Rapid detection of Ebola virus with a reagent-free, point-of-care biosensor

Baca, Justin T.; Severns, Virginia; Lovato, Debbie; Branch, Darren W.; Larson, Richard S.

doi:10.3390/s150408605

Title: Rapid detection of Ebola virus with a reagent-free, point-of-care biosensor

Journal Article · Tue Apr 14 00:00:00 EDT 2015 · Sensors

DOI:https://doi.org/10.3390/s150408605· OSTI ID:1213407

Baca, Justin T. ^[1]; Severns, Virginia ^[1]; Lovato, Debbie ^[1]; Branch, Darren W. ^[2]; Larson, Richard S. ^[1]

Univ. of New Mexico School of Medicine, Albuquerque, NM (United States)
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

Surface acoustic wave (SAW) sensors can rapidly detect Ebola antigens at the point-of-care without the need for added reagents, sample processing, or specialized personnel. This preliminary study demonstrates SAW biosensor detection of the Ebola virus in a concentration-dependent manner. The detection limit with this methodology is below the average level of viremia detected on the first day of symptoms by PCR. We observe a log-linear sensor response for highly fragmented Ebola viral particles, with a detection limit corresponding to 1.9 × 10⁴ PFU/mL prior to virus inactivation. We predict greatly improved sensitivity for intact, infectious Ebola virus. This point-of-care methodology has the potential to detect Ebola viremia prior to symptom onset, greatly enabling infection control and rapid treatment. This biosensor platform is powered by disposable AA batteries and can be rapidly adapted to detect other emerging diseases in austere conditions.

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Research Organization:: Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC04-94AL85000

OSTI ID:: 1213407

Journal Information:: Sensors, Vol. 15, Issue 4; ISSN 1424-8220

Publisher:: MDPI AGCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 35 works

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Micro/nano acoustofluidics: materials, phenomena, design, devices, and applications Connacher, William; Zhang, Naiqing; Huang, An Lab on a Chip, Vol. 18, Issue 14 https://doi.org/10.1039/c8lc00112j	journal	January 2018
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Diagnosis of Ebola Virus Disease: Past, Present, and Future Broadhurst, M. Jana; Brooks, Tim J. G.; Pollock, Nira R. Clinical Microbiology Reviews, Vol. 29, Issue 4 https://doi.org/10.1128/cmr.00003-16	journal	July 2016
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A sensitive and specific point-of-care detection assay for Zaire Ebola virus Zhang, Xiao-Ai; Li, Sabrina; Ching, Jesus Emerging Microbes & Infections, Vol. 6, Issue 1 https://doi.org/10.1038/emi.2016.134	journal	January 2017
Molecular detection and point-of-care testing in Ebola virus disease and other threats: a new global public health framework to stop outbreaks Kost, Gerald J.; Ferguson, William; Truong, Anh-Thu Expert Review of Molecular Diagnostics, Vol. 15, Issue 10 https://doi.org/10.1586/14737159.2015.1079776	journal	September 2015
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60 APPLIED LIFE SCIENCES
pathogens
infectious diseases
viruses
piezoelectric
biosensors
diagnosis
SAW
surface acoustic wave

Title: Rapid detection of Ebola virus with a reagent-free, point-of-care biosensor

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