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Title: Rapid microfluidic thermal cycler for nucleic acid amplification

Abstract

A system for thermal cycling a material to be thermal cycled including a microfluidic heat exchanger; a porous medium in the microfluidic heat exchanger; a microfluidic thermal cycling chamber containing the material to be thermal cycled, the microfluidic thermal cycling chamber operatively connected to the microfluidic heat exchanger; a working fluid at first temperature; a first system for transmitting the working fluid at first temperature to the microfluidic heat exchanger; a working fluid at a second temperature, a second system for transmitting the working fluid at second temperature to the microfluidic heat exchanger; a pump for flowing the working fluid at the first temperature from the first system to the microfluidic heat exchanger and through the porous medium; and flowing the working fluid at the second temperature from the second system to the heat exchanger and through the porous medium.

Inventors:
;
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1224444
Patent Number(s):
9,170,060
Application Number:
12/270,348
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA) LLNL
DOE Contract Number:  
AC52-07NA27344
Resource Type:
Patent
Resource Relation:
Patent File Date: 2008 Nov 13
Country of Publication:
United States
Language:
English
Subject:
42 ENGINEERING

Citation Formats

Beer, Neil Reginald, and Vafai, Kambiz. Rapid microfluidic thermal cycler for nucleic acid amplification. United States: N. p., 2015. Web.
Beer, Neil Reginald, & Vafai, Kambiz. Rapid microfluidic thermal cycler for nucleic acid amplification. United States.
Beer, Neil Reginald, and Vafai, Kambiz. Tue . "Rapid microfluidic thermal cycler for nucleic acid amplification". United States. doi:. https://www.osti.gov/servlets/purl/1224444.
@article{osti_1224444,
title = {Rapid microfluidic thermal cycler for nucleic acid amplification},
author = {Beer, Neil Reginald and Vafai, Kambiz},
abstractNote = {A system for thermal cycling a material to be thermal cycled including a microfluidic heat exchanger; a porous medium in the microfluidic heat exchanger; a microfluidic thermal cycling chamber containing the material to be thermal cycled, the microfluidic thermal cycling chamber operatively connected to the microfluidic heat exchanger; a working fluid at first temperature; a first system for transmitting the working fluid at first temperature to the microfluidic heat exchanger; a working fluid at a second temperature, a second system for transmitting the working fluid at second temperature to the microfluidic heat exchanger; a pump for flowing the working fluid at the first temperature from the first system to the microfluidic heat exchanger and through the porous medium; and flowing the working fluid at the second temperature from the second system to the heat exchanger and through the porous medium.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Oct 27 00:00:00 EDT 2015},
month = {Tue Oct 27 00:00:00 EDT 2015}
}

Patent:

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Works referenced in this record:

Rapid microfluidic thermal cycler for polymerase chain reaction nucleic acid amplification
journal, May 2008


On-Chip Single-Copy Real-Time Reverse-Transcription PCR in Isolated Picoliter Droplets
journal, March 2008

  • Beer, N. Reginald; Wheeler, Elizabeth K.; Lee-Houghton, Lorenna
  • Analytical Chemistry, Vol. 80, Issue 6, p. 1854-1858
  • DOI: 10.1021/ac800048k