Over-expression of NADH-dependent oxidoreductase (fucO) for increasing furfural or 5-hydroxymethylfurfural tolerance

Miller, Elliot N.; Zhang, Xueli; Yomano, Lorraine P.; Wang, Xuan; Shanmugam, Keelnatham T.; Ingram, Lonnie O'Neal

Over-expression of NADH-dependent oxidoreductase (fucO) for increasing furfural or 5-hydroxymethylfurfural tolerance

Patent · 13 October 2015

OSTI ID:1223111

Miller, Elliot N.; Zhang, Xueli; Yomano, Lorraine P.; Wang, Xuan; Shanmugam, Keelnatham T.; Ingram, Lonnie O'Neal

The subject invention pertains to the discovery that the NADH-dependent propanediol oxidoreductase (FucO) can reduce furfural. This allows for a new approach to improve furfural tolerance in bacterial and/or yeast cells used to produce desired products. Thus, novel biocatalysts (bacterial, fungal or yeast cells) exhibiting increased tolerance to furfural and 5-hydroxymethylfurfural (5-HMF) are provided as are methods of making and using such biocatalysts for the production of a desired product.

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Research Organization:: University of Florida Research Foundation, Inc., Gainesville, FL (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG36-08GO88142

Assignee:: University of Florida Research Foundation, Inc. (Gainesville, FL)

Patent Number(s):: 9,157,102

Application Number:: 14/008,015

OSTI ID:: 1223111

Country of Publication:: United States

Language:: English

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