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Title: Distinct roles of the photosystem II protein PsbS and zeaxanthin in the regulation of light harvesting in plants revealed by fluorescence lifetime snapshots

Journal Article · · Proceedings of the National Academy of Sciences of the United States of America
 [1];  [1];  [1];  [1];  [1];  [1];  [1]
  1. Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
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The photosystem II (PSII) protein PsbS and the enzyme violaxanthin deepoxidase (VDE) are known to influence the dynamics of energy-dependent quenching (qE), the component of nonphotochemical quenching (NPQ) that allows plants to respond to fast fluctuations in light intensity. Although the absence of PsbS and VDE has been shown to change the amount of quenching, there have not been any measurements that can detect whether the presence of these proteins alters the type of quenching that occurs. The chlorophyll fluorescence lifetime probes the excitedstate chlorophyll relaxation dynamics and can be used to determine the amount of quenching as well as whether two different genotypes with the same amount of NPQ have similar dynamics of excited-state chlorophyll relaxation. We measured the fluorescence lifetimes on whole leaves of Arabidopsis thaliana throughout the induction and relaxation of NPQ for wild type and the qE mutants, npq4, which lacks PsbS; npq1, which lacks VDE and cannot convert violaxanthin to zeaxanthin; and npq1 npq4, which lacks both VDE and PsbS. These measurements show that although PsbS changes the amount of quenching and the rate at which quenching turns on, it does not affect the relaxation dynamics of excited chlorophyll during quenching. In addition, the data suggest that PsbS responds not only to pH but also to the across the thylakoid membrane. In contrast, the presence of VDE, which is necessary for the accumulation of zeaxanthin, affects the excited-state chlorophyll relaxation dynamics.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1221215
Alternate ID(s):
OSTI ID: 1407266
Journal Information:
Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 49; ISSN 0027-8424
Publisher:
National Academy of Sciences, Washington, DC (United States)Copyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 43 works
Citation information provided by
Web of Science

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Cited By (16)

Photoprotection and growth under different lights of Arabidopsis single and double mutants for energy dissipation (npq4) and state transitions (pph1) journal March 2019
Time-resolved fluorescence measurements on leaves: principles and recent developments journal November 2018
Dynamics of regulated YNPQ and non-regulated YNO energy dissipation in sunflower leaves exposed to sinusoidal lights journal March 2019
Crystal structures of the PsbS protein essential for photoprotection in plants journal August 2015
Efficient photosynthesis in dynamic light environments: a chloroplast's perspective journal October 2019
Chlorophyll–carotenoid excitation energy transfer and charge transfer in Nannochloropsis oceanica for the regulation of photosynthesis journal February 2019
The PSI–PSII Megacomplex in Green Plants journal February 2019
Models and mechanisms of the rapidly reversible regulation of photosynthetic light harvesting journal April 2019
Leaves of isoprene‐emitting tobacco plants maintain PSII stability at high temperatures journal May 2019
Photosystem II 22kDa protein level ‐ a prerequisite for excess light‐inducible memory, cross‐tolerance to UV‐C and regulation of electrical signalling journal December 2019
Quantitative Effects of Pigmentation on the Re‐absorption of Chlorophyll a Fluorescence and Energy Partitioning in Leaves journal July 2019
Expression and functional analysis of two PsbS genes in bamboo ( Phyllostachys edulis ) journal March 2018
Multilevel regulation of non‐photochemical quenching and state transitions by chloroplast NADPH‐dependent thioredoxin reductase journal February 2019
Xanthophyll Cycle Activity in Two Prominent Arctic Shrub Species journal May 2017
Genetic basis of photosynthetic responses to cold in two locally adapted populations of Arabidopsis thaliana journal December 2017
The Plastid Lipocalin LCNP Is Required for Sustained Photoprotective Energy Dissipation in Arabidopsis journal December 2017

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59 BASIC BIOLOGICAL SCIENCES
PsbS
nonphotochemical quenching
fluorescence lifetime
carotenoids
photosystem II