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Title: Capturing the response of Clostridium acetobutylicum to chemical stressors using a regulated genome-scale metabolic model

Abstract

Clostridia are anaerobic Gram-positive Firmicutes containing broad and flexible systems for substrate utilization, which have been used successfully to produce a range of industrial compounds. Clostridium acetobutylicum has been used to produce butanol on an industrial scale through acetone-butanol-ethanol (ABE) fermentation. A genome-scale metabolic (GSM) model is a powerful tool for understanding the metabolic capacities of an organism and developing metabolic engineering strategies for strain development. The integration of stress related specific transcriptomics information with the GSM model provides opportunities for elucidating the focal points of regulation.

Authors:
 [1];  [1];  [2];  [2];  [1]
  1. Pennsylvania State Univ., University Park, PA (United States)
  2. Delaware Biotechnology Institute, Newark, DE (United States); Univ. of Delaware, Newark, DE (United States)
Publication Date:
Research Org.:
Pennsylvania State Univ., University Park, PA (United States); University of Delaware
Sponsoring Org.:
USDOE
OSTI Identifier:
1221631
Alternate Identifier(s):
OSTI ID: 1454689
Grant/Contract Number:  
SC0007092; sc0007092
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; Clostridium acetobutylicum; CoreReg; regulation; genome-scale metabolic model

Citation Formats

Dash, Satyakam, Mueller, Thomas J., Venkataramanan, Keerthi P., Papoutsakis, Eleftherios T., and Maranas, Costas D.. Capturing the response of Clostridium acetobutylicum to chemical stressors using a regulated genome-scale metabolic model. United States: N. p., 2014. Web. doi:10.1186/s13068-014-0144-4.
Dash, Satyakam, Mueller, Thomas J., Venkataramanan, Keerthi P., Papoutsakis, Eleftherios T., & Maranas, Costas D.. Capturing the response of Clostridium acetobutylicum to chemical stressors using a regulated genome-scale metabolic model. United States. doi:10.1186/s13068-014-0144-4.
Dash, Satyakam, Mueller, Thomas J., Venkataramanan, Keerthi P., Papoutsakis, Eleftherios T., and Maranas, Costas D.. Tue . "Capturing the response of Clostridium acetobutylicum to chemical stressors using a regulated genome-scale metabolic model". United States. doi:10.1186/s13068-014-0144-4. https://www.osti.gov/servlets/purl/1221631.
@article{osti_1221631,
title = {Capturing the response of Clostridium acetobutylicum to chemical stressors using a regulated genome-scale metabolic model},
author = {Dash, Satyakam and Mueller, Thomas J. and Venkataramanan, Keerthi P. and Papoutsakis, Eleftherios T. and Maranas, Costas D.},
abstractNote = {Clostridia are anaerobic Gram-positive Firmicutes containing broad and flexible systems for substrate utilization, which have been used successfully to produce a range of industrial compounds. Clostridium acetobutylicum has been used to produce butanol on an industrial scale through acetone-butanol-ethanol (ABE) fermentation. A genome-scale metabolic (GSM) model is a powerful tool for understanding the metabolic capacities of an organism and developing metabolic engineering strategies for strain development. The integration of stress related specific transcriptomics information with the GSM model provides opportunities for elucidating the focal points of regulation.},
doi = {10.1186/s13068-014-0144-4},
journal = {Biotechnology for Biofuels},
issn = {1754-6834},
number = 1,
volume = 7,
place = {United States},
year = {2014},
month = {10}
}

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Free Publicly Available Full Text
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Cited by: 22 works
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Works referenced in this record:

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  • Tusher, V. G.; Tibshirani, R.; Chu, G.
  • Proceedings of the National Academy of Sciences, Vol. 98, Issue 9, p. 5116-5121
  • DOI: 10.1073/pnas.091062498

Engineering solventogenic clostridia
journal, October 2008