Genome-scale modeling for metabolic engineering

Journal Article · Tue Jan 13 00:00:00 EST 2015 · Journal of Industrial Microbiology and Biotechnology

DOI:https://doi.org/10.1007/s10295-014-1576-3· OSTI ID:1210989

Simeonidis, E; Price, ND

We focus on the application of constraint-based methodologies and, more specifically, flux balance analysis in the field of metabolic engineering, and enumerate recent developments and successes of the field. We also review computational frameworks that have been developed with the express purpose of automatically selecting optimal gene deletions for achieving improved production of a chemical of interest. The application of flux balance analysis methods in rational metabolic engineering requires a metabolic network reconstruction and a corresponding in silico metabolic model for the microorganism in question. For this reason, we additionally present a brief overview of automated reconstruction techniques. Finally, we emphasize the importance of integrating metabolic networks with regulatory information-an area which we expect will become increasingly important for metabolic engineering-and present recent developments in the field of metabolic and regulatory integration.

• Sponsoring Organization: USDOE Advanced Research Projects Agency - Energy (ARPA-E)
• DOE Contract Number: DE-AR0000426
• OSTI ID: 1210989
• Journal Information: Journal of Industrial Microbiology and Biotechnology, Vol. 42, Issue 3; ISSN 1367-5435
• Country of Publication: United States
• Language: English