skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: An Intrinsically Disordered Peptide from Ebola Virus VP35 Controls Viral RNA Synthesis by Modulating Nucleoprotein-RNA Interactions

Journal Article · · Cell Reports

Research Organization:
Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Organization:
USDOE Office of Science (SC)
OSTI ID:
1221426
Journal Information:
Cell Reports, Vol. 11, Issue (3) ; 04, 2015
Country of Publication:
United States
Language:
ENGLISH

References (45)

The Ebola Virus VP35 Protein Inhibits Activation of Interferon Regulatory Factor 3 journal July 2003
Structural dissection of Ebola virus and its assembly determinants using cryo-electron tomography journal February 2012
TopDraw: a sketchpad for protein structure topology cartoons journal January 2003
Measurement errors and their consequences in protein crystallography journal October 2003
Diffraction data analysis in the presence of radiation damage journal March 2010
The CCP4 suite programs for protein crystallography journal September 1994
The Buccaneer software for automated model building. 1. Tracing protein chains journal August 2006
The structure of the C-terminal domain of the Zaire ebolavirus nucleoprotein journal August 2014
Ebola protein analyses for the determination of genetic organization journal September 1993
Coot model-building tools for molecular graphics journal November 2004
ESPript/ENDscript: extracting and rendering sequence and 3D information from atomic structures of proteins journal July 2003
A C-terminal basic amino acid motif of Zaire ebolavirus VP35 is essential for type I interferon antagonism and displays high identity with the RNA-binding domain of another interferon antagonist, the NS1 protein of influenza A virus journal October 2004
Enhancing the functional annotation of PDB structures in PDBsum using key figures extracted from the literature journal March 2007
LigPlot+: Multiple Ligand–Protein Interaction Diagrams for Drug Discovery journal October 2011
Structure of the Ebola VP35 interferon inhibitory domain journal January 2009
Ebolavirus VP35 is a multifunctional virulence factor journal November 2010
Structural basis for dsRNA recognition and interferon antagonism by Ebola VP35 journal January 2010
The N 0 -binding region of the vesicular stomatitis virus phosphoprotein is globally disordered but contains transient α-helices: Transient Structure in the N 0 -Binding Region of VSV P journal February 2011
Structure of the Vesicular Stomatitis Virus N0-P Complex journal September 2011
Mutual Antagonism between the Ebola Virus VP35 Protein and the RIG-I Activator PACT Determines Infection Outcome journal July 2013
Complex formation with vesicular stomatitis virus phosphoprotein NS prevents binding of nucleocapsid protein N to nonspecific RNA journal August 1988
HKL -3000: the integration of data reduction and structure solution – from diffraction images to an initial model in minutes journal July 2006
Homo-Oligomerization of Marburgvirus VP35 Is Essential for Its Function in Replication and Transcription journal December 2005
Filovirus replication and transcription journal March 2007
Comparison of the Transcription and Replication Strategies of Marburg Virus and Ebola Virus by Using Artificial Replication Systems journal March 1999
Refinement of Macromolecular Structures by the Maximum-Likelihood Method journal May 1997
Regions in Ebola Virus VP24 That Are Important for Nucleocapsid Formation journal November 2007
Characterization of the Ebola virus nucleoprotein-RNA complex journal February 2010
The Importance of the NP: VP35 Ratio in Ebola Virus Nucleocapsid Formation journal November 2011
[20] Processing of X-ray diffraction data collected in oscillation mode book January 1997
Multiparametric scaling of diffraction intensities journal April 2003
Basic Residues within the Ebolavirus VP35 Protein Are Required for Its Viral Polymerase Cofactor Function journal August 2010
Homo-oligomerization facilitates the interferon-antagonist activity of the ebolavirus VP35 protein journal October 2005
Identification and analysis of ebola virus messenger RNA journal April 1987
Sequence‐Based Human Leukocyte Antigen–B Typing of Patients Infected with Ebola Virus in Uganda in 2000: Identification of Alleles Associated with Fatal and Nonfatal Disease Outcomes journal November 2007
A short history of SHELX journal December 2007
A Filovirus-Unique Region of Ebola Virus Nucleoprotein Confers Aberrant Migration and Mediates Its Incorporation into Virions journal April 2008
EMAN2: An extensible image processing suite for electron microscopy journal January 2007
SOLVE and RESOLVE: automated structure solution, density modification and model building journal November 2003
The L–VP35 and L–L interaction domains reside in the amino terminus of the Ebola virus L protein and are potential targets for antivirals journal July 2013
Structure of Nipah virus unassembled nucleoprotein in complex with its viral chaperone journal August 2014
Miscellaneous Algorithms for Density Modification journal July 1998
Ebola Virus VP35 Antagonizes PKR Activity through Its C-Terminal Interferon Inhibitory Domain journal June 2009
Functional Mapping of the Nucleoprotein of Ebola Virus journal March 2006
Deep Sequencing Identifies Noncanonical Editing of Ebola and Marburg Virus RNAs in Infected Cells journal November 2014

Cited By (39)

The structure of the nucleoprotein of Influenza D shows that all Orthomyxoviridae nucleoproteins have a similar NPCORE, with or without a NPTAIL for nuclear transport journal January 2019
Predictive and comparative analysis of Ebolavirus proteins journal July 2015
Sudan Ebolavirus VP35-NP Crystal Structure Reveals a Potential Target for Pan-Filovirus Treatment journal July 2019
Efficient ligand discovery from natural herbs by integrating virtual screening, affinity mass spectrometry and targeted metabolomics journal January 2019
Cryo-EM structure of the Ebola virus nucleoprotein–RNA complex at 3.6 Å resolution journal October 2018
Structure and assembly of the Ebola virus nucleocapsid journal November 2017
Cryo-EM structure of the Ebola virus nucleoprotein–RNA complex journal April 2019
How order and disorder within paramyxoviral nucleoproteins and phosphoproteins orchestrate the molecular interplay of transcription and replication journal June 2017
Modulation of Re-initiation of Measles Virus Transcription at Intergenic Regions by PXD to NTAIL Binding Strength journal December 2016
Conformational Selection of a Polyproline Peptide by Ebola Virus VP30 journal October 2018
Recent advances in marburgvirus research journal January 2019
Structure of the Paramyxovirus Parainfluenza Virus 5 Nucleoprotein in Complex with an Amino-Terminal Peptide of the Phosphoprotein journal December 2017
A simulation investigation on interaction mechanism between Ebola nucleoprotein and VP35 peptide text January 2017
The Ebola Virus Nucleoprotein Recruits the Nuclear RNA Export Factor NXF1 into Inclusion Bodies to Facilitate Viral Protein Expression journal January 2020
Binding of RNA by the Nucleoproteins of Influenza Viruses A and B journal September 2016
Human transbodies that interfere with the functions of Ebola virus VP35 protein in genome replication and transcription and innate immune antagonism journal December 2018
The Ebola Virus VP30-NP Interaction Is a Regulator of Viral RNA Synthesis journal October 2016
Perspectives towards antiviral drug discovery against Ebola virus journal August 2019
Cooperative changes in solvent exposure identify cryptic pockets, conformational switches, and allosteric coupling journal September 2018
Structure of the Ebola virus nucleoprotein – RNA complex
  • Kirchdoerfer, Robert N.; Saphire, Erica Ollmann; Ward, Andrew B.
  • Acta Crystallographica Section F Structural Biology Communications https://doi.org/10.1101/548313
journal February 2019
Ebolaviruses: New roles for old proteins journal May 2018
Role of Antibodies in Protection Against Ebola Virus in Nonhuman Primates Immunized With Three Vaccine Platforms journal June 2018
Reporter Assays for Ebola Virus Nucleoprotein Oligomerization, Virion-Like Particle Budding, and Minigenome Activity Reveal the Importance of Nucleoprotein Amino Acid Position 111 journal January 2020
Global phosphoproteomic analysis of Ebola virions reveals a novel role for VP35 phosphorylation-dependent regulation of genome transcription journal September 2019
A water-stable metal–organic framework of a zwitterionic carboxylate with dysprosium: a sensing platform for Ebolavirus RNA sequences journal January 2016
Cooperative changes in solvent exposure identify cryptic pockets, conformational switches and allosteric coupling journal July 2019
A simulation investigation on interaction mechanism between Ebola nucleoprotein and VP35 peptide text January 2017
A simulation investigation on interaction mechanism between Ebola nucleoprotein and VP35 peptide journal April 2017
Insight into the Ebola virus nucleocapsid assembly mechanism: crystal structure of Ebola virus nucleoprotein core domain at 1.8 Å resolution journal April 2015
In vitro assembly of Ebola virus nucleocapsid-like complex expressed in E. coli journal September 2016
Ebola Viral Glycoprotein Bound to Its Endosomal Receptor Niemann-Pick C1 journal January 2016
Assembly of the Ebola Virus Nucleoprotein from a Chaperoned VP35 Complex journal July 2015
Ebola virus VP24 interacts with NP to facilitate nucleocapsid assembly and genome packaging journal August 2017
Novel Chemical Ligands to Ebola Virus and Marburg Virus Nucleoproteins Identified by Combining Affinity Mass Spectrometry and Metabolomics Approaches journal July 2016
The lifecycle of the Ebola virus in host cells journal June 2017
The Nucleoprotein and Phosphoprotein of Measles Virus journal August 2019
On the Helix Propensity in Generalized Born Solvent Descriptions of Modeling the Dark Proteome journal January 2017
Marburg Virus Reverse Genetics Systems journal June 2016
Nucleocapsid assembly in pneumoviruses is regulated by conformational switching of the N protein journal February 2016

Similar Records

An intrinsically disordered peptide from Ebola virus VP35 controls viral RNA synthesis by modulating nucleoprotein-RNA interactions
Journal Article · Wed Apr 01 00:00:00 EDT 2015 · Cell Reports · OSTI ID:1221426

Ebola virus VP30 and nucleoprotein interactions modulate viral RNA synthesis
Journal Article · Thu Jun 08 00:00:00 EDT 2017 · Nature Communications · OSTI ID:1221426

Ebola Virus VP35 Interaction with Dynein LC8 Regulates Viral RNA Synthesis
Journal Article · Fri Apr 03 00:00:00 EDT 2015 · Journal of Virology · OSTI ID:1221426

Related Subjects