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Title: Identification of Natural ROR;#947; Ligands that Regulate the Development of Lymphoid Cells

Authors:
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  1. (TTU)
  2. (
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1222811
Resource Type:
Journal Article
Resource Relation:
Journal Name: Cell Metab.; Journal Volume: 21; Journal Issue: (3) ; 02, 2015
Country of Publication:
United States
Language:
ENGLISH

Citation Formats

Santori, Fabio R., Huang, Pengxiang, van de Pavert, Serge A., Douglass, Jr., Eugene F., Leaver, David J., Haubrich, Brad A., Keber, Rok, Lorbek, Gregor, Konijn, Tanja, Rosales, Brittany N., Rozman, Damjana, Horvat, Simon, Rahier, Alain, Mebius, Reina E., Rastinejad, Fraydoon, Nes, W. David, Littman, Dan R., Sanford-Burnham), VU-MED), CNRS-UMR), Ljubljana), and NYUSM). Identification of Natural ROR;#947; Ligands that Regulate the Development of Lymphoid Cells. United States: N. p., 2016. Web. doi:10.1016/j.cmet.2015.01.004.
Santori, Fabio R., Huang, Pengxiang, van de Pavert, Serge A., Douglass, Jr., Eugene F., Leaver, David J., Haubrich, Brad A., Keber, Rok, Lorbek, Gregor, Konijn, Tanja, Rosales, Brittany N., Rozman, Damjana, Horvat, Simon, Rahier, Alain, Mebius, Reina E., Rastinejad, Fraydoon, Nes, W. David, Littman, Dan R., Sanford-Burnham), VU-MED), CNRS-UMR), Ljubljana), & NYUSM). Identification of Natural ROR;#947; Ligands that Regulate the Development of Lymphoid Cells. United States. doi:10.1016/j.cmet.2015.01.004.
Santori, Fabio R., Huang, Pengxiang, van de Pavert, Serge A., Douglass, Jr., Eugene F., Leaver, David J., Haubrich, Brad A., Keber, Rok, Lorbek, Gregor, Konijn, Tanja, Rosales, Brittany N., Rozman, Damjana, Horvat, Simon, Rahier, Alain, Mebius, Reina E., Rastinejad, Fraydoon, Nes, W. David, Littman, Dan R., Sanford-Burnham), VU-MED), CNRS-UMR), Ljubljana), and NYUSM). 2016. "Identification of Natural ROR;#947; Ligands that Regulate the Development of Lymphoid Cells". United States. doi:10.1016/j.cmet.2015.01.004.
@article{osti_1222811,
title = {Identification of Natural ROR;#947; Ligands that Regulate the Development of Lymphoid Cells},
author = {Santori, Fabio R. and Huang, Pengxiang and van de Pavert, Serge A. and Douglass, Jr., Eugene F. and Leaver, David J. and Haubrich, Brad A. and Keber, Rok and Lorbek, Gregor and Konijn, Tanja and Rosales, Brittany N. and Rozman, Damjana and Horvat, Simon and Rahier, Alain and Mebius, Reina E. and Rastinejad, Fraydoon and Nes, W. David and Littman, Dan R. and Sanford-Burnham) and VU-MED) and CNRS-UMR) and Ljubljana) and NYUSM)},
abstractNote = {},
doi = {10.1016/j.cmet.2015.01.004},
journal = {Cell Metab.},
number = (3) ; 02, 2015,
volume = 21,
place = {United States},
year = 2016,
month = 7
}
  • The suppressor activity of the spleen cells from bone marrow chimeras prepared with total-lymphoid irradiation was analyzed in vitro. The chimeric spleen cells lacked responsiveness to host-type, but not to third-party, antigens in the mixed-leukocyte reaction (MLR) as judged by (3H)thymidine incorporation and the generation of cytolytic cells. When the donor-type chimeric spleen cells were used as cocultured cells in the MLR, modest nonspecific suppression of (3H)thymidine incorporation and potent antigen-specific suppression of the generation of the cytolytic cells was observed. The donor-type suppressor cells may play an important role in preventing graft-versus-host disease in vivo.
  • The authors studied the surface markers of suppressor cells of the mixed leukocyte reaction (MLR) that are transiently present in the spleens of neonatal mice after birth and of adult mice after total lymphoid irradiation (TLI). Approximately 80% of the mononuclear cells in the spleen, within the first few days after birth or after TLI, express neither the Thy-1 antigen nor surface immunoglobulin (Ig). After 30 days, less than 20% of mononuclear cells bear this null phenotype. With the use of the panning technique, they showed that the suppressors of the MLR are confined to the null cell population. Themore » null suppressor cells are not macrophages because they did not carry macrophage markers identified by the monoclonal antibodies anti-MAC-1 and F4/80. In addition, the suppressor cells did not stain for nonspecific esterase and did not adhere firmly to plastic or glass. Spleen cells from TLI-treated mice maintained their suppressive capacity after culture in vitro for 6 to 8 wk. The cultured suppressor cells did not develop mature T cell, B cell, or macrophage markers during this time interval. Thus, the suppressor cells did not appear to be precursors of the latter cells. The suppressor cells are similar to NK cells in that both are found in the absence of antigenic challenge, lack antigen specificity, and bear the null surface phenotype. Thus, they have termed the former cells natural suppressor (NS) cells.« less
  • Although several laboratories have shown that the appearance of naturally occurring suppressor cells in the spleens of neonatal or irradiated mice is temporarily related to the ease of induction of tolerance, the characteristics of these cells and their regulatory functions have been poorly understood until recently. The experimental data reviewed herein suggests that these cells are related to NK cells with regard to surface phenotype but differ with regard to function. The natural suppressor (NS) cells appear only briefly during the early maturation of the lymphoid tissues but can be induced in adults by manipulation of the lymphoid tissues withmore » certain treatment regimens such as total lymphoid irradiation (TLI). In addition, the NS cells can be propagated and cloned in long-term tissue culture, thereby allowing a more detailed investigation of their properties. The cells have the unique feature of inhibiting the antigen-specific cytolytic arm of alloreactive immune responses but leaving intact the antigen-specific suppressive arm. In this way, alloreactions in the regulatory milieu of NS cells generate large numbers of antigen-specific suppressor cells that can maintain tolerance in vivo. Thus the NS cells may play an important role in the development of host-vs-graft and graft-vs-host tolerance in allogeneic bone marrow chimeras during the ''window'' of tolerance in which neonate and TLI-treated mice accept the infused allogeneic cells. 70 references.« less
  • BALB/c mice treated with total lymphoid irradiation (TLI) develop non-antigen-specific suppressor cells of the adoptive secondary antibody response and of the mixed leukocyte reaction. Suppressors of the adoptive anti-DNP response were eliminated by incubation of spleen cells with anti-Thy-1.2 or anti-thymus-leukemia (TL) antiserum and complement before cell transfer. Thymectomy before TLI prevented the appearance of the latter suppressor cells. On the other hand, suppressors of the MLR were eliminated by incubation of spleen cells with anti-Thy-1.2 but not anti-TL antiserum and complement. Thymectomy before TLI did not prevent their subsequent development. Thus, two subpopulations of suppressor T cells that differmore » in the expression of the TL surface antigen, dependence on the presence of the thymus, and in regulatory functions develop after TLI. The TL+, thymus-dependent cell suppresses the adoptive antibody response, and the TL-, thymus-independent cell suppresses the MLR.« less