Oxygen-­dependent regulation of bacterial lipid production

Lemmer, Kimberly C.; Dohnalkova, Alice C.; Noguera, Daniel R.; Donohue, Timothy J.

doi:10.1128/JB.02510-14

Title: Oxygen-dependent regulation of bacterial lipid production

Journal Article · Sat May 02 00:00:00 EDT 2015 · Journal of Bacteriology

DOI:https://doi.org/10.1128/JB.02510-14· OSTI ID:1208744

Lemmer, Kimberly C. ^[1]; Dohnalkova, Alice C. ^[2]; Noguera, Daniel R. ^[1]; Donohue, Timothy J. ^[1]

Univ. of Wisconsin, Madison, WI (United States)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Understanding the mechanisms of lipid accumulation in microorganisms is important for several reasons. In addition to providing insight into assembly of biological membranes, lipid accumulation has important applications in the production of renewable fuels and chemicals. The photosynthetic bacterium Rhodobacter sphaeroides is an attractive organism to study lipid accumulation, as it has the somewhat unique ability to increase membrane production at low O₂ tensions. Under these conditions, R. sphaeroides develops invaginations of the cytoplasmic membrane to increase its membrane surface area for housing of the membrane-bound components of its photosynthetic apparatus. Here we use fatty acid levels as a reporter of membrane lipid content. We show that, under low-O₂ and anaerobic conditions, the total fatty acid content per cell increases 3-fold. We also find that the increases in the amount of fatty acid and photosynthetic pigment per cell are correlated as O₂ tensions or light intensity are changed. To ask if lipid and pigment accumulation were genetically separable, we analyzed strains with mutations in known photosynthetic regulatory pathways. While a strain lacking AppA failed to induce photosynthetic pigment-protein complex accumulation, it increased fatty acid content under low O2 conditions. We also found that an intact PrrBA pathway is required for low O2-induced fatty acid accumulation. In conclusion, our findings suggest a previously unknown role of R. sphaeroides transcriptional regulators in increasing fatty acid and phospholipid accumulation in response to decreased O₂ tension.

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Research Organization:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-76RL01830; FC02-07ER64494

OSTI ID:: 1208744

Report Number(s):: PNNL-SA-106866; 48544; 48815; KP1704020

Journal Information:: Journal of Bacteriology, Vol. 197, Issue 9; ISSN 0021-9193

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 9 works

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