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Title: Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius

Abstract

In microorganisms, Ion Torrent sequencing technology has been proved to be useful in whole-genome sequencing of bacterial genomes (5 Mbp). In our study, for the first time we used this technology to perform a resequencing approach in a whole fungal genome (36 Mbp), a non-ochratoxin A producing strain of Aspergillus carbonarius. Ochratoxin A (OTA) is a potent nephrotoxin which is found mainly in cereals and their products, but it also occurs in a variety of common foods and beverages. Due to the fact that this strain does not produce OTA, we focused some of the bioinformatics analyses in genes involved in OTA biosynthesis, using a reference genome of an OTA producing strain of the same species. This study revealed that in the atoxigenic strain there is a high accumulation of nonsense and missense mutations in several genes. Importantly, a two fold increase in gene mutation ratio was observed in PKS and NRPS encoding genes which are suggested to be involved in OTA biosynthesis.

Authors:
 [1];  [2];  [1];  [1];  [2];  [1]
  1. Univ. Autònoma de Barcelona, Bellaterra, Catalonia (Spain)
  2. Sequentia Biotech SL, Barcelona, Catalonia (Spain)
Publication Date:
Research Org.:
Universitat Autònoma de Barcelona, Bellaterra, Catalonia (Spain)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Contributing Org.:
Joint Genome Institute, Walnut Creek, CA (United States)
OSTI Identifier:
1215424
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 5; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; fungal genomics; gene regulatory networks

Citation Formats

Cabañes, F. Javier, Sanseverino, Walter, Castellá, Gemma, Bragulat, M. Rosa, Cigliano, Riccardo Aiese, and Sánchez, Armand. Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius. United States: N. p., 2015. Web. doi:10.1038/srep09086.
Cabañes, F. Javier, Sanseverino, Walter, Castellá, Gemma, Bragulat, M. Rosa, Cigliano, Riccardo Aiese, & Sánchez, Armand. Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius. United States. doi:10.1038/srep09086.
Cabañes, F. Javier, Sanseverino, Walter, Castellá, Gemma, Bragulat, M. Rosa, Cigliano, Riccardo Aiese, and Sánchez, Armand. Fri . "Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius". United States. doi:10.1038/srep09086. https://www.osti.gov/servlets/purl/1215424.
@article{osti_1215424,
title = {Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius},
author = {Cabañes, F. Javier and Sanseverino, Walter and Castellá, Gemma and Bragulat, M. Rosa and Cigliano, Riccardo Aiese and Sánchez, Armand},
abstractNote = {In microorganisms, Ion Torrent sequencing technology has been proved to be useful in whole-genome sequencing of bacterial genomes (5 Mbp). In our study, for the first time we used this technology to perform a resequencing approach in a whole fungal genome (36 Mbp), a non-ochratoxin A producing strain of Aspergillus carbonarius. Ochratoxin A (OTA) is a potent nephrotoxin which is found mainly in cereals and their products, but it also occurs in a variety of common foods and beverages. Due to the fact that this strain does not produce OTA, we focused some of the bioinformatics analyses in genes involved in OTA biosynthesis, using a reference genome of an OTA producing strain of the same species. This study revealed that in the atoxigenic strain there is a high accumulation of nonsense and missense mutations in several genes. Importantly, a two fold increase in gene mutation ratio was observed in PKS and NRPS encoding genes which are suggested to be involved in OTA biosynthesis.},
doi = {10.1038/srep09086},
journal = {Scientific Reports},
number = ,
volume = 5,
place = {United States},
year = {Fri Mar 13 00:00:00 EDT 2015},
month = {Fri Mar 13 00:00:00 EDT 2015}
}

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Cited by: 2 works
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