skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Over-expression of NADH-dependent oxidoreductase (fucO) for increasing furfural or 5-hydroxymethylfurfural tolerance

Abstract

The subject invention pertains to the discovery that the NADH-dependent propanediol oxidoreductase (FucO) can reduce furfural. This allows for a new approach to improve furfural tolerance in bacterial and/or yeast cells used to produce desired products. Thus, novel biocatalysts (bacterial, fungal or yeast cells) exhibiting increased tolerance to furfural and 5-hydroxymethylfurfural (5-HMF) are provided as are methods of making and using such biocatalysts for the production of a desired product.

Inventors:
; ; ; ; ;
Publication Date:
Research Org.:
University of Florida Research Foundation, Inc., Gainesville, FL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1223111
Patent Number(s):
9,157,102
Application Number:
14/008,015
Assignee:
University of Florida Research Foundation, Inc. (Gainesville, FL) GFO
DOE Contract Number:  
FG36-08GO88142
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 Mar 29
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Miller, Elliot N., Zhang, Xueli, Yomano, Lorraine P., Wang, Xuan, Shanmugam, Keelnatham T., and Ingram, Lonnie O'Neal. Over-expression of NADH-dependent oxidoreductase (fucO) for increasing furfural or 5-hydroxymethylfurfural tolerance. United States: N. p., 2015. Web.
Miller, Elliot N., Zhang, Xueli, Yomano, Lorraine P., Wang, Xuan, Shanmugam, Keelnatham T., & Ingram, Lonnie O'Neal. Over-expression of NADH-dependent oxidoreductase (fucO) for increasing furfural or 5-hydroxymethylfurfural tolerance. United States.
Miller, Elliot N., Zhang, Xueli, Yomano, Lorraine P., Wang, Xuan, Shanmugam, Keelnatham T., and Ingram, Lonnie O'Neal. Tue . "Over-expression of NADH-dependent oxidoreductase (fucO) for increasing furfural or 5-hydroxymethylfurfural tolerance". United States. https://www.osti.gov/servlets/purl/1223111.
@article{osti_1223111,
title = {Over-expression of NADH-dependent oxidoreductase (fucO) for increasing furfural or 5-hydroxymethylfurfural tolerance},
author = {Miller, Elliot N. and Zhang, Xueli and Yomano, Lorraine P. and Wang, Xuan and Shanmugam, Keelnatham T. and Ingram, Lonnie O'Neal},
abstractNote = {The subject invention pertains to the discovery that the NADH-dependent propanediol oxidoreductase (FucO) can reduce furfural. This allows for a new approach to improve furfural tolerance in bacterial and/or yeast cells used to produce desired products. Thus, novel biocatalysts (bacterial, fungal or yeast cells) exhibiting increased tolerance to furfural and 5-hydroxymethylfurfural (5-HMF) are provided as are methods of making and using such biocatalysts for the production of a desired product.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Oct 13 00:00:00 EDT 2015},
month = {Tue Oct 13 00:00:00 EDT 2015}
}

Patent:

Save / Share:

Works referenced in this record:

Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: A review
journal, July 2010


Optimizing the saccharification of sugar cane bagasse using dilute phosphoric acid followed by fungal cellulases
journal, March 2010


Simplified process for ethanol production from sugarcane bagasse using hydrolysate-resistant Escherichia coli strain MM160
journal, February 2011


Combining metabolic engineering and metabolic evolution to develop nonrecombinant strains of Escherichia coli C that produce succinate and malate
journal, January 2008

  • Jantama, Kaemwich; Haupt, M. J.; Svoronos, Spyros A.
  • Biotechnology and Bioengineering, Vol. 99, Issue 5, p. 1140-1153
  • DOI: 10.1002/bit.21694

Effect of alcohol compounds found in hemicellulose hydrolysate on the growth and fermentation of ethanologenic Escherichia coli
journal, June 2000


Effect of selected aldehydes on the growth and fermentation of ethanologenic Escherichia coli
journal, October 1999


Metabolic evolution of energy-conserving pathways for succinate production in Escherichia coli
journal, November 2009

  • Zhang, X.; Jantama, K.; Moore, J. C.
  • Proceedings of the National Academy of Sciences, Vol. 106, Issue 48, p. 20180-20185
  • DOI: 10.1073/pnas.0905396106