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Title: Insights into the respiratory tract microbiota of patients with cystic fibrosis during early Pseudomonas aeruginosa colonization

Journal Article · · SpringerPlus
 [1];  [1];  [2];  [3];  [4];  [1];  [5];  [1];  [6];  [1];  [1];  [3]
  1. Univ. of Brest, Brest Cedex (France)
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Research and Development Luminy Biotech Enterprises, Marseille (France)
  3. Univ. of Brest, Brest Cedex (France); Univ. Hospital of Brest, Brest (France)
  4. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  5. Univ. Hospital of Brest, Brest (France)
  6. CF Center, Roscoff (France)
+ Show Author Affiliations

Pseudomonas aeruginosa plays a major role in cystic fibrosis (CF) progression. Therefore, it is important to understand the initial steps of P. aeruginosa infection. The structure and dynamics of CF respiratory tract microbial communities during the early stages of P. aeruginosa colonization were characterized by pyrosequencing and cloning-sequencing. The respiratory microbiota showed high diversity, related to the young age of the CF cohort (mean age 10 years). Wide inter- and intra-individual variations were revealed. A common core microbiota of 5 phyla and 13 predominant genera was found, the majority of which were obligate anaerobes. A few genera were significantly more prevalent in patients never infected by P. aeruginosa. Persistence of an anaerobic core microbiota regardless of P. aeruginosa status suggests a major role of certain anaerobes in the pathophysiology of lung infections in CF. Some genera may be potential biomarkers of pulmonary infection state.

Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Sponsoring Organization:
USDOE; French Cystic Fibrosis Association: 'Vaincre la Mucoviscidose'; French Ministry of Higher Education and Research (Ministère de l’Enseignement Supérieur et de la Recherche: MESR, France)
Grant/Contract Number:
AC05-76RL01830
OSTI ID:
1212084
Journal Information:
SpringerPlus, Vol. 4, Issue 1; ISSN 2193-1801
Publisher:
SpringerCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 22 works
Citation information provided by
Web of Science

References (40)

Sequencing ancient calcified dental plaque shows changes in oral microbiota with dietary shifts of the Neolithic and Industrial revolutions journal February 2013
Role of respiratory viruses in pulmonary exacerbations in children with cystic fibrosis journal September 2012
Impact of the CFTR-Potentiator Ivacaftor on Airway Microbiota in Cystic Fibrosis Patients Carrying A G551D Mutation journal April 2015
Quantitative Analysis of the Human Airway Microbial Ecology Reveals a Pervasive Signature for Cystic Fibrosis journal September 2012
Comparison of Microbiomes from Different Niches of Upper and Lower Airways in Children and Adolescents with Cystic Fibrosis journal January 2015
QIIME allows analysis of high-throughput community sequencing data journal April 2010
Changes in Cystic Fibrosis Airway Microbiota at Pulmonary Exacerbation journal June 2013
Sputum Microbiota in Tuberculosis as Revealed by 16S rRNA Pyrosequencing journal January 2013
Airway Microbiota and Pathogen Abundance in Age-Stratified Cystic Fibrosis Patients journal June 2010
UPARSE: highly accurate OTU sequences from microbial amplicon reads journal August 2013
UCHIME improves sensitivity and speed of chimera detection journal June 2011
Airway microbiota and bronchial hyperresponsiveness in patients with suboptimally controlled asthma journal February 2011
Relationship between cystic fibrosis respiratory tract bacterial communities and age, genotype, antibiotics and Pseudomonas aeruginosa: Ecology of the cystic fibrosis respiratory tract bacterial community journal February 2010
Acceleration of lung disease in children with cystic fibrosis after Pseudomonas aeruginosa acquisition journal October 2001
Proposal of a quantitative PCR-based protocol for an optimal Pseudomonas aeruginosa detection in patients with cystic fibrosis journal January 2013
Interactive Tree Of Life (iTOL): an online tool for phylogenetic tree display and annotation journal October 2006
Assessment of the microbial diversity at the surface of Livarot cheese using culture-dependent and independent approaches journal July 2009
Clinical outcome after early Pseudomonas aeruginosa infection in cystic fibrosis journal May 2001
Design of 16S rRNA gene primers for 454 pyrosequencing of the human foregut microbiome journal January 2010
Reduced Bacterial Colony Count of Anaerobic Bacteria Is Associated with a Worsening in Lung Clearance Index and Inflammation in Cystic Fibrosis journal May 2015
FastTree: Computing Large Minimum Evolution Trees with Profiles instead of a Distance Matrix journal April 2009
Upper aero-digestive contamination by Pseudomonas aeruginosa and implications in Cystic Fibrosis journal January 2015
Predominant pathogen competition and core microbiota divergence in chronic airway infection journal July 2014
The polymicrobial nature of airway infections in cystic fibrosis: Cangene Gold Medal LectureThis article is based on a presentation by Dr. Christopher Sibley at the 60th Annual Meeting of the Canadian Society of Microbiologists in Hamilton, Ontario, on 15 June 2010. Dr. Sibley was the recipient of the 2010 Cangene Gold Medal as the Canadian Graduate Student Microbiologist of the Year, an annual award sponsored by Cangene Corporation intended to recognize excellence in graduate research. journal February 2011
Cystic fibrosis: a polymicrobial infectious disease journal June 2006
Discerning the Complexity of Community Interactions Using a Drosophila Model of Polymicrobial Infections journal October 2008
Investigating Deep Phylogenetic Relationships among Cyanobacteria and Plastids by Small Subunit rRNA Sequence Analysis journal July 1999
Prevention of chronic Pseudomonas aeruginosa colonisation in cystic fibrosis by early treatment journal September 1991
Partitioning core and satellite taxa from within cystic fibrosis lung bacterial communities journal December 2010
Application of a Neutral Community Model To Assess Structuring of the Human Lung Microbiome journal January 2015
The role of respiratory viruses in cystic fibrosis journal July 2008
Spatial distribution of microbial communities in the cystic fibrosis lung journal July 2011
Inflammation and Airway Microbiota during Cystic Fibrosis Pulmonary Exacerbations journal April 2013
Microbial community structure and functional properties in permanently and seasonally flooded areas in Poyang Lake journal March 2020
Succession of endophytic fungi and arbuscular mycorrhizal fungi associated with the growth of plant and their correlation with secondary metabolites in the roots of plants journal April 2021
Detection of anaerobic bacteria in high numbers in sputum from patients with cystic fibrosis text January 2008
Evaluation of a new definition for chronic Pseudomonas aeruginosa infection in cystic fibrosis patients journal March 2003
Infant gut microbiota characteristics generally do not modify effects of lipid-based nutrient supplementation on growth or inflammation: secondary analysis of a randomized controlled trial in Malawi journal September 2020
Anaerobic Bacterial Infection in Patients with Cystic Fibrosis journal November 2008
Detection of Anaerobic Bacteria in High Numbers in Sputum from Patients with Cystic Fibrosis journal May 2008

Cited By (10)

The airway microbiota in early cystic fibrosis lung disease journal August 2017
Improving outcomes of infections in cystic fibrosis in the era of CFTR modulator therapy journal November 2019
Inhibitory role of acyl homoserine lactones in hemolytic activity and viability of Streptococcus pyogenes M6 S165 journal March 2017
Viruses in cystic fibrosis patients’ airways journal March 2017
Anaerobes in cystic fibrosis patients’ airways journal January 2019
The diagnosis and management of respiratory viral infections in cystic fibrosis journal February 2017
Cystic Fibrosis Airway Microbiome: Overturning the Old, Opening the Way for the New journal October 2017
Cystic fibrosis lung environment and Pseudomonas aeruginosa infection journal December 2016
Characterization of antibiotic resistance and host-microbiome interactions in the human upper respiratory tract during influenza infection journal March 2020
Strong incidence of Pseudomonas aeruginosa on bacterial rrs and ITS genetic structures of cystic fibrosis sputa journal March 2017

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59 BASIC BIOLOGICAL SCIENCES
cystic fibrosis
respiratory tract microbiota
Pseudomonas aeruginosa
early colonization
anaerobes
respiratory viruses