X-ray crystal structures of native HIV-1 capsid protein reveal conformational variability

Gres, Anna T.; Kirby, Karen A.; KewalRamani, Vineet N.; Tanner, John J.; Pornillos, Owen; Sarafianos, Stefan G.

doi:10.1126/science.aaa5936

Title: X-ray crystal structures of native HIV-1 capsid protein reveal conformational variability

Journal Article · Thu Jun 04 00:00:00 EDT 2015 · Science

DOI:https://doi.org/10.1126/science.aaa5936· OSTI ID:1214294

Gres, Anna T. ^[1]; Kirby, Karen A. ^[2]; KewalRamani, Vineet N. ^[3]; Tanner, John J. ^[4]; Pornillos, Owen ^[5]; Sarafianos, Stefan G. ^[6]

Univ. of Missouri, Columbia, MO (United States). Christopher S. Bond Life Sciences Center. Dept. of Chemistry
Univ. of Missouri, Columbia, MO (United States). Christopher S. Bond Life Sciences Center. School of Medicine. Dept. of Molecular Microbiology and Immunology
National Cancer Inst., Frederick, MD (United States). Center for Cancer Research. Basic Research Lab.
Univ. of Missouri, Columbia, MO (United States). Dept. of Chemistry. Dept. of Biochemistry
Univ. of Virginia, Charlottesville, VA (United States). School of Medicine. Dept. of Molecular Physiology and Biological Physics
Univ. of Missouri, Columbia, MO (United States). Christopher S. Bond Life Sciences Center. School of Medicine. Dept. of Molecular Microbiology and Immunology. Dept. of Biochemistry

The detailed molecular interactions between native HIV-1 capsid protein (CA) hexamers that shield the viral genome and proteins have been elusive. In this paper, we report crystal structures describing interactions between CA monomers related by sixfold symmetry within hexamers (intrahexamer) and threefold and twofold symmetry between neighboring hexamers (interhexamer). The structures describe how CA builds hexagonal lattices, the foundation of mature capsids. Lattice structure depends on an adaptable hydration layer modulating interactions among CA molecules. Disruption of this layer alters interhexamer interfaces, highlighting an inherent structural variability. A CA-targeting antiviral affects capsid stability by binding across CA molecules and subtly altering interhexamer interfaces remote to the ligand-binding site. Finally, inherent structural plasticity, hydration layer rearrangement, and effector binding affect capsid stability and have functional implications for the retroviral life cycle.

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Research Organization:: Univ. of Missouri, Columbia, MO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Inst. of Health (NIH) (United States)

Contributing Organization:: National Cancer Inst., Frederick, MD (United States); Univ. of Virginia, Charlottesville, VA (United States)

Grant/Contract Number:: AC02-05CH11231; AI112417; AI120860; GM103368; AI076119; AI099284; AI100890; GM066087

OSTI ID:: 1214294

Journal Information:: Science, Vol. 349, Issue 6243; ISSN 0036-8075

Publisher:: AAASCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 169 works

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References (52)

X-Ray Structures of the Hexameric Building Block of the HIV Capsid Pornillos, Owen; Ganser-Pornillos, Barbie K.; Kelly, Brian N. Cell, Vol. 137, Issue 7 https://doi.org/10.1016/j.cell.2009.04.063	journal	June 2009
Mature HIV-1 capsid structure by cryo-electron microscopy and all-atom molecular dynamics Zhao, Gongpu; Perilla, Juan R.; Yufenyuy, Ernest L. Nature, Vol. 497, Issue 7451 https://doi.org/10.1038/nature12162	journal	May 2013
Atomic-level modelling of the HIV capsid Pornillos, Owen; Ganser-Pornillos, Barbie K.; Yeager, Mark Nature, Vol. 469, Issue 7330 https://doi.org/10.1038/nature09640	journal	January 2011
Structure and Dynamics of Full-Length HIV-1 Capsid Protein in Solution Deshmukh, Lalit; Schwieters, Charles D.; Grishaev, Alexander Journal of the American Chemical Society, Vol. 135, Issue 43 https://doi.org/10.1021/ja406246z	journal	October 2013
Structure of the HIV-1 Full-Length Capsid Protein in a Conformationally Trapped Unassembled State Induced by Small-Molecule Binding Du, Shoucheng; Betts, Laurie; Yang, Ruifeng Journal of Molecular Biology, Vol. 406, Issue 3 https://doi.org/10.1016/j.jmb.2010.11.027	journal	February 2011
The interdomain linker region of HIV-1 capsid protein is a critical determinant of proper core assembly and stability Jiang, Jiyang; Ablan, Sherimay D.; Derebail, Suchitra Virology, Vol. 421, Issue 2 https://doi.org/10.1016/j.virol.2011.09.012	journal	December 2011
Functional Surfaces of the Human Immunodeficiency Virus Type 1 Capsid Protein von Schwedler, U. K.; Stray, K. M.; Garrus, J. E. Journal of Virology, Vol. 77, Issue 9 https://doi.org/10.1128/JVI.77.9.5439-5450.2003	journal	May 2003
Assembly Properties of the Human Immunodeficiency Virus Type 1 CA Protein Ganser-Pornillos, B. K.; von Schwedler, U. K.; Stray, K. M. Journal of Virology, Vol. 78, Issue 5 https://doi.org/10.1128/JVI.78.5.2545-2552.2004	journal	February 2004
Extreme Genetic Fragility of the HIV-1 Capsid Rihn, Suzannah J.; Wilson, Sam J.; Loman, Nick J. PLoS Pathogens, Vol. 9, Issue 6 https://doi.org/10.1371/journal.ppat.1003461	journal	June 2013
Protease Cleavage Leads to Formation of Mature Trimer Interface in HIV-1 Capsid Meng, Xin; Zhao, Gongpu; Yufenyuy, Ernest PLoS Pathogens, Vol. 8, Issue 8 https://doi.org/10.1371/journal.ppat.1002886	journal	August 2012
Structure of the Carboxyl-Terminal Dimerization Domain of the HIV-1 Capsid Protein Gamble, T. R. Science, Vol. 278, Issue 5339 https://doi.org/10.1126/science.278.5339.849	journal	October 1997
Structure of the Antiviral Assembly Inhibitor CAP-1 Complex with the HIV-1 CA Protein Kelly, Brian N.; Kyere, Sampson; Kinde, Isaac Journal of Molecular Biology, Vol. 373, Issue 2 https://doi.org/10.1016/j.jmb.2007.07.070	journal	October 2007
Antiviral Inhibition of the HIV-1 Capsid Protein Tang, Chun; Loeliger, Erin; Kinde, Isaac Journal of Molecular Biology, Vol. 327, Issue 5 https://doi.org/10.1016/S0022-2836(03)00289-4	journal	April 2003
HIV Capsid is a Tractable Target for Small Molecule Therapeutic Intervention Blair, Wade S.; Pickford, Chris; Irving, Stephen L. PLoS Pathogens, Vol. 6, Issue 12 https://doi.org/10.1371/journal.ppat.1001220	journal	December 2010
Virtual screening based identification of novel small-molecule inhibitors targeted to the HIV-1 capsid Curreli, Francesca; Zhang, Hongtao; Zhang, Xihui Bioorganic & Medicinal Chemistry, Vol. 19, Issue 1 https://doi.org/10.1016/j.bmc.2010.11.045	journal	January 2011
The HIV-1 capsid protein C-terminal domain in complex with a virus assembly inhibitor Ternois, François; Sticht, Jana; Duquerroy, Stéphane Nature Structural & Molecular Biology, Vol. 12, Issue 8 https://doi.org/10.1038/nsmb967	journal	July 2005
Discovery of Novel Small-Molecule HIV-1 Replication Inhibitors That Stabilize Capsid Complexes Lamorte, Louie; Titolo, Steve; Lemke, Christopher T. Antimicrobial Agents and Chemotherapy, Vol. 57, Issue 10 https://doi.org/10.1128/AAC.00985-13	journal	July 2013
Host Cofactors and Pharmacologic Ligands Share an Essential Interface in HIV-1 Capsid That Is Lost upon Disassembly Price, Amanda J.; Jacques, David A.; McEwan, William A. PLoS Pathogens, Vol. 10, Issue 10 https://doi.org/10.1371/journal.ppat.1004459	journal	October 2014
Structural basis of HIV-1 capsid recognition by PF74 and CPSF6 Bhattacharya, Akash; Alam, Steven L.; Fricke, Thomas Proceedings of the National Academy of Sciences, Vol. 111, Issue 52 https://doi.org/10.1073/pnas.1419945112	journal	December 2014
BI-2 destabilizes HIV-1 cores during infection and Prevents Binding of CPSF6 to the HIV-1 Capsid Fricke, Thomas; Buffone, Cindy; Opp, Silvana Retrovirology, Vol. 11, Issue 1 https://doi.org/10.1186/s12977-014-0120-x	journal	December 2014
Quantitative microscopy of functional HIV post-entry complexes reveals association of replication with the viral capsid Peng, Ke; Muranyi, Walter; Glass, Bärbel eLife, Vol. 3 https://doi.org/10.7554/eLife.04114	journal	December 2014
Small-Molecule Inhibition of Human Immunodeficiency Virus Type 1 Infection by Virus Capsid Destabilization Shi, J.; Zhou, J.; Shah, V. B. Journal of Virology, Vol. 85, Issue 1 https://doi.org/10.1128/JVI.01406-10	journal	October 2010
Nucleoporin NUP153 Phenylalanine-Glycine Motifs Engage a Common Binding Pocket within the HIV-1 Capsid Protein to Mediate Lentiviral Infectivity Matreyek, Kenneth A.; Yücel, Sara S.; Li, Xiang PLoS Pathogens, Vol. 9, Issue 10 https://doi.org/10.1371/journal.ppat.1003693	journal	October 2013
Structure of Full-Length HIV-1 CA: A Model for the Mature Capsid Lattice Ganser-Pornillos, Barbie K.; Cheng, Anchi; Yeager, Mark Cell, Vol. 131, Issue 1 https://doi.org/10.1016/j.cell.2007.08.018	journal	October 2007
Structures of the HIV-1 capsid protein dimerization domain at 2.6 Å resolution Worthylake, David K.; Wang, Hui; Yoo, Sanghee Acta Crystallographica Section D Biological Crystallography, Vol. 55, Issue 1 https://doi.org/10.1107/S0907444998007689	journal	January 1999
Residues in the HIV-1 Capsid Assembly Inhibitor Binding Site Are Essential for Maintaining the Assembly-competent Quaternary Structure of the Capsid Protein Bartonova, Vanda; Igonet, Sébastien; Sticht, Jana Journal of Biological Chemistry, Vol. 283, Issue 46 https://doi.org/10.1074/jbc.M804230200	journal	September 2008
Implications for Viral Capsid Assembly from Crystal Structures of HIV-1 Gag ₁ _- ₂₇₈ and CA ^N ₁₃₃ _- ₂₇₈ ^† ^, ^‡ Kelly, Brian N.; Howard, Bruce R.; Wang, Hui Biochemistry, Vol. 45, Issue 38 https://doi.org/10.1021/bi060927x	journal	September 2006
Structure of the Amino-Terminal Core Domain of the HIV-1 Capsid Protein Gitti, R. K.; Lee, B. M.; Walker, J. Science, Vol. 273, Issue 5272 https://doi.org/10.1126/science.273.5272.231	journal	July 1996
Post-crystallization treatments for improving diffraction quality of protein crystals Heras, Begoña; Martin, Jennifer L. Acta Crystallographica Section D Biological Crystallography, Vol. 61, Issue 9 https://doi.org/10.1107/S0907444905019451	journal	August 2005
Mutation of Dileucine-Like Motifs in the Human Immunodeficiency Virus Type 1 Capsid Disrupts Virus Assembly, Gag-Gag Interactions, Gag-Membrane Binding, and Virion Maturation Joshi, A.; Nagashima, K.; Freed, E. O. Journal of Virology, Vol. 80, Issue 16 https://doi.org/10.1128/JVI.00355-06	journal	July 2006
HIV-1 B-subtype capsid protein: a characterization of amino acid’s conservation and its significant association with integrase signatures Dimonte, Salvatore; Babakir-Mina, Muhammed; Aquaro, Stefano Virus Genes, Vol. 48, Issue 3 https://doi.org/10.1007/s11262-014-1039-y	journal	January 2014
Conformational plasticity of a native retroviral capsid revealed by x-ray crystallography Obal, G.; Trajtenberg, F.; Carrión, F. Science, Vol. 349, Issue 6243 https://doi.org/10.1126/science.aaa5182	journal	June 2015
A model for cofactor use during HIV-1 reverse transcription and nuclear entry Hilditch, Laura; Towers, Greg J. Current Opinion in Virology, Vol. 4 https://doi.org/10.1016/j.coviro.2013.11.003	journal	February 2014
HIV-1 uncoating: connection to nuclear entry and regulation by host proteins Ambrose, Zandrea; Aiken, Christopher Virology, Vol. 454-455 https://doi.org/10.1016/j.virol.2014.02.004	journal	April 2014
Kinetic Analysis of the Role of Intersubunit Interactions in Human Immunodeficiency Virus Type 1 Capsid Protein Assembly In Vitro Lanman, J.; Sexton, J.; Sakalian, M. Journal of Virology, Vol. 76, Issue 14 https://doi.org/10.1128/JVI.76.14.6900-6908.2002	journal	July 2002
CPSF6 Defines a Conserved Capsid Interface that Modulates HIV-1 Replication Price, Amanda J.; Fletcher, Adam J.; Schaller, Torsten PLoS Pathogens, Vol. 8, Issue 8 https://doi.org/10.1371/journal.ppat.1002896	journal	August 2012
XDS Kabsch, Wolfgang Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2 https://doi.org/10.1107/S0907444909047337	journal	January 2010
HKL -3000: the integration of data reduction and structure solution – from diffraction images to an initial model in minutes Minor, Wladek; Cymborowski, Marcin; Otwinowski, Zbyszek Acta Crystallographica Section D Biological Crystallography, Vol. 62, Issue 8 https://doi.org/10.1107/S0907444906019949	journal	July 2006
Scaling and assessment of data quality Evans, Philip Acta Crystallographica Section D Biological Crystallography, Vol. 62, Issue 1, p. 72-82 https://doi.org/10.1107/S0907444905036693	journal	December 2005
PHENIX : building new software for automated crystallographic structure determination Adams, Paul D.; Grosse-Kunstleve, Ralf W.; Hung, Li-Wei Acta Crystallographica Section D Biological Crystallography, Vol. 58, Issue 11 https://doi.org/10.1107/S0907444902016657	journal	October 2002
Overview of the CCP 4 suite and current developments Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D. Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4 https://doi.org/10.1107/S0907444910045749	journal	March 2011
REFMAC 5 for the refinement of macromolecular crystal structures Murshudov, Garib N.; Skubák, Pavol; Lebedev, Andrey A. Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4 https://doi.org/10.1107/S0907444911001314	journal	March 2011
Coot model-building tools for molecular graphics Emsley, Paul; Cowtan, Kevin Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132 https://doi.org/10.1107/S0907444904019158	journal	November 2004
Formation of a Human Immunodeficiency Virus Type 1 Core of Optimal Stability Is Crucial for Viral Replication Forshey, B. M.; von Schwedler, U.; Sundquist, W. I. Journal of Virology, Vol. 76, Issue 11 https://doi.org/10.1128/JVI.76.11.5667-5677.2002	journal	June 2002
The effect of capsid mutations on HIV-1 uncoating Wacharapornin, Pathawut; Lauhakirti, Darat; Auewarakul, Prasert Virology, Vol. 358, Issue 1 https://doi.org/10.1016/j.virol.2006.08.031	journal	February 2007
Thermodynamic Dissection of a Low Affinity Protein-Protein Interface Involved in Human Immunodeficiency Virus Assembly del Álamo, Marta; Neira, José Luis; Mateu, Mauricio G. Journal of Biological Chemistry, Vol. 278, Issue 30 https://doi.org/10.1074/jbc.M304466200	journal	May 2003
Molecular recognition in the human immunodeficiency virus capsid and antiviral design Bocanegra, Rebeca; Rodríguez-Huete, Alicia; Fuertes, Miguel Ángel Virus Research, Vol. 169, Issue 2 https://doi.org/10.1016/j.virusres.2012.06.016	journal	November 2012
Structural Convergence between Cryo-EM and NMR Reveals Intersubunit Interactions Critical for HIV-1 Capsid Function Byeon, In-Ja L.; Meng, Xin; Jung, Jinwon Cell, Vol. 139, Issue 4 https://doi.org/10.1016/j.cell.2009.10.010	journal	November 2009
Fitness Costs of Mutations at the HIV-1 Capsid Hexamerization Interface Manocheewa, Siriphan; Swain, J. Victor; Lanxon-Cookson, Erinn PLoS ONE, Vol. 8, Issue 6 https://doi.org/10.1371/journal.pone.0066065	journal	June 2013
The oligomeric structures of plant cryptochromes Shao, Kai; Zhang, Xue; Li, Xu Nature Structural & Molecular Biology, Vol. 27, Issue 5 https://doi.org/10.1038/s41594-020-0420-x	journal	May 2020
Structure of the HIV-1 Full-Length Capsid Protein in a Conformationally Trapped Unassembled State Induced by Small-Molecule Binding Jinwoo, Ahn,; Jason, Concel,; I., Yeh, Joanne The University of North Carolina at Chapel Hill University Libraries https://doi.org/10.17615/2304-bd59	text	January 2011
Overview of the CCP4 suite and current developments. Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D. Apollo - University of Cambridge Repository https://doi.org/10.17863/cam.52322	text	January 2011

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Cryo-electron microscopy and single molecule fluorescent microscopy detect CD4 receptor induced HIV size expansion prior to cell entry Pham, Son; Tabarin, Thibault; Garvey, Megan Virology, Vol. 486 https://doi.org/10.1016/j.virol.2015.09.006	journal	December 2015
All-Atom Molecular Dynamics of Virus Capsids as Drug Targets Perilla, Juan R.; Hadden, Jodi A.; Goh, Boon Chong The Journal of Physical Chemistry Letters, Vol. 7, Issue 10 https://doi.org/10.1021/acs.jpclett.6b00517	journal	May 2016
In vitro assembly of the Rous Sarcoma Virus capsid protein into hexamer tubes at physiological temperature Jaballah, Soumeya A.; Bailey, Graham D.; Desfosses, Ambroise Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/s41598-017-02060-0	journal	June 2017
Structure of FIV capsid C-terminal domain demonstrates lentiviral evasion of genetic fragility by coevolved substitutions Khwaja, Aya; Galilee, Meytal; Marx, Ailie Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep24957	journal	April 2016
Analysis of the functional compatibility of SIV capsid sequences in the context of the FIV gag precursor Ovejero, César A.; Affranchino, José L.; González, Silvia A. PLOS ONE, Vol. 12, Issue 5 https://doi.org/10.1371/journal.pone.0177297	journal	May 2017
Structure of Drosophila melanogaster ARC1 reveals a repurposed molecule with characteristics of retroviral Gag. Cottee, Matthew A.; Letham, Suzanne C.; Young, George R. The Francis Crick Institute https://doi.org/10.25418/crick.11695380.v1	text	January 2020
Comparison of Biochemical Properties of HIV-1 and HIV-2 Capsid Proteins Miyazaki, Yasuyuki; Miyake, Ariko; Doi, Noya Frontiers in Microbiology, Vol. 8 https://doi.org/10.3389/fmicb.2017.01082	journal	June 2017
Role for Gag-CA Interdomain Linker in Primate Lentiviral Replication Doi, Naoya; Koma, Takaaki; Adachi, Akio Frontiers in Microbiology, Vol. 10 https://doi.org/10.3389/fmicb.2019.01831	journal	August 2019
Properties and Functions of Feline Immunodeficiency Virus Gag Domains in Virion Assembly and Budding González, Silvia; Affranchino, José Viruses, Vol. 10, Issue 5 https://doi.org/10.3390/v10050261	journal	May 2018
HIV Capsid and Integration Targeting Engelman, Alan N. Viruses, Vol. 13, Issue 1 https://doi.org/10.3390/v13010125	journal	January 2021
Visualizing HIV-1 Capsid and Its Interactions with Antivirals and Host Factors Wilbourne, Morganne; Zhang, Peijun Viruses, Vol. 13, Issue 2 https://doi.org/10.3390/v13020246	journal	February 2021
Closing the net on retroviruses Luban, Jeremy eLife, Vol. 5 https://doi.org/10.7554/elife.18243	journal	July 2016

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