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Title: Radiation damage in a micron-sized protein crystal studied via reciprocal space mapping and Bragg coherent diffractive imaging

Abstract

For laboratory and synchrotron based X-ray sources, radiation damage has posed a significant barrier to obtaining high-resolution structural data from biological macromolecules. The problem is particularly acute for micron-sized crystals where the weaker signal often necessitates the use of higher intensity beams to obtain the relevant data. Here, we employ a combination of techniques, including Bragg coherent diffractive imaging to characterise the radiation induced damage in a micron-sized protein crystal over time. The approach we adopt here could help screen for potential protein crystal candidates for measurement at X-ray free election laser sources.

Authors:
 [1];  [2];  [1];  [3];  [4];  [5];  [5];  [6]
  1. La Trobe Univ., Melbourne (Australia); CSIRO Manufacturing Flagship, Parkville (Australia)
  2. La Trobe Univ., Melbourne (Australia)
  3. Univ. of Oxford, Oxford (United Kingdom)
  4. SLAC National Accelerator Lab., Menlo Park, CA (United States); Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
  5. Argonne National Lab. (ANL), Argonne, IL (United States)
  6. La Trobe Univ., Melbourne (Australia); Melbourne Centre for Nanofabrication, Melbourne (Australia)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1212299
Alternate Identifier(s):
OSTI ID: 1421048
Grant/Contract Number:  
AC02-06CH11357
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Structural Dynamics
Additional Journal Information:
Journal Volume: 2; Journal Issue: 4; Journal ID: ISSN 2329-7778
Publisher:
American Crystallographic Association/AIP
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; proteins; radiation damage; x-ray diffraction; crystal structure; reciprocal space

Citation Formats

Coughlan, H. D., Darmanin, C., Phillips, N. W., Hofmann, F., Clark, J. N., Harder, R. J., Vine, D. J., and Abbey, B. Radiation damage in a micron-sized protein crystal studied via reciprocal space mapping and Bragg coherent diffractive imaging. United States: N. p., 2015. Web. doi:10.1063/1.4919641.
Coughlan, H. D., Darmanin, C., Phillips, N. W., Hofmann, F., Clark, J. N., Harder, R. J., Vine, D. J., & Abbey, B. Radiation damage in a micron-sized protein crystal studied via reciprocal space mapping and Bragg coherent diffractive imaging. United States. https://doi.org/10.1063/1.4919641
Coughlan, H. D., Darmanin, C., Phillips, N. W., Hofmann, F., Clark, J. N., Harder, R. J., Vine, D. J., and Abbey, B. Wed . "Radiation damage in a micron-sized protein crystal studied via reciprocal space mapping and Bragg coherent diffractive imaging". United States. https://doi.org/10.1063/1.4919641. https://www.osti.gov/servlets/purl/1212299.
@article{osti_1212299,
title = {Radiation damage in a micron-sized protein crystal studied via reciprocal space mapping and Bragg coherent diffractive imaging},
author = {Coughlan, H. D. and Darmanin, C. and Phillips, N. W. and Hofmann, F. and Clark, J. N. and Harder, R. J. and Vine, D. J. and Abbey, B.},
abstractNote = {For laboratory and synchrotron based X-ray sources, radiation damage has posed a significant barrier to obtaining high-resolution structural data from biological macromolecules. The problem is particularly acute for micron-sized crystals where the weaker signal often necessitates the use of higher intensity beams to obtain the relevant data. Here, we employ a combination of techniques, including Bragg coherent diffractive imaging to characterise the radiation induced damage in a micron-sized protein crystal over time. The approach we adopt here could help screen for potential protein crystal candidates for measurement at X-ray free election laser sources.},
doi = {10.1063/1.4919641},
url = {https://www.osti.gov/biblio/1212299}, journal = {Structural Dynamics},
issn = {2329-7778},
number = 4,
volume = 2,
place = {United States},
year = {2015},
month = {4}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 7 works
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    Works referencing / citing this record:

    Nanoscale imaging of the full strain tensor of specific dislocations extracted from a bulk sample
    journal, January 2020


    Bragg coherent diffraction imaging and metrics for radiation damage in protein micro-crystallography
    journal, January 2017


    Resolution and dose dependence of radiation damage in biomolecular systems
    journal, September 2019