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Title: Structural and functional characterization of the hazelnut allergen Cor a 8

Journal Article · · Journal of Agricultural and Food Chemistry
 [1];  [2];  [3];  [2];  [2];  [4];  [3];  [2]
  1. Univ. of South Carolina, Columbia, SC (United States); Davidson College, Davidson, NC (United States)
  2. Univ. of Vienna, Vienna (Austria)
  3. Univ. of South Carolina, Columbia, SC (United States)
  4. Polish Academy of Sciences, Krakow (Poland)
+ Show Author Affiliations

Nonspecific lipid transfer proteins (nsLTPs) are basic proteins, stabilized by four disulfide bonds, and are expressed throughout the plant kingdom. These proteins are also known as important allergens in fruits and tree nuts. In this study, the nsLTP from hazelnuts, Cor a 8, was purified and its crystal structure determined. The protein is stable at low pH and refolds after thermal denaturation. Molecular dynamics simulations were used to provide an insight into conformational changes of Cor a 8 upon ligand binding. When known epitope areas from Pru p 3 were compared to those of Cor a 8, differences were obvious, which may contribute to limited cross-reactivity between peach and hazelnut allergens. The differences in epitope regions may contribute to limited cross-reactivity between Cor a 8 and nsLTPs from other plant sources. The structure of Cor a 8 represents the first resolved structure of a hazelnut allergen.

Research Organization:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
AC02-06CH11357; W-31-109-ENG-38; SFB F4603; W1248
OSTI ID:
1224206
Journal Information:
Journal of Agricultural and Food Chemistry, Vol. 63, Issue 41; ISSN 0021-8561
Publisher:
American Chemical SocietyCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 29 works
Citation information provided by
Web of Science

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Cited By (6)

Lipid transfer proteins: classification, nomenclature, structure, and function journal August 2016
Protective role of hazelnut peptides on oxidative stress injury in human umbilical vein endothelial cells journal December 2018
Elimination of the major allergen tropomyosin from shrimp muscle by boiling treatment journal November 2019
Impact of lipid binding on the tertiary structure and allergenic potential of Jug r 3, the non-specific lipid transfer protein from walnut journal February 2019
IgE and IgG4 binding to lentil epitopes in children with red and green lentil allergy journal November 2019
A robust method for the estimation and visualization of IgE cross-reactivity likelihood between allergens belonging to the same protein family journal November 2018

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
allergen
food allergy
hazelnut
nonspecific lipid transfer protein
protein crystallization