GLO-Roots: an imaging platform enabling multidimensional characterization of soil-grown root systems

Rellán-Álvarez, Rubén; Lobet, Guillaume; Lindner, Heike; Pradier, Pierre-Luc; Sebastian, Jose; Yee, Muh-Ching; Geng, Yu; Trontin, Charlotte; LaRue, Therese; Schrager-Lavelle, Amanda; Haney, Cara H.; Nieu, Rita; Maloof, Julin; Vogel, John P.; Dinneny, José R.

doi:10.7554/eLife.07597

Title: GLO-Roots: an imaging platform enabling multidimensional characterization of soil-grown root systems

Journal Article · Wed Aug 19 00:00:00 EDT 2015 · eLife

DOI:https://doi.org/10.7554/eLife.07597· OSTI ID:1222496

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; Geng, Yu;

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; Nieu, Rita;

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Root systems develop different root types that individually sense cues from their local environment and integrate this information with systemic signals. This complex multi-dimensional amalgam of inputs enables continuous adjustment of root growth rates, direction, and metabolic activity that define a dynamic physical network. Current methods for analyzing root biology balance physiological relevance with imaging capability. To bridge this divide, we developed an integrated-imaging system called Growth and Luminescence Observatory for Roots (GLO-Roots) that uses luminescence-based reporters to enable studies of root architecture and gene expression patterns in soil-grown, light-shielded roots. We have developed image analysis algorithms that allow the spatial integration of soil properties, gene expression, and root system architecture traits. We propose GLO-Roots as a system that has great utility in presenting environmental stimuli to roots in ways that evoke natural adaptive responses and in providing tools for studying the multi-dimensional nature of such processes.

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Research Organization:: Carnegie Inst. of Science, Stanford, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0008769; AI02-07ER64452; SC0001526

OSTI ID:: 1222496

Alternate ID(s):: OSTI ID: 1222497; OSTI ID: 1253189

Journal Information:: eLife, Journal Name: eLife Vol. 4; ISSN 2050-084X

Publisher:: eLife Sciences Publications, Ltd.Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 151 works

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