High-stringency screening of target-binding partners using a microfluidic device

Soh, Hyongsok; Lou, Xinhui; Lagally, Eric

High-stringency screening of target-binding partners using a microfluidic device

Patent · 01 December 2015

OSTI ID:1227363

Soh, Hyongsok; Lou, Xinhui; Lagally, Eric

The invention provides a method of screening a library of candidate agents by contacting the library with a target in a reaction mixture under a condition of high stringency, wherein the target includes a tag that responds to a controllable force applied to the tag, and passing the members of the library through a microfluidic device in a manner that exposes the library members to the controllable force, thereby displacing members of the library that are bound to the target relative to their unbound counterparts. Kits and systems for use with the methods of the invention are also provided.

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Research Organization:: The Regents of the University of California , Oakland, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: W-7405-ENG-48

Assignee:: The Regents of the University of California

Patent Number(s):: 9,200,382

Application Number:: 12/286,965

OSTI ID:: 1227363

Country of Publication:: United States

Language:: English

References (51)

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