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Title: Indexing Permafrost Soil Organic Matter Degradation Using High-Resolution Mass Spectrometry

Abstract

Microbial degradation of soil organic matter (SOM) is a key process for terrestrial carbon cycling, although the molecular details of these transformations remain unclear. This study reports the application of ultrahigh resolution mass spectrometry to profile the molecular composition of SOM and its degradation during a simulated warming experiment. A soil sample, collected near Barrow, Alaska, USA, was subjected to a 40-day incubation under anoxic conditions and analyzed before and after the incubation to determine changes of SOM composition. A CHO index based on molecular C, H, and O data was utilized to codify SOM components according to their observed degradation potentials. Compounds with a CHO index score between –1 and 0 in a water-soluble fraction (WSF) demonstrated high degradation potential, with a highest shift of CHO index occurred in the N-containing group of compounds, while similar stoichiometries in a base-soluble fraction (BSF) did not. Additionally, compared with the classical H:C vs O:C van Krevelen diagram, CHO index allowed for direct visualization of the distribution of heteroatoms such as N in the identified SOM compounds. We demonstrate that CHO index is useful not only in characterizing arctic SOM at the molecular level but also enabling quantitative description of SOM degradation,more » thereby facilitating incorporation of the high resolution MS datasets to future mechanistic models of SOM degradation and prediction of greenhouse gas emissions.« less

Authors:
; ; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1222888
Report Number(s):
PNNL-SA-111173
48386; KP1704020
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
PLoS One, 10(6):Article No. e0130557
Additional Journal Information:
Journal Name: PLoS One, 10(6):Article No. e0130557
Country of Publication:
United States
Language:
English
Subject:
Environmental Molecular Sciences Laboratory

Citation Formats

Mann, Benjamin F., Chen, Hong-Mei, Herndon, Elizabeth M., Chu, Rosalie K., Tolic, Nikola, Portier, Evan, Chowdhury, Taniya R., Robinson, Errol W., Callister, Stephen J., Wullschleger, Stan D., Graham, David E., Liang, Liyuan, and Gu, Baohua. Indexing Permafrost Soil Organic Matter Degradation Using High-Resolution Mass Spectrometry. United States: N. p., 2015. Web. doi:10.1371/journal.pone.0130557.
Mann, Benjamin F., Chen, Hong-Mei, Herndon, Elizabeth M., Chu, Rosalie K., Tolic, Nikola, Portier, Evan, Chowdhury, Taniya R., Robinson, Errol W., Callister, Stephen J., Wullschleger, Stan D., Graham, David E., Liang, Liyuan, & Gu, Baohua. Indexing Permafrost Soil Organic Matter Degradation Using High-Resolution Mass Spectrometry. United States. doi:10.1371/journal.pone.0130557.
Mann, Benjamin F., Chen, Hong-Mei, Herndon, Elizabeth M., Chu, Rosalie K., Tolic, Nikola, Portier, Evan, Chowdhury, Taniya R., Robinson, Errol W., Callister, Stephen J., Wullschleger, Stan D., Graham, David E., Liang, Liyuan, and Gu, Baohua. Fri . "Indexing Permafrost Soil Organic Matter Degradation Using High-Resolution Mass Spectrometry". United States. doi:10.1371/journal.pone.0130557.
@article{osti_1222888,
title = {Indexing Permafrost Soil Organic Matter Degradation Using High-Resolution Mass Spectrometry},
author = {Mann, Benjamin F. and Chen, Hong-Mei and Herndon, Elizabeth M. and Chu, Rosalie K. and Tolic, Nikola and Portier, Evan and Chowdhury, Taniya R. and Robinson, Errol W. and Callister, Stephen J. and Wullschleger, Stan D. and Graham, David E. and Liang, Liyuan and Gu, Baohua},
abstractNote = {Microbial degradation of soil organic matter (SOM) is a key process for terrestrial carbon cycling, although the molecular details of these transformations remain unclear. This study reports the application of ultrahigh resolution mass spectrometry to profile the molecular composition of SOM and its degradation during a simulated warming experiment. A soil sample, collected near Barrow, Alaska, USA, was subjected to a 40-day incubation under anoxic conditions and analyzed before and after the incubation to determine changes of SOM composition. A CHO index based on molecular C, H, and O data was utilized to codify SOM components according to their observed degradation potentials. Compounds with a CHO index score between –1 and 0 in a water-soluble fraction (WSF) demonstrated high degradation potential, with a highest shift of CHO index occurred in the N-containing group of compounds, while similar stoichiometries in a base-soluble fraction (BSF) did not. Additionally, compared with the classical H:C vs O:C van Krevelen diagram, CHO index allowed for direct visualization of the distribution of heteroatoms such as N in the identified SOM compounds. We demonstrate that CHO index is useful not only in characterizing arctic SOM at the molecular level but also enabling quantitative description of SOM degradation, thereby facilitating incorporation of the high resolution MS datasets to future mechanistic models of SOM degradation and prediction of greenhouse gas emissions.},
doi = {10.1371/journal.pone.0130557},
journal = {PLoS One, 10(6):Article No. e0130557},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {6}
}

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    Works referencing / citing this record:

    Restricted morphospace occupancy of early Cambrian reef-building archaeocyaths
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