Comparison of immunoassay field tests and laboratory results for PCB, PAH, BTEX, and mercury contaminated soils

Title: Comparison of immunoassay field tests and laboratory results for PCB, PAH, BTEX, and mercury contaminated soils

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Abstract

Immunoassay tests were used as in situ field screening tools for simultaneous assessment and remediation of soil contaminated with mercury and organics (polychlorinated biphenyls (PCB), polyaromatic hydrocarbons (PAH), and BTEX). Soil samples from approximately 200 sites including metering and compressor stations were investigated along gas pipelines. The suspected contamination originated from formerly used mercury manometers and pipeline liquids. An enzyme-linked immunosorbent assay (ELISA) was used for the detection of organic and mercury contaminants. ELISA combines selective antibodies with sensitive enzyme reactions to produce semiquantitative analytical systems capable of detecting very low levels of chemicals. Color sample tubes were compared to the color of standard tubes to semiquantitate the amount of mercury, PCB, PAH, and BBTEX present in the samples. Two standards were tested in order to eliminate the effects of false negatives (e.g., contaminant not detected when present) or false positives (e.g., contamination detected when not present). For verification purposes, selected samples that were determined to be below action level with the immunoassay tests were sent to the laboratory.

Authors:

Hammes, U ^[1]

IT Corp., Houston, TX (United States)

Publication Date:: 1995-09-01

OSTI Identifier:: 122445

Report Number(s):: CONF-9510211-
Journal ID: GRWAAP; ISSN 0017-467X; TRN: IM9547%%25

Resource Type:: Journal Article

Journal Name:: Ground Water

Additional Journal Information:: Journal Volume: 33; Journal Issue: 5; Conference: 1995 Association of Ground Water Scientists & Engineers (AGWSE) educational program, Indianapolis, IN (United States), 28-30 Oct 1995; Other Information: PBD: Sep-Oct 1995

Country of Publication:: United States

Language:: English

Subject:: 54 ENVIRONMENTAL SCIENCES; POLYCHLORINATED BIPHENYLS; ENZYME IMMUNOASSAY; POLYCYCLIC AROMATIC HYDROCARBONS; BENZENE; TOLUENE; XYLENES; MERCURY; SOILS; REMEDIAL ACTION

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                    Hammes, U. Comparison of immunoassay field tests and laboratory results for PCB, PAH, BTEX, and mercury contaminated soils.  United States: N. p., 1995. 
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                    Hammes, U. Comparison of immunoassay field tests and laboratory results for PCB, PAH, BTEX, and mercury contaminated soils.  United States.

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                    Hammes, U. Fri .  
        "Comparison of immunoassay field tests and laboratory results for PCB, PAH, BTEX, and mercury contaminated soils".  United States.

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@article{osti_122445,

  title        = {Comparison of immunoassay field tests and laboratory results for PCB, PAH, BTEX, and mercury contaminated soils},

  author       = {Hammes, U},

  abstractNote = {Immunoassay tests were used as in situ field screening tools for simultaneous assessment and remediation of soil contaminated with mercury and organics (polychlorinated biphenyls (PCB), polyaromatic hydrocarbons (PAH), and BTEX). Soil samples from approximately 200 sites including metering and compressor stations were investigated along gas pipelines. The suspected contamination originated from formerly used mercury manometers and pipeline liquids. An enzyme-linked immunosorbent assay (ELISA) was used for the detection of organic and mercury contaminants. ELISA combines selective antibodies with sensitive enzyme reactions to produce semiquantitative analytical systems capable of detecting very low levels of chemicals. Color sample tubes were compared to the color of standard tubes to semiquantitate the amount of mercury, PCB, PAH, and BBTEX present in the samples. Two standards were tested in order to eliminate the effects of false negatives (e.g., contaminant not detected when present) or false positives (e.g., contamination detected when not present). For verification purposes, selected samples that were determined to be below action level with the immunoassay tests were sent to the laboratory.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/122445},
  journal      = {Ground Water},
number       = 5,

  volume       = 33,

  place        = {United States},

  year         = {1995},

  month        = {9}

}

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