Heterologous production of polyketides by modular type I polyketide synthases in Escherichia coli

Journal Article · Mon Oct 01 00:00:00 EDT 2012 · CURRENT OPINION IN BIOTECHNOLOGY

DOI:https://doi.org/10.1016/j.copbio.2011.12.029· OSTI ID:1211026

Yuzawa, S; Kim, W; Katz, L; Keasling, JD

Heterologous production of polyketide compounds, an important class of natural products with complex chemical structures, was first demonstrated with Streptomyces parvulus in 1984. Although Streptomyces strains are good first options for heterologous polyketide biosynthesis, their slow growth kinetics prompt other hosts to also be considered. Escherichia coli provides key elements of an ideal host in terms of the growth rate, culture conditions, and available recombinant DNA tools. Here we review the current status and potential for metabolic engineering of polyketides in E. coli.

• Sponsoring Organization: USDOE Advanced Research Projects Agency - Energy (ARPA-E)
• DOE Contract Number: DE-AR0000091
• OSTI ID: 1211026
• Journal Information: CURRENT OPINION IN BIOTECHNOLOGY, Vol. 23, Issue 5; ISSN 0958-1669
• Country of Publication: United States
• Language: English