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Title: Nano-immunoassay with improved performance for detection of cancer biomarkers

Abstract

Nano-immunoassay utilizing surface-enhanced Raman scattering (SERS) effect is a promising analytical technique for the early detection of cancer. In its current standing the assay is capable of discriminating samples of healthy individuals from samples of pancreatic cancer patients. Further improvements in sensitivity and reproducibility will extend practical applications of the SERS-based detection platforms to wider range of problems. In this report, we discuss several strategies designed to improve performance of the SERS-based detection system. We demonstrate that reproducibility of the platform is enhanced by using atomically smooth mica surface as a template for preparation of capture surface in SERS sandwich immunoassay. Furthermore, the assay's stability and sensitivity can be further improved by using either polymer or graphene monolayer as a thin protective layer applied on top of the assay addresses. The protective layer renders the signal to be more stable against photo-induced damage and carbonaceous contamination.

Authors:
 [1];  [1];  [1];  [2];  [3];  [1];  [1];  [1]
  1. Univ. of Nebraska Medical Center, Omaha, NE (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. Iowa State Univ., Ames, IA (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1214460
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Nanomedicine: Nanotechnologoy, Biology, and Medicine
Additional Journal Information:
Journal Volume: 11; Journal Issue: 1; Journal ID: ISSN 1549-9634
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Krasnoslobodtsev, Alexey V., Torres, Maria P., Kaur, Sukhwinder, Vlassiouk, Ivan V., Lipert, Robert J., Jain, Maneesh, Batra, Surinder K., and Lyubchenko, Yuri L. Nano-immunoassay with improved performance for detection of cancer biomarkers. United States: N. p., 2015. Web. doi:10.1016/j.nano.2014.08.012.
Krasnoslobodtsev, Alexey V., Torres, Maria P., Kaur, Sukhwinder, Vlassiouk, Ivan V., Lipert, Robert J., Jain, Maneesh, Batra, Surinder K., & Lyubchenko, Yuri L. Nano-immunoassay with improved performance for detection of cancer biomarkers. United States. doi:10.1016/j.nano.2014.08.012.
Krasnoslobodtsev, Alexey V., Torres, Maria P., Kaur, Sukhwinder, Vlassiouk, Ivan V., Lipert, Robert J., Jain, Maneesh, Batra, Surinder K., and Lyubchenko, Yuri L. Thu . "Nano-immunoassay with improved performance for detection of cancer biomarkers". United States. doi:10.1016/j.nano.2014.08.012. https://www.osti.gov/servlets/purl/1214460.
@article{osti_1214460,
title = {Nano-immunoassay with improved performance for detection of cancer biomarkers},
author = {Krasnoslobodtsev, Alexey V. and Torres, Maria P. and Kaur, Sukhwinder and Vlassiouk, Ivan V. and Lipert, Robert J. and Jain, Maneesh and Batra, Surinder K. and Lyubchenko, Yuri L.},
abstractNote = {Nano-immunoassay utilizing surface-enhanced Raman scattering (SERS) effect is a promising analytical technique for the early detection of cancer. In its current standing the assay is capable of discriminating samples of healthy individuals from samples of pancreatic cancer patients. Further improvements in sensitivity and reproducibility will extend practical applications of the SERS-based detection platforms to wider range of problems. In this report, we discuss several strategies designed to improve performance of the SERS-based detection system. We demonstrate that reproducibility of the platform is enhanced by using atomically smooth mica surface as a template for preparation of capture surface in SERS sandwich immunoassay. Furthermore, the assay's stability and sensitivity can be further improved by using either polymer or graphene monolayer as a thin protective layer applied on top of the assay addresses. The protective layer renders the signal to be more stable against photo-induced damage and carbonaceous contamination.},
doi = {10.1016/j.nano.2014.08.012},
journal = {Nanomedicine: Nanotechnologoy, Biology, and Medicine},
number = 1,
volume = 11,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 2015},
month = {Thu Jan 01 00:00:00 EST 2015}
}

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Cited by: 8 works
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