Solid-state NMR studies of proteins immobilized on inorganic surfaces

Shaw, Wendy J.

doi:10.1016/j.ssnmr.2014.10.003

Title: Solid-state NMR studies of proteins immobilized on inorganic surfaces

Journal Article · Wed Oct 29 00:00:00 EDT 2014 · Solid State Nuclear Magnetic Resonance

DOI:https://doi.org/10.1016/j.ssnmr.2014.10.003· OSTI ID:1222894

Shaw, Wendy J. ^[1]

Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Solid state NMR is the primary tool for studying the quantitative, site-specific structure, orientation, and dynamics of biomineralization proteins under biologically relevant conditions. Two calcium phosphate proteins, statherin and leucine rich amelogenin protein (LRAP), have been studied in depth and have different features, challenging our ability to extract design principles. More recent studies of the significantly larger full-length amelogenin represent a challenging but necessary step to ultimately investigate the full diversity of biomineralization proteins. Interactions of amino acids and silaffin peptide with silica are also being studied, along with qualitative studies of proteins interacting with calcium carbonate. Dipolar recoupling techniques have formed the core of the quantitative studies, yet, the need for isolated spin pairs makes this approach costly and time intensive. The use of multi-dimensional techniques is advancing, methodology which, despite its challenges with these difficult-to-study proteins, will continue to drive future advancements in this area.

View Accepted Manuscript (DOE)

View Accepted Manuscript (Publisher)

Cite

Export

Save

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-76RL01830; DE-015347

OSTI ID:: 1222894

Alternate ID(s):: OSTI ID: 1250248

Report Number(s):: PNNL-SA-104618; 400412000

Journal Information:: Solid State Nuclear Magnetic Resonance, Vol. 70, Issue C; ISSN 0926-2040

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 20 works

Citation information provided by
Web of Science

References (71)

Self-Assembly Properties of Recombinant Engineered Amelogenin Proteins Analyzed by Dynamic Light Scattering and Atomic Force Microscopy Moradian-Oldak, J.; Paine, M. L.; Lei, Y. P. Journal of Structural Biology, Vol. 131, Issue 1 https://doi.org/10.1006/jsbi.2000.4237	journal	July 2000
Altered Amelogenin Self-assembly Based on Mutations Observed in Human X-linked Amelogenesis Imperfecta (AIH1) Paine, Michael L.; Lei, Ya-Ping; Dickerson, Kenneth Journal of Biological Chemistry, Vol. 277, Issue 19 https://doi.org/10.1074/jbc.M110473200	journal	May 2002
Intrinsic Disorder and Protein Function Dunker, A. Keith; Brown, Celeste J.; Lawson, J. David Biochemistry, Vol. 41, Issue 21 https://doi.org/10.1021/bi012159+	journal	May 2002
Proton‐enhanced NMR of dilute spins in solids Pines, A.; Gibby, M. G.; Waugh, J. S. The Journal of Chemical Physics, Vol. 59, Issue 2 https://doi.org/10.1063/1.1680061	journal	July 1973
Windowless dipolar recoupling: the detection of weak dipolar couplings between spin 12 nuclei with large chemical shift anisotropies Gregory, D. M.; Mitchell, D. J.; Stringer, J. A. Chemical Physics Letters, Vol. 246, Issue 6 https://doi.org/10.1016/0009-2614(95)01166-3	journal	December 1995
9-Fluorenylmethoxycarbonyl amino-protecting group Carpino, Louis A.; Han, Grace Y. The Journal of Organic Chemistry, Vol. 37, Issue 22 https://doi.org/10.1021/jo00795a005	journal	November 1972
S TRUCTURAL S TUDIES OF B IOMATERIALS U SING D OUBLE -Q UANTUM S OLID -S TATE NMR S PECTROSCOPY Drobny, G. P.; Long, J. R.; Karlsson, T. Annual Review of Physical Chemistry, Vol. 54, Issue 1 https://doi.org/10.1146/annurev.physchem.54.011002.103903	journal	October 2003
Solid-State NMR Studies of Biomineralization Peptides and Proteins Roehrich, Adrienne; Drobny, Gary Accounts of Chemical Research, Vol. 46, Issue 9 https://doi.org/10.1021/ar300321e	journal	June 2013
Hydrogen Bond Formation between Citrate and Phosphate Ions in Spherulites of Fluorapatite Wu, Yu-Ju; Tsai, Tim W. T.; Huang, Shing-Jong Langmuir, Vol. 29, Issue 37 https://doi.org/10.1021/la402392b	journal	September 2013
Solid-state P-31 NMR study of octacalcium phosphate incorporated with succinate Tsai, Tim W. T.; Chou, Fang-Chieh; Tseng, Yao-Hung Physical Chemistry Chemical Physics, Vol. 12, Issue 25 https://doi.org/10.1039/b923338e	journal	January 2010
Conformation and Dynamics of Organic Tethers Bound to MCM41-Type Surfaces from Solid State NMR Measurements Ghindes-Azaria, Lee; Levy, Esthy; Keinan-Adamsky, Keren The Journal of Physical Chemistry C, Vol. 116, Issue 13 https://doi.org/10.1021/jp212524f	journal	March 2012
Characterization of the Crystallization Pathway of Calcium Phosphate in Liposomes Chen, Wei-Ya; Yang, Chien-I; Lin, Cang-Jie The Journal of Physical Chemistry C, Vol. 118, Issue 22 https://doi.org/10.1021/jp502428k	journal	May 2014
Past and Future Solid-State NMR Spectroscopy Studies at the Convergence Point between Biology and Materials Research Goobes, Gil Israel Journal of Chemistry, Vol. 54, Issue 1-2 https://doi.org/10.1002/ijch.201300113	journal	February 2014
Phase transformation of calcium phosphates in the presence of glutamic acid Tsai, Tim W. T.; Chen, Wei-Ya; Tseng, Yao-Hung Canadian Journal of Chemistry, Vol. 89, Issue 7 https://doi.org/10.1139/v11-032	journal	July 2011
Solid-State NMR Study of the Transformation of Octacalcium Phosphate to Hydroxyapatite: A Mechanistic Model for Central Dark Line Formation Tseng, Yao-Hung; Mou, Chung-Yuan; Chan, Jerry C. C. Journal of the American Chemical Society, Vol. 128, Issue 21 https://doi.org/10.1021/ja060336u	journal	May 2006
Solid-state P-31 NMR study of the formation of hydroxyapatite in the presence of glutaric acid Tseng, Yao-Hung; Mou, Yun; Chen, Peng-Huan Magnetic Resonance in Chemistry, Vol. 46, Issue 4 https://doi.org/10.1002/mrc.2096	journal	January 2008
Characterization of the Phosphate Units in Rat Dentin by Solid-State NMR Spectroscopy Tseng, Yao-Hung; Tsai, Yi-Ling; Tsai, Tim W. T. Chemistry of Materials, Vol. 19, Issue 25 https://doi.org/10.1021/cm070531n	journal	November 2007
High resolution 31P NMR study of octacalcium phosphate Tseng, Yao-Hung; Zhan, Jinhua; Lin, Kyle S. K. Solid State Nuclear Magnetic Resonance, Vol. 26, Issue 2 https://doi.org/10.1016/j.ssnmr.2004.06.002	journal	September 2004
Recent Progress in the Solid-State NMR Studies of Biomineralization Tsai, Tim W. T.; Chan, Jerry C. C. Annual Reports on NMR Spectroscopy https://doi.org/10.1016/B978-0-08-097074-5.00001-3	book	January 2011
Magnetic resonance studies of polypeptides adsorbed on silica and hydroxyapatite surfaces Fernandez, Veronica L.; Reimer, Jeffrey A.; Denn, Morton M. Journal of the American Chemical Society, Vol. 114, Issue 24 https://doi.org/10.1021/ja00050a051	journal	November 1992
Complete covalent structure of statherin, a tyrosine-rich acidic peptide which inhibits calcium phosphate precipitation from human parotid saliva. Schlesinger, D. H.; Hay, D. I. Journal of Biological Chemistry, Vol. 252, Issue 5 https://doi.org/10.1016/S0021-9258(17)40603-X	journal	March 1977
Salivary statherin. Dependence on sequence, charge, hydrogen bonding potency, and helical conformation for adsorption to hydroxyapatite and inhibition of mineralization. Raj, P. A.; Johnsson, M.; Levine, M. J. Journal of Biological Chemistry, Vol. 267, Issue 9 https://doi.org/10.1016/S0021-9258(18)42650-6	journal	March 1992
M olecular R ecognition at the P rotein- H ydroxyapatite I nterface Stayton, Patrick S.; Drobny, Gary P.; Shaw, Wendy J. Critical Reviews in Oral Biology & Medicine, Vol. 14, Issue 5 https://doi.org/10.1177/154411130301400507	journal	September 2003
The structure, dynamics, and energetics of protein adsorption—lessons learned from adsorption of statherin to hydroxyapatite Goobes, Gil; Goobes, Rivka; Shaw, Wendy J. Magnetic Resonance in Chemistry, Vol. 45, Issue S1 https://doi.org/10.1002/mrc.2123	journal	December 2007
Solid state NMR studies of molecular recognition at protein–mineral interfaces Goobes, Gil; Stayton, Patrick S.; Drobny, Gary P. Progress in Nuclear Magnetic Resonance Spectroscopy, Vol. 50, Issue 2-3 https://doi.org/10.1016/j.pnmrs.2006.11.002	journal	March 2007
Toward a Structure Determination Method for Biomineral-Associated Protein Using Combined Solid- State NMR and Computational Structure Prediction Masica, David L.; Ash, Jason T.; Ndao, Moise Structure, Vol. 18, Issue 12 https://doi.org/10.1016/j.str.2010.09.013	journal	December 2010
Structure and Dynamics of Hydrated Statherin on Hydroxyapatite As Determined by Solid-State NMR Long, Joanna R.; Shaw, Wendy J.; Stayton, Patrick S. Biochemistry, Vol. 40, Issue 51 https://doi.org/10.1021/bi010864c	journal	November 2001
A Solid State NMR Study of Dynamics in a Hydrated Salivary Peptide Adsorbed to Hydroxyapatite Shaw, Wendy J.; Long, Joanna R.; Campbell, Allison A. Journal of the American Chemical Society, Vol. 122, Issue 29 https://doi.org/10.1021/ja000878q	journal	July 2000
Determination of Statherin N-Terminal Peptide Conformation on Hydroxyapatite Crystals Shaw, Wendy J.; Long, Joanna R.; Dindot, John L. Journal of the American Chemical Society, Vol. 122, Issue 8 https://doi.org/10.1021/ja9842823	journal	March 2000
Folding of the C-terminal bacterial binding domain in statherin upon adsorption onto hydroxyapatite crystals Goobes, G.; Goobes, R.; Schueler-Furman, O. Proceedings of the National Academy of Sciences, Vol. 103, Issue 44 https://doi.org/10.1073/pnas.0607193103	journal	October 2006
A Solid-State NMR Study of the Dynamics and Interactions of Phenylalanine Rings in a Statherin Fragment Bound to Hydroxyapatite Crystals Gibson, James M.; Popham, Jennifer M.; Raghunathan, Vinodhkumar Journal of the American Chemical Society, Vol. 128, Issue 16 https://doi.org/10.1021/ja056731m	journal	April 2006
Direct Observation of Phenylalanine Orientations in Statherin Bound to Hydroxyapatite Surfaces Weidner, Tobias; Dubey, Manish; Breen, Nicholas F. Journal of the American Chemical Society, Vol. 134, Issue 21 https://doi.org/10.1021/ja301711w	journal	May 2012
A REDOR NMR Study of a Phosphorylated Statherin Fragment Bound to Hydroxyapatite Crystals Gibson, James M.; Raghunathan, Vinodhkumar; Popham, Jennifer M. Journal of the American Chemical Society, Vol. 127, Issue 26 https://doi.org/10.1021/ja050910m	journal	July 2005
The role of basic amino acids in the molecular recognition of hydroxyapatite by statherin using solid state NMR Ndao, Moise; Ash, Jason T.; Stayton, Patrick S. Surface Science, Vol. 604, Issue 15-16 https://doi.org/10.1016/j.susc.2010.02.026	journal	August 2010
A ¹³ C{ ³¹ P} REDOR NMR Investigation of the Role of Glutamic Acid Residues in Statherin- Hydroxyapatite Recognition Ndao, Moise; Ash, Jason T.; Breen, Nicholas F. Langmuir, Vol. 25, Issue 20 https://doi.org/10.1021/la901647n	journal	August 2009
Interfacial aspects of biomineralization Hunter, Graeme K. Current Opinion in Solid State and Materials Science, Vol. 1, Issue 3 https://doi.org/10.1016/S1359-0286(96)80036-2	journal	June 1996
The Flexible Polyelectrolyte Hypothesis of Protein−Biomineral Interaction Hunter, Graeme K.; O’Young, Jason; Grohe, Bernd Langmuir, Vol. 26, Issue 24 https://doi.org/10.1021/la100401r	journal	December 2010
Solution- and Adsorbed-State Structural Ensembles Predicted for the Statherin-Hydroxyapatite System Masica, David L.; Gray, Jeffrey J. Biophysical Journal, Vol. 96, Issue 8 https://doi.org/10.1016/j.bpj.2009.01.033	journal	April 2009
Controlled proteolysis of amelogenins reveals exposure of both carboxy- and amino-terminal regions Moradian-Oldak, J.; Jimenez, I.; Maltby, D. Biopolymers, Vol. 58, Issue 7 https://doi.org/10.1002/1097-0282(200106)58:7<606::AID-BIP1034>3.0.CO;2-8	journal	January 2001
Interaction of amelogenin with hydroxyapatite crystals: An adherence effect through amelogenin molecular self-association Moradian-Oldak, J.; Tan, J.; Fincham, A. G. Biopolymers, Vol. 46, Issue 4 https://doi.org/10.1002/(SICI)1097-0282(19981005)46:4<225::AID-BIP4>3.0.CO;2-R	journal	October 1998
A Solution NMR Investigation into the Early Events of Amelogenin Nanosphere Self-Assembly Initiated with Sodium Chloride or Calcium Chloride Buchko, Garry W.; Tarasevich, Barbara J.; Bekhazi, Jacky Biochemistry, Vol. 47, Issue 50 https://doi.org/10.1021/bi8018288	journal	November 2008
Structure, Orientation, and Dynamics of the C-Terminal Hexapeptide of LRAP Determined Using Solid-State NMR Shaw, Wendy J.; Ferris, Kim The Journal of Physical Chemistry B, Vol. 112, Issue 51 https://doi.org/10.1021/jp808012g	journal	December 2008
The Structure and Orientation of the C-Terminus of LRAP Shaw, Wendy J.; Ferris, Kim; Tarasevich, Barbara Biophysical Journal, Vol. 94, Issue 8 https://doi.org/10.1529/biophysj.107.119636	journal	April 2008
The COOH Terminus of the Amelogenin, LRAP, Is Oriented Next to the Hydroxyapatite Surface Shaw, Wendy J.; Campbell, Allison A.; Paine, Michael L. Journal of Biological Chemistry, Vol. 279, Issue 39 https://doi.org/10.1074/jbc.C400322200	journal	August 2004
The effects of human salivary cystatins and statherin on hydroxyapatite crystallization Johnsson, M.; Richardson, C. F.; Bergey, E. J. Archives of Oral Biology, Vol. 36, Issue 9 https://doi.org/10.1016/0003-9969(91)90014-L	journal	January 1991
Analysis of Hydroxyapatite Surface Coverage by Amelogenin Nanospheres Following the Langmuir Model for Protein Adsorption Bouropoulos, N.; Moradian–Oldak, J. Calcified Tissue International, Vol. 72, Issue 5 https://doi.org/10.1007/s00223-002-1099-1	journal	April 2003
Possible Roles of Partial Sequences at N- and C-termini of Amelogenin in Protein-Enamel Mineral Interaction Aoba, T.; Moreno, E. C.; Kresak, M. Journal of Dental Research, Vol. 68, Issue 9 https://doi.org/10.1177/00220345890680090901	journal	September 1989
Protein Structure Determination by Magic-Angle Spinning Solid-State NMR, and Insights into the Formation, Structure, and Stability of Amyloid Fibrils Comellas, Gemma; Rienstra, Chad M. Annual Review of Biophysics, Vol. 42, Issue 1 https://doi.org/10.1146/annurev-biophys-083012-130356	journal	May 2013
Advanced Solid-State NMR Approaches for Structure Determination of Membrane Proteins and Amyloid Fibrils Tang, Ming; Comellas, Gemma; Rienstra, Chad M. Accounts of Chemical Research, Vol. 46, Issue 9 https://doi.org/10.1021/ar4000168	journal	May 2013
Empirical correlation between protein backbone conformation and C.alpha. and C.beta. 13C nuclear magnetic resonance chemical shifts Spera, Silvia; Bax, Ad Journal of the American Chemical Society, Vol. 113, Issue 14 https://doi.org/10.1021/ja00014a071	journal	July 1991
Mineral Association Changes the Secondary Structure and Dynamics of Murine Amelogenin Lu, J. X.; Xu, Y. S.; Buchko, G. W. Journal of Dental Research, Vol. 92, Issue 11 https://doi.org/10.1177/0022034513504929	journal	October 2013
Adsorption of a Statherin Peptide Fragment on the Surface of Nanocrystallites of Hydroxyapatite Chen, Peng-Huan; Tseng, Yao-Hung; Mou, Yun Journal of the American Chemical Society, Vol. 130, Issue 9 https://doi.org/10.1021/ja076607y	journal	February 2008
Intrinsically unstructured proteins Tompa, Peter Trends in Biochemical Sciences, Vol. 27, Issue 10 https://doi.org/10.1016/S0968-0004(02)02169-2	journal	October 2002
Protein- and Peptide-Directed Syntheses of Inorganic Materials Dickerson, Matthew B.; Sandhage, Kenneth H.; Naik, Rajesh R. Chemical Reviews, Vol. 108, Issue 11, p. 4935-4978 https://doi.org/10.1021/cr8002328	journal	November 2008
A Dynamic Magic Angle Spinning NMR Study of the Local Mobility of Alanine in an Aqueous Environment at the Inner Surface of Mesoporous Materials Amitay-Rosen, Tal; Kababya, Shifi; Vega, Shimon The Journal of Physical Chemistry B, Vol. 113, Issue 18 https://doi.org/10.1021/jp810572r	journal	April 2009
A deuterium MAS NMR study of the local mobility of dissolved methionine and di-alanine at the inner surface of SBA-15 Amitay-Rosen, Tal; Vega, Shimon Physical Chemistry Chemical Physics, Vol. 12, Issue 25 https://doi.org/10.1039/b924813g	journal	January 2010
NMR Study of the Adsorption−Desorption Kinetics of Dissolved Tetraalanine in MCM-41 Mesoporous Material Pizzanelli, Silvia; Kababya, Shifi; Frydman, Veronica The Journal of Physical Chemistry B, Vol. 109, Issue 16 https://doi.org/10.1021/jp044389e	journal	April 2005
Molecular Level Characterization of the Inorganic−Bioorganic Interface by Solid State NMR: Alanine on a Silica Surface, a Case Study Ben Shir, Ira; Kababya, Shifi; Amitay-Rosen, Tal The Journal of Physical Chemistry B, Vol. 114, Issue 18 https://doi.org/10.1021/jp100114v	journal	April 2010
Binding Specificity of Amino Acids to Amorphous Silica Surfaces: Solid-State NMR of Glycine on SBA-15 Ben Shir, Ira; Kababya, Shifi; Schmidt, Asher The Journal of Physical Chemistry C, Vol. 116, Issue 17 https://doi.org/10.1021/jp302431t	journal	April 2012
Molecular Details of Amorphous Silica Surfaces Determine Binding Specificity to Small Amino Acids Ben Shir, Ira; Kababya, Shifi; Schmidt, Asher The Journal of Physical Chemistry C, Vol. 118, Issue 15 https://doi.org/10.1021/jp409030w	journal	April 2014
In situ molecular NMR picture of bioavailable calcium stabilized as amorphous CaCO3 biomineral in crayfish gastroliths Akiva-Tal, A.; Kababya, S.; Balazs, Y. S. Proceedings of the National Academy of Sciences, Vol. 108, Issue 36 https://doi.org/10.1073/pnas.1102608108	journal	August 2011
Fine structure of nacre revealed by solid state 13C and 1H NMR Jäger, Christian; Cölfen, Helmut CrystEngComm, Vol. 9, Issue 12 https://doi.org/10.1039/b708600h	journal	January 2007
In Situ Observation of the Internal Structure and Composition of Biomineralized Emiliania huxleyi Calcite by Solid-State NMR Spectroscopy Gertman, Ronen; Ben Shir, Ira; Kababya, Shifi Journal of the American Chemical Society, Vol. 130, Issue 40 https://doi.org/10.1021/ja803985d	journal	October 2008
Investigating hyperpolarized 129Xe and CPMAS for spin polarization transfer to surface nuclei: a model study Smith, Jay; Knagge, Kevin; Smith, Luis J. Journal of Magnetic Resonance, Vol. 159, Issue 2 https://doi.org/10.1016/S1090-7807(02)00015-0	journal	December 2002
High Frequency Dynamic Nuclear Polarization Ni, Qing Zhe; Daviso, Eugenio; Can, Thach V. Accounts of Chemical Research, Vol. 46, Issue 9 https://doi.org/10.1021/ar300348n	journal	April 2013
Molecular simulation of biomineral nucleation and crystal growth: Modern computational challenges and approaches Yang, Yang; Xu, Zhijun; Cui, Qiang Biomineralization Sourcebook https://doi.org/10.1201/b16621-17	book	February 2014
Subnanometer atomic force microscopy of peptide-mineral interactions links clustering and competition to acceleration and catastrophe Friddle, R. W.; Weaver, M. L.; Qiu, S. R. Proceedings of the National Academy of Sciences, Vol. 107, Issue 1 https://doi.org/10.1073/pnas.0908205107	journal	December 2009
Regulation of in vitro Calcium Phosphate Mineralization by Combinatorially Selected Hydroxyapatite-Binding Peptides Gungormus, Mustafa; Fong, Hanson; Kim, Il Won Biomacromolecules, Vol. 9, Issue 3 https://doi.org/10.1021/bm701037x	journal	March 2008
Cementomimetics—constructing a cementum-like biomineralized microlayer via amelogenin-derived peptides Gungormus, Mustafa; Oren, Ersin E.; Horst, Jeremy A. International Journal of Oral Science, Vol. 4, Issue 2 https://doi.org/10.1038/ijos.2012.40	journal	June 2012
Molecular-Level StructureProperty Relationships in Biogenic Calcium Carbonates: The Unique Insights of Solid-State NMR Spectroscopy Shir, Ira Ben; Kababya, Shifi; Schmidt, Asher Israel Journal of Chemistry, Vol. 54, Issue 1-2 https://doi.org/10.1002/ijch.201300121	journal	January 2014
Thermodynamic Roles of Basic Amino Acids in Statherin Recognition of Hydroxyapatite Goobes, Rivka; Goobes, Gil; Shaw, Wendy J. Biochemistry, Vol. 46, Issue 16 https://doi.org/10.1021/bi602345a	journal	March 2007

Cited By (6)

Controlling protein assembly on inorganic crystals through designed protein interfaces Pyles, Harley; Zhang, Shuai; De Yoreo, James J. Nature, Vol. 571, Issue 7764 https://doi.org/10.1038/s41586-019-1361-6	journal	July 2019
Bone mineral: new insights into its chemical composition Von Euw, Stanislas; Wang, Yan; Laurent, Guillaume Scientific Reports, Vol. 9, Issue 1 https://doi.org/10.1038/s41598-019-44620-6	journal	June 2019
Molecular insights into hypomineralized enamel Malmberg, Per; Norén, Jörgen G.; Bernin, Diana European Journal of Oral Sciences, Vol. 127, Issue 4 https://doi.org/10.1111/eos.12619	journal	April 2019
Atomic-Level Quality Assessment of Enzymes Encapsulated in Bioinspired Silica Martelli, Tommaso; Ravera, Enrico; Louka, Alexandra Chemistry - A European Journal, Vol. 22, Issue 1 https://doi.org/10.1002/chem.201503613	journal	December 2015
PyRosetta: a script-based interface for implementing molecular modeling algorithms using Rosetta Chaudhury, S.; Lyskov, S.; Gray, J. J. Bioinformatics, Vol. 26, Issue 5, p. 689-691 https://doi.org/10.1093/bioinformatics/btq007	journal	January 2010
Nano-Mole Scale Side-Chain Signal Assignment by 1H-Detected Protein Solid-State NMR by Ultra-Fast Magic-Angle Spinning and Stereo-Array Isotope Labeling Wang, Songlin; Parthasarathy, Sudhakar; Nishiyama, Yusuke PLOS ONE, Vol. 10, Issue 4 https://doi.org/10.1371/journal.pone.0122714	journal	April 2015

Similar Records

The leucine-rich amelogenin protein (LRAP) is primarily monomeric and unstructured in physiological solution

Journal Article · Sat Oct 25 00:00:00 EDT 2014 · Journal of Structural Biology · OSTI ID:1222894

Tarasevich, Barbara J.; Philo, John S.; Maluf, Nasib Karl; +4 more

Structure and Dynamics of Hydrated Statherin on Hydroxyapatite As Determined by Solid-State NMR

Journal Article · Tue Dec 25 00:00:00 EST 2001 · Biochemistry, 40(51):15451-15455 · OSTI ID:1222894

Long, Joanna; Shaw, Wendy J; Stayton, Patrick; +1 more

Neutron Reflectometry Studies of the Adsorbed Structure of the Amelogenin, LRAP

Journal Article · Thu Mar 21 00:00:00 EDT 2013 · Journal of Physical Chemistry B, 117(11):3098-3109 · OSTI ID:1222894

Tarasevich, Barbara J.; Perez-Salas, Ursula; Masica, David L.; +5 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Biomineralization
Immobilized proteins
Dipolar recoupling
Protein structure
Protein dynamics
Protein orientation
Multi-dimensional solid state NMR
Amelogenin
Statherin
Silaffin

Title: Solid-state NMR studies of proteins immobilized on inorganic surfaces

Citation Formats

References (71)

Cited By (6)

Similar Records

Related Subjects