversion journal May 2003
Mushroom spent straw: a potential substrate for an ethanol-based biorefinery journal January 2008
Improving Accessibility and Reactivity of Celluloses of Annual Plants for the Synthesis of Methylcellulose journal October 2005
Acid–base interactions and swelling of cellulose fibers in organic liquids journal July 2005
Body temperature-responsive gels derived from hydroxypropylcellulose bearing lignin II: adsorption and release behavior journal May 2006
Splitting tendency of cellulosic fibers. Part 2: Effects of fiber swelling in alkali solutions journal March 2006
Detoxification methods of aflatoxins. A review journal May 1995
Botanical fractions of rice straw colonized by white-rot fungi: changes in chemical composition and structure journal October 1995
Production and characterization of cellulolytic enzymes from Trichoderma reesei grown on various carbon sources journal January 1992
Switchgrass as a sustainable bioenergy crop journal April 1996
Replacement of fish meal in diets for Australian silver perch, Bidyanus bidyanus: I. Digestibility of alternative ingredients journal June 2000
Fuel ethanol after 25 years journal December 1999
Nutritional potential and functional properties of sweet and bitter lupin seed protein isolates journal September 2001
Pretreatment of Miscanthus for hydrogen production by Thermotoga elfii journal November 2002
The effect of different treatment conditions on biomass binder preparation for lignite briquette journal November 2001
Biological waste air treatment in biofilters journal June 1997
Obtaining a protein concentrate from integral defatted sunflower flour journal June 2001
An initial assessment of spent mushroom compost as a potential energy feedstock journal September 2001
Oyster mushroom cultivation with rice and wheat straw journal May 2002
Hydrolysis of lignocellulosic materials for ethanol production: a review journal May 2002
Assessment of the influence of media particle size on the biofiltration of odorous exhaust ventilation air from a piggery facility journal September 2002
Composting of a crop residue through treatment with microorganisms and subsequent vermicomposting journal November 2002
Induction and catabolite repression mechanisms of cellulase in fungi journal January 2001
A quantitative review comparing the yields of two candidate C4 perennial biomass crops in relation to nitrogen, temperature and water journal July 2004
Features of promising technologies for pretreatment of lignocellulosic biomass journal April 2005
Coordinated development of leading biomass pretreatment technologies journal December 2005
Combined sugar yields for dilute sulfuric acid pretreatment of corn stover followed by enzymatic hydrolysis of the remaining solids journal December 2005
Partial flow of compressed-hot water through corn stover to enhance hemicellulose sugar recovery and enzymatic digestibility of cellulose journal December 2005
Optimization of pH controlled liquid hot water pretreatment of corn stover journal December 2005
Lime pretreatment and enzymatic hydrolysis of corn stover journal December 2005
Pretreatment and fractionation of corn stover by ammonia recycle percolation process journal December 2005
Optimization of the ammonia fiber explosion (AFEX) treatment parameters for enzymatic hydrolysis of corn stover journal December 2005
Process and economic analysis of pretreatment technologies journal December 2005
Comparative sugar recovery data from laboratory scale application of leading pretreatment technologies to corn stover journal December 2005
Recent process improvements for the ammonia fiber expansion (AFEX) process and resulting reductions in minimum ethanol selling price journal November 2008
Mixture optimization of six core glycosyl hydrolases for maximizing saccharification of ammonia fiber expansion (AFEX) pretreated corn stover journal April 2010
Two-step SSCF to convert AFEX-treated switchgrass to ethanol using commercial enzymes and Saccharomyces cerevisiae 424A(LNH-ST) journal November 2010
Evaluating the impact of ammonia fiber expansion (AFEX) pretreatment conditions on the cost of ethanol production journal January 2011
Combined effect of pelleting and pretreatment on enzymatic hydrolysis of switchgrass journal July 2012
Does densification influence the steam pretreatment and enzymatic hydrolysis of softwoods to sugars? journal October 2012
Bioethanol journal April 2006
Advancement and comparative profiles in the production technologies using solid-state and submerged fermentation for microbial cellulases journal June 2010
Cellulase production using biomass feed stock and its application in lignocellulose saccharification for bio-ethanol production journal February 2009
Bio-ethanol – the fuel of tomorrow from the residues of today journal December 2006
In vitro and in vivo digestibility of soya-bean straw treated with various alkalis journal August 1990
A Transition from Cellulose Swelling to Cellulose Dissolution by o -Phosphoric Acid:  Evidence from Enzymatic Hydrolysis and Supramolecular Structure journal February 2006
Cooperative and Competitive Binding in Synergistic Mixtures of Thermobifida fusca Cellulases Cel5A, Cel6B, and Cel9A journal August 2002
Biodegradation of Viticulture Wastes by Pleurotus :  A Source of Microbial and Human Food and Its Potential Use in Animal Feeding journal April 2002
Investigation and Optimization of the Factors Influencing Sorghum Protein Extraction journal November 2003
Extractability and solubility of leaf protein journal January 1973
Protein extraction from Atriplex lampa leaves: Potential use as forage for animals used for human diets journal September 1999
Miscanthus for Renewable Energy Generation: European Union Experience and Projections for Illinois journal October 2004
Growth of the plant cell wall journal November 2005
Multi-scale visualization and characterization of lignocellulosic plant cell wall deconstruction during thermochemical pretreatment journal January 2011
A novel integrated biological process for cellulosic ethanol production featuring high ethanol productivity, enzyme recycling and yeast cells reuse journal January 2012
Liquid-vapor interfacial properties of water-ammonia mixtures: Dependence on ammonia concentration journal November 2005
Cellulosic ethanol production from AFEX-treated corn stover using Saccharomyces cerevisiae 424A(LNH-ST) journal January 2009
Bio-technologies of Recycling Agro-industrial Wastes for the Production of Commercially Important Fungal Polysaccharides and Mushrooms journal December 2003
Leaf photosynthesis in the C 4 -grass Miscanthus x giganteus , growing in the cool temperate climate of southern England journal January 1996
Pilot Plant Production of an Edible Alfalfa Protein Concentrate journal September 1981
Toward a Systems Approach to Understanding Plant Cell Walls journal December 2004
The Path Forward for Biofuels and Biomaterials journal January 2006
Gene Integration and Expression and Extracellular Secretion of Erwinia chrysanthemi Endoglucanase CelY (celY) and CelZ (celZ) in Ethanologenic Klebsiella oxytoca P2 journal January 2001
Microbial Cellulose Utilization: Fundamentals and Biotechnology journal September 2002
The impacts of pretreatment on the fermentability of pretreated lignocellulosic biomass: a comparative evaluation between ammonia fiber expansion and dilute acid pretreatment journal January 2009
Comparing the fermentation performance of Escherichia coli KO11, Saccharomyces cerevisiae 424A(LNH-ST) and Zymomonas mobilis AX101 for cellulosic ethanol production journal January 2010
Evaluation of pretreatment with Pleurotus ostreatus for enzymatic hydrolysis of rice straw journal December 2005
Biomass Densification – Cubing Operations and Costs for corn Stover journal January 2004
Economics of Producing fuel Pellets from Biomass journal January 2006
Roll Press Briquetting and Pelleting of Corn Stover and Switchgrass journal January 2009
Enzymatic Hydrolysis of Ammonia-Treated Rice Straw journal January 2003
Microbial Pretreatment of Biomass: Potential for Reducing Severity of Thermochemical Biomass Pretreatment journal January 2003
Fungal Upgrading of Wheat Straw for Straw-Thermoplastics Production journal January 2004
Enhancing Profitability of Dry Mill Ethanol Plants: Process Modeling and Economics of Conversion of Degermed Defibered Corn to Ethanol journal January 2005
Leaf Protein from Ammonia-Treated Dwarf Elephant Grass (Pennisetum purpureum Schum cv. Mott) journal January 2005
Enzyme Recovery and Recycling Following Hydrolysis of Ammonia Fiber Explosion–Treated Corn Stover journal January 2005
Understanding Factors that Limit Enzymatic Hydrolysis of Biomass: Characterization of Pretreated Corn Stover journal January 2005
The Future of Coproducts From Corn Processing journal January 2006
Enhancement of the Enzymatic Digestibility of Waste Newspaper Using Tween journal January 2006
Pretreatment of Corn Stover by Low-Liquid Ammonia Recycle Percolation Process journal January 2006
Element Concentrations of Dry-Grind Corn-Processing Streams journal January 2006
Extrusion Processing for Ammonia Fiber Explosion (AFEX) journal January 1999
Solid-State Fermentation of Lignocellulosic Plant Residues from Brassica napus by Pleurotus ostreatus journal January 1999
Optimizing Ammonia Pressurization/Depressurization Processing Conditions to Enhance Enzymatic Susceptibility of Dwarf Elephant Grass journal January 2000
Enzymatic Hydrolysis of Ammonia-Treated Sugar Beet Pulp journal January 2001
Predicting Digestibility of Ammonia Fiber Explosion (AFEX)-Treated Rice Straw journal January 2002
Cellulase Adsorption and an Evaluation of Enzyme Recycle During Hydrolysis of Steam-Exploded Softwood Residues journal January 2002
The World Mushroom Industry: Trends and Technological Development journal January 2006
Performance of 15 Genotypes at Five Sites in Europe journal January 2001
Cutting Frequency and Nitrogen Fertilization Effects on Yield and Nitrogen Concentration of Switchgrass in a Short Season Area journal January 1999
Building a Bridge to the Ethanol Industry--Follow-up Project: Period of Performance; February 22, 2001- December 31, 2002 report March 2003
Technical and Financial Feasibility Analysis of Distributed Bioprocessing Using Regional Biomass Pre-Processing Centers journal January 2007
Improving Digestibility of Straws for Ruminant Feed by Aqueous Ammonia journal July 1972
Complete Rations for Dairy Cattle. II. Sugarcane Bagasse Pellets as Roughage in Blended Rations for Lactating Cows journal June 1975
Complete Rations for Dairy Cattle. V. Interaction of Sugarcane Bagasse Quantity and Form with Soybean Meal, Urea, and Starea journal September 1975
Complete Rations for Growing Dairy Replacements Utilizing By-Product Feedstuffs journal September 1980
Biomass Feedstock Pre-Processing – Part 1: Pre-Treatment book July 2011