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Title: Microfluidic devices, systems, and methods for quantifying particles using centrifugal force

Abstract

Embodiments of the present invention are directed toward microfluidic systems, apparatus, and methods for measuring a quantity of cells in a fluid. Examples include a differential white blood cell measurement using a centrifugal microfluidic system. A method may include introducing a fluid sample containing a quantity of cells into a microfluidic channel defined in part by a substrate. The quantity of cells may be transported toward a detection region defined in part by the substrate, wherein the detection region contains a density media, and wherein the density media has a density lower than a density of the cells and higher than a density of the fluid sample. The substrate may be spun such that at least a portion of the quantity of cells are transported through the density media. Signals may be detected from label moieties affixed to the cells.

Inventors:
; ;
Publication Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1226547
Patent Number(s):
9,186,668
Application Number:
12/891,956
Assignee:
Sandia Corporation (Albuquerque, NM) SNL-A
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Resource Relation:
Patent File Date: 2010 Sep 28
Country of Publication:
United States
Language:
English
Subject:
72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS

Citation Formats

Schaff, Ulrich Y., Sommer, Gregory J., and Singh, Anup K.. Microfluidic devices, systems, and methods for quantifying particles using centrifugal force. United States: N. p., 2015. Web.
Schaff, Ulrich Y., Sommer, Gregory J., & Singh, Anup K.. Microfluidic devices, systems, and methods for quantifying particles using centrifugal force. United States.
Schaff, Ulrich Y., Sommer, Gregory J., and Singh, Anup K.. Tue . "Microfluidic devices, systems, and methods for quantifying particles using centrifugal force". United States. doi:. https://www.osti.gov/servlets/purl/1226547.
@article{osti_1226547,
title = {Microfluidic devices, systems, and methods for quantifying particles using centrifugal force},
author = {Schaff, Ulrich Y. and Sommer, Gregory J. and Singh, Anup K.},
abstractNote = {Embodiments of the present invention are directed toward microfluidic systems, apparatus, and methods for measuring a quantity of cells in a fluid. Examples include a differential white blood cell measurement using a centrifugal microfluidic system. A method may include introducing a fluid sample containing a quantity of cells into a microfluidic channel defined in part by a substrate. The quantity of cells may be transported toward a detection region defined in part by the substrate, wherein the detection region contains a density media, and wherein the density media has a density lower than a density of the cells and higher than a density of the fluid sample. The substrate may be spun such that at least a portion of the quantity of cells are transported through the density media. Signals may be detected from label moieties affixed to the cells.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Nov 17 00:00:00 EST 2015},
month = {Tue Nov 17 00:00:00 EST 2015}
}

Patent:

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