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Title: Reagent-free and portable detection of Bacillus anthracis spores using a microfluidic incubator and smartphone microscope

Journal Article · · Analyst, 140(18):6269-6276
DOI:https://doi.org/10.1039/C5AN01304F· OSTI ID:1213000

Rapid, cost-effective bacterial detection systems are needed to respond to potential biothreat events. Here we report the use of smartphone-based microscopy in combination with a simple microfluidic incubation device to detect 5000 Bacillus anthracis spores in 3 hours. This field-deployable approach is compatible with real-time PCR for secondary confirmation.

Research Organization:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC05-76RL01830
OSTI ID:
1213000
Report Number(s):
PNNL-SA-109030; 47618
Journal Information:
Analyst, 140(18):6269-6276, Journal Name: Analyst, 140(18):6269-6276
Country of Publication:
United States
Language:
English

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