Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses

Schostag, Morten; Stibal, Marek; Jacobsen, Carsten S.; Bælum, Jacob; Taş, Neslihan; Elberling, Bo; Jansson, Janet K.; Semenchuk, Philipp; Priemé, Anders

doi:10.3389/fmicb.2015.00399

Title: Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses

Journal Article · Thu Apr 30 00:00:00 EDT 2015 · Frontiers in Microbiology

DOI:https://doi.org/10.3389/fmicb.2015.00399· OSTI ID:1204352

Schostag, Morten ^[1]; Stibal, Marek ^[1]; Jacobsen, Carsten S. ^[2]; Bælum, Jacob ^[3]; Taş, Neslihan ^[4]; Elberling, Bo ^[1]; Jansson, Janet K. ^[5]; Semenchuk, Philipp ^[1]; Priemé, Anders ^[1]

Univ. of Copenhagen (Denmark)
Aarhus Univ. (Denmark)
Technical Univ. of Denmark, Lyngby (Denmark)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

The active layer of soil overlaying permafrost in the Arctic is subjected to dramatic annual changes in temperature and soil chemistry, which likely affect bacterial activity and community structure. We studied seasonal variations in the bacterial community of active layer soil from Svalbard (78°N) by co-extracting DNA and RNA from 12 soil cores collected monthly over a year. PCR amplicons of 16S rRNA genes (DNA) and reverse transcribed transcripts (cDNA) were quantified and sequenced to test for the effect of low winter temperature and seasonal variation in concentration of easily degradable organic matter on the bacterial communities. The copy number of 16S rRNA genes and transcripts revealed no distinct seasonal changes indicating potential bacterial activity during winter despite soil temperatures well below -10°C. Multivariate statistical analysis of the bacterial diversity data (DNA and cDNA libraries) revealed a season-based clustering of the samples, and, e.g., the relative abundance of potentially active Cyanobacteria peaked in June and Alphaproteobacteria increased over the summer and then declined from October to November. The structure of the bulk (DNA-based) community was significantly correlated with pH and dissolved organic carbon, while the potentially active (RNA-based) community structure was not significantly correlated with any of the measured soil parameters. A large fraction of the 16S rRNA transcripts was assigned to nitrogen-fixing bacteria (up to 24% in June) and phototrophic organisms (up to 48% in June) illustrating the potential importance of nitrogen fixation in otherwise nitrogen poor Arctic ecosystems and of phototrophic bacterial activity on the soil surface.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE; Danish National Research Foundation

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1204352

Journal Information:: Frontiers in Microbiology, Vol. 6; ISSN 1664-302X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 62 works

Citation information provided by
Web of Science

References (65)

Global analysis of nitrogen and phosphorus limitation Ecosystems, NCEAS 10022: Shurin: Comparing Trophic Structure Across; (Extended), NCEAS 11981: Shurin: Comparing Trophic Structure Across Ecosystems; Synthesis, National Center For Ecological Analysis And KNB Data Repository https://doi.org/10.5063/aa/nceas.347.3	dataset	January 2007
Effect of Freeze-Thaw Cycles on Bacterial Communities of Arctic Tundra Soil Männistö, Minna K.; Tiirola, Marja; Häggblom, Max M. Microbial Ecology, Vol. 58, Issue 3 https://doi.org/10.1007/s00248-009-9516-x	journal	April 2009
Deepened snow alters soil microbial nutrient limitations in arctic birch hummock tundra Buckeridge, Kate M.; Grogan, Paul Applied Soil Ecology, Vol. 39, Issue 2 https://doi.org/10.1016/j.apsoil.2007.12.010	journal	June 2008
Seasonal dynamics of nutrients and bacterial communities in unvegetated alpine glacier forefields Lazzaro, A.; Brankatschk, R.; Zeyer, J. Applied Soil Ecology, Vol. 53 https://doi.org/10.1016/j.apsoil.2011.10.013	journal	February 2012
A surface-mediated origin of the RNA world: biogenic activities of clay-adsorbed RNA molecules Franchi, Marco; Gallori, Enzo Gene, Vol. 346 https://doi.org/10.1016/j.gene.2004.11.002	journal	February 2005
Late snowmelt delays plant development and results in lower reproductive success in the High Arctic Cooper, Elisabeth J.; Dullinger, Stefan; Semenchuk, Philipp Plant Science, Vol. 180, Issue 1 https://doi.org/10.1016/j.plantsci.2010.09.005	journal	January 2011
Bacterial communities in tetrachloroethene-polluted groundwaters: A case study Kotik, Michael; Davidová, Anna; Voříšková, Jana Science of The Total Environment, Vol. 454-455 https://doi.org/10.1016/j.scitotenv.2013.02.082	journal	June 2013
A cross-seasonal comparison of active and total bacterial community composition in Arctic tundra soil using bromodeoxyuridine labeling McMahon, Shawna K.; Wallenstein, Matthew D.; Schimel, Joshua P. Soil Biology and Biochemistry, Vol. 43, Issue 2 https://doi.org/10.1016/j.soilbio.2010.10.013	journal	February 2011
The seasonal pattern of soil microbial community structure in mesic low arctic tundra Buckeridge, Kate M.; Banerjee, Samiran; Siciliano, Steven D. Soil Biology and Biochemistry, Vol. 65 https://doi.org/10.1016/j.soilbio.2013.06.012	journal	October 2013
Soil fertility is associated with fungal and bacterial richness, whereas pH is associated with community composition in polar soil microbial communities Siciliano, Steven D.; Palmer, Anne S.; Winsley, Tristrom Soil Biology and Biochemistry, Vol. 78 https://doi.org/10.1016/j.soilbio.2014.07.005	journal	November 2014
Temperature-dependent changes in the soil bacterial community in limed and unlimed soil Pettersson, Marie; BÃ¥Ã¥th, Erland FEMS Microbiology Ecology, Vol. 45, Issue 1 https://doi.org/10.1016/s0168-6496(03)00106-5	journal	July 2003
Biogeochemistry of Metalliferous Peats: Sulfur Speciation and Depth Distributions of dsr AB Genes and Cd, Fe, Mn, S, and Zn in Soil Cores Martínez, Carmen Enid; Yáñez, Carolina; Yoon, Soh-joung Environmental Science & Technology, Vol. 41, Issue 15 https://doi.org/10.1021/es070555v	journal	August 2007
Pyrosequencing enumerates and contrasts soil microbial diversity Roesch, Luiz F. W.; Fulthorpe, Roberta R.; Riva, Alberto The ISME Journal, Vol. 1, Issue 4 https://doi.org/10.1038/ismej.2007.53	journal	July 2007
A comprehensive survey of soil acidobacterial diversity using pyrosequencing and clone library analyses Jones, Ryan T.; Robeson, Michael S.; Lauber, Christian L. The ISME Journal, Vol. 3, Issue 4 https://doi.org/10.1038/ismej.2008.127	journal	January 2009
Despite strong seasonal responses, soil microbial consortia are more resilient to long-term changes in rainfall than overlying grassland Cruz-Martínez, Karelyn; Suttle, K. Blake; Brodie, Eoin L. The ISME Journal, Vol. 3, Issue 6 https://doi.org/10.1038/ismej.2009.16	journal	March 2009
Seasonality and resource availability control bacterial and archaeal communities in soils of a temperate beech forest Rasche, Frank; Knapp, Daniela; Kaiser, Christina The ISME Journal, Vol. 5, Issue 3 https://doi.org/10.1038/ismej.2010.138	journal	September 2010
The functional potential of high Arctic permafrost revealed by metagenomic sequencing, qPCR and microarray analyses Yergeau, Etienne; Hogues, Hervé; Whyte, Lyle G. The ISME Journal, Vol. 4, Issue 9 https://doi.org/10.1038/ismej.2010.41	journal	April 2010
Selection of primers for optimal taxonomic classification of environmental 16S rRNA gene sequences Soergel, David A. W.; Dey, Neelendu; Knight, Rob The ISME Journal, Vol. 6, Issue 7 https://doi.org/10.1038/ismej.2011.208	journal	January 2012
Active and total microbial communities in forest soil are largely different and highly stratified during decomposition Baldrian, Petr; Kolařík, Miroslav; Štursová, Martina The ISME Journal, Vol. 6, Issue 2 https://doi.org/10.1038/ismej.2011.95	journal	July 2011
Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms Caporaso, J. Gregory; Lauber, Christian L.; Walters, William A. The ISME Journal, Vol. 6, Issue 8 https://doi.org/10.1038/ismej.2012.8	journal	March 2012
Organic carbon transformations in high-Arctic peat soils: key functions and microorganisms Tveit, Alexander; Schwacke, Rainer; Svenning, Mette M. The ISME Journal, Vol. 7, Issue 2 https://doi.org/10.1038/ismej.2012.99	journal	September 2012
Evaluating rRNA as an indicator of microbial activity in environmental communities: limitations and uses Blazewicz, Steven J.; Barnard, Romain L.; Daly, Rebecca A. The ISME Journal, Vol. 7, Issue 11 https://doi.org/10.1038/ismej.2013.102	journal	July 2013
Bacterial genome replication at subzero temperatures in permafrost Tuorto, Steven J.; Darias, Phillip; McGuinness, Lora R. The ISME Journal, Vol. 8, Issue 1 https://doi.org/10.1038/ismej.2013.140	journal	August 2013
Distinct microbial communities associated with buried soils in the Siberian tundra Gittel, Antje; Bárta, Jiří; Kohoutová, Iva The ISME Journal, Vol. 8, Issue 4 https://doi.org/10.1038/ismej.2013.219	journal	December 2013
Bacterial growth at −15 °C; molecular insights from the permafrost bacterium Planococcus halocryophilus Or1 Mykytczuk, Nadia C. S.; Foote, Simon J.; Omelon, Chris R. The ISME Journal, Vol. 7, Issue 6 https://doi.org/10.1038/ismej.2013.8	journal	February 2013
The effect of permafrost thaw on old carbon release and net carbon exchange from tundra Schuur, Edward A. G.; Vogel, Jason G.; Crummer, Kathryn G. Nature, Vol. 459, Issue 7246 https://doi.org/10.1038/nature08031	journal	May 2009
Metagenomic analysis of a permafrost microbial community reveals a rapid response to thaw Mackelprang, Rachel; Waldrop, Mark P.; DeAngelis, Kristen M. Nature, Vol. 480, Issue 7377 https://doi.org/10.1038/nature10576	journal	November 2011
Past extreme warming events linked to massive carbon release from thawing permafrost DeConto, Robert M.; Galeotti, Simone; Pagani, Mark Nature, Vol. 484, Issue 7392 https://doi.org/10.1038/nature10929	journal	April 2012
Multi-omics of permafrost, active layer and thermokarst bog soil microbiomes Hultman, Jenni; Waldrop, Mark P.; Mackelprang, Rachel Nature, Vol. 521, Issue 7551 https://doi.org/10.1038/nature14238	journal	March 2015
Plot-scale evidence of tundra vegetation change and links to recent summer warming Elmendorf, Sarah C.; Henry, Gregory H. R.; Hollister, Robert D. Nature Climate Change, Vol. 2, Issue 6 https://doi.org/10.1038/nclimate1465	journal	April 2012
Long-term CO2 production following permafrost thaw Elberling, Bo; Michelsen, Anders; Schädel, Christina Nature Climate Change, Vol. 3, Issue 10 https://doi.org/10.1038/nclimate1955	journal	July 2013
Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing Bokulich, Nicholas A.; Subramanian, Sathish; Faith, Jeremiah J. Nature Methods, Vol. 10, Issue 1 https://doi.org/10.1038/nmeth.2276	journal	December 2012
UPARSE: highly accurate OTU sequences from microbial amplicon reads Edgar, Robert C. Nature Methods, Vol. 10, Issue 10 https://doi.org/10.1038/nmeth.2604	journal	August 2013
Infant gut microbiota characteristics generally do not modify effects of lipid-based nutrient supplementation on growth or inflammation: secondary analysis of a randomized controlled trial in Malawi Hughes, Riley L.; Arnold, Charles D.; Young, Rebecca R. Scientific Reports, Vol. 10, Issue 1 https://doi.org/10.1038/s41598-020-71922-x	journal	September 2020
Ancient bacteria show evidence of DNA repair Johnson, S. S.; Hebsgaard, M. B.; Christensen, T. R. Proceedings of the National Academy of Sciences, Vol. 104, Issue 36 https://doi.org/10.1073/pnas.0706787104	journal	August 2007
Statistics and characteristics of permafrost and ground-ice distribution in the Northern Hemisphere Zhang, T.; Barry, R. G.; Knowles, K. Polar Geography, Vol. 31, Issue 1-2 https://doi.org/10.1080/10889370802175895	journal	March 2008
UCHIME improves sensitivity and speed of chimera detection Edgar, Robert C.; Haas, Brian J.; Clemente, Jose C. Bioinformatics, Vol. 27, Issue 16 https://doi.org/10.1093/bioinformatics/btr381	journal	June 2011
Design and Experimental Application of a Novel Non-Degenerate Universal Primer Set that Amplifies Prokaryotic 16S rRNA Genes with a Low Possibility to Amplify Eukaryotic rRNA Genes Mori, H.; Maruyama, F.; Kato, H. DNA Research, Vol. 21, Issue 2 https://doi.org/10.1093/dnares/dst052	journal	November 2013
Rhodoplanes gen. nov., a New Genus of Phototrophic Bacteria Including Rhodopseudomonas rosea as Rhodoplanes roseus comb. nov. and Rhodoplanes elegans sp. nov. Hiraishi, A.; Ueda, Y. International Journal of Systematic Bacteriology, Vol. 44, Issue 4 https://doi.org/10.1099/00207713-44-4-665	journal	October 1994
Rhodoplanes serenus sp. nov., a purple non-sulfur bacterium isolated from pond water Okamura, K.; Kanbe, T.; Hiraishi, A. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 59, Issue 3 https://doi.org/10.1099/ijs.0.000174-0	journal	March 2009
Rhodoplanes pokkaliisoli sp. nov., a phototrophic alphaproteobacterium isolated from a waterlogged brackish paddy soil Lakshmi, K. V. N. S.; Sasikala, Ch.; Ramana, Ch. V. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 59, Issue 9 https://doi.org/10.1099/ijs.0.008185-0	journal	July 2009
Granulicella paludicola gen. nov., sp. nov., Granulicella pectinivorans sp. nov., Granulicella aggregans sp. nov. and Granulicella rosea sp. nov., acidophilic, polymer-degrading acidobacteria from Sphagnum peat bogs Pankratov, T. A.; Dedysh, S. N. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 60, Issue 12 https://doi.org/10.1099/ijs.0.021824-0	journal	January 2010
Rhodoplanes piscinae sp. nov. isolated from pond water Chakravarthy, S. Kalyana; Ramaprasad, E. V. V.; Shobha, E. International Journal of Systematic and Evolutionary Microbiology, Vol. 62, Issue Pt_12 https://doi.org/10.1099/ijs.0.037663-0	journal	December 2012
Rhodoplanes oryzae sp. nov., a phototrophic alphaproteobacterium isolated from the rhizosphere soil of paddy Srinivas, A.; Sasikala, Ch.; Ramana, Ch. V. International Journal of Systematic and Evolutionary Microbiology, Vol. 64, Issue Pt_7 https://doi.org/10.1099/ijs.0.063347-0	journal	July 2014
Acidobacteria dominate the active bacterial communities of Arctic tundra with widely divergent winter-time snow accumulation and soil temperatures Männistö, Minna K.; Kurhela, Emilia; Tiirola, Marja FEMS Microbiology Ecology, Vol. 84, Issue 1 https://doi.org/10.1111/1574-6941.12035	journal	November 2012
Bacterial community structure and soil properties of a subarctic tundra soil in Council, Alaska Kim, Hye Min; Jung, Ji Young; Yergeau, Etienne FEMS Microbiology Ecology, Vol. 89, Issue 2 https://doi.org/10.1111/1574-6941.12362	journal	August 2014
Bacterial responses to fluctuations and extremes in temperature and brine salinity at the surface of Arctic winter sea ice Ewert, Marcela; Deming, Jody W. FEMS Microbiology Ecology, Vol. 89, Issue 2 https://doi.org/10.1111/1574-6941.12363	journal	June 2014
Predicting long-term carbon mineralization and trace gas production from thawing permafrost of Northeast Siberia Knoblauch, Christian; Beer, Christian; Sosnin, Alexander Global Change Biology, Vol. 19, Issue 4 https://doi.org/10.1111/gcb.12116	journal	January 2013
Water availability controls microbial temperature responses in frozen soil CO2 production: FROZEN SOILS AND UNFROZEN WATER ÃQuist, Mats G.; Sparrman, Tobias; Klemedtsson, Leif Global Change Biology, Vol. 15, Issue 11 https://doi.org/10.1111/j.1365-2486.2009.01898.x	journal	November 2009
Consistent effects of nitrogen amendments on soil microbial communities and processes across biomes Ramirez, Kelly S.; Craine, Joseph M.; Fierer, Noah Global Change Biology, Vol. 18, Issue 6 https://doi.org/10.1111/j.1365-2486.2012.02639.x	journal	February 2012
Global analysis of nitrogen and phosphorus limitation of primary producers in freshwater, marine and terrestrial ecosystems Elser, James J.; Bracken, Matthew E. S.; Cleland, Elsa E. Ecology Letters, Vol. 10, Issue 12 https://doi.org/10.1111/j.1461-0248.2007.01113.x	journal	December 2007
Transcription dynamics of the functional tfdA gene during MCPA herbicide degradation by Cupriavidus necator AEO106 (pRO101) in agricultural soil Nicolaisen, Mette Haubjerg; Bælum, Jacob; Jacobsen, Carsten Suhr Environmental Microbiology, Vol. 10, Issue 3 https://doi.org/10.1111/j.1462-2920.2007.01476.x	journal	March 2008
Bacterial and fungal community structure in Arctic tundra tussock and shrub soils: Community structure in Arctic tundra tussock and shrub soils Wallenstein, Matthew David; McMahon, Shawna; Schimel, Joshua FEMS Microbiology Ecology, Vol. 59, Issue 2 https://doi.org/10.1111/j.1574-6941.2006.00260.x	journal	February 2007
Multivariate analyses in microbial ecology: Multivariate analyses in microbial ecology Ramette, Alban FEMS Microbiology Ecology, Vol. 62, Issue 2 https://doi.org/10.1111/j.1574-6941.2007.00375.x	journal	November 2007
Soil-specific limitations for access and analysis of soil microbial communities by metagenomics: Limitations in soil metagenomics Lombard, Nathalie; Prestat, Emmanuel; van Elsas, Jan Dirk FEMS Microbiology Ecology, Vol. 78, Issue 1 https://doi.org/10.1111/j.1574-6941.2011.01140.x	journal	June 2011
Comparative genomic and physiological analysis provides insights into the role of Acidobacteria in organic carbon utilization in Arctic tundra soils Rawat, Suman R.; Männistö, Minna K.; Bromberg, Yana FEMS Microbiology Ecology, Vol. 82, Issue 2 https://doi.org/10.1111/j.1574-6941.2012.01381.x	journal	April 2012
Effects of season and experimental warming on the bacterial community in a temperate mountain forest soil assessed by 16S rRNA gene pyrosequencing Kuffner, Melanie; Hai, Brigitte; Rattei, Thomas FEMS Microbiology Ecology, Vol. 82, Issue 3 https://doi.org/10.1111/j.1574-6941.2012.01420.x	journal	June 2012
Relationship of Critical Temperature to Macromolecular Synthesis and Growth Yield in Psychrobacter cryopegella Bakermans, Corien; Nealson, Kenneth H. Journal of Bacteriology, Vol. 186, Issue 8 https://doi.org/10.1128/jb.186.8.2340-2345.2004	journal	April 2004
Functional Ecology of Free-Living Nitrogen Fixation: A Contemporary Perspective Reed, Sasha C.; Cleveland, Cory C.; Townsend, Alan R. Annual Review of Ecology, Evolution, and Systematics, Vol. 42, Issue 1 https://doi.org/10.1146/annurev-ecolsys-102710-145034	journal	December 2011
Spatial and Temporal Variation of Archaeal, Bacterial and Fungal Communities in Agricultural Soils Pereira e. Silva, Michele C.; Dias, Armando Cavalcante Franco; van Elsas, Jan Dirk PLoS ONE, Vol. 7, Issue 12 https://doi.org/10.1371/journal.pone.0051554	journal	December 2012
The Effect of Primer Choice and Short Read Sequences on the Outcome of 16S rRNA Gene Based Diversity Studies Ghyselinck, Jonas; Pfeiffer, Stefan; Heylen, Kim PLoS ONE, Vol. 8, Issue 8 https://doi.org/10.1371/journal.pone.0071360	journal	August 2013
Toward an Ecological Classification of soil Bacteria Fierer, Noah; Bradford, Mark A.; Jackson, Robert B. Ecology, Vol. 88, Issue 6 https://doi.org/10.1890/05-1839	journal	June 2007
Consistent effects of nitrogen fertilization on soil bacterial communities in contrasting systems Ramirez, Kelly S.; Lauber, Christian L.; Knight, Rob Ecology, Vol. 91, Issue 12 https://doi.org/10.1890/10-0426.1	journal	December 2010
Soil bacterial community composition altered by increased nutrient availability in Arctic tundra soils Koyama, Akihiro; Wallenstein, Matthew D.; Simpson, Rodney T. Frontiers in Microbiology, Vol. 5 https://doi.org/10.3389/fmicb.2014.00516	journal	October 2014
Multivariate analyses in microbial ecology Ramette, Alban Nicolas Wiley https://doi.org/10.7892/boris.91419	text	January 2007

Cited By (21)

Bacterial communities in ancient permafrost profiles of Svalbard, Arctic Singh, Purnima; Singh, Shiv M.; Singh, Ram N. Journal of Basic Microbiology, Vol. 57, Issue 12 https://doi.org/10.1002/jobm.201700061	journal	September 2017
Linkages between geochemistry and microbiology in a proglacial terrain in the High Arctic Wojcik, Robin; Donhauser, Johanna; Frey, Beat Annals of Glaciology, Vol. 59, Issue 77 https://doi.org/10.1017/aog.2019.1	journal	December 2018
Bacterial and protozoan dynamics upon thawing and freezing of an active layer permafrost soil Schostag, Morten; Priemé, Anders; Jacquiod, Samuel The ISME Journal, Vol. 13, Issue 5 https://doi.org/10.1038/s41396-019-0351-x	journal	January 2019
Microbial diversity in European alpine permafrost and active layers Frey, Beat; Rime, Thomas; Phillips, Marcia FEMS Microbiology Ecology, Vol. 92, Issue 3 https://doi.org/10.1093/femsec/fiw018	journal	January 2016
The subzero microbiome: microbial activity in frozen and thawing soils Nikrad, Mrinalini P.; Kerkhof, Lee J.; Häggblom, Max M. FEMS Microbiology Ecology, Vol. 92, Issue 6 https://doi.org/10.1093/femsec/fiw081	journal	April 2016
Aeolian dispersal of bacteria in southwest Greenland: their sources, abundance, diversity and physiological states Šantl-Temkiv, Tina; Gosewinkel, Ulrich; Starnawski, Piotr FEMS Microbiology Ecology, Vol. 94, Issue 4 https://doi.org/10.1093/femsec/fiy031	journal	February 2018
Disentangling the complexity of permafrost soil by using high resolution profiling of microbial community composition, key functions and respiration rates Müller, Oliver; Bang‐Andreasen, Toke; White, Richard Allen Environmental Microbiology, Vol. 20, Issue 12 https://doi.org/10.1111/1462-2920.14348	journal	July 2018
Emerging from the ice‐fungal communities are diverse and dynamic in earliest soil developmental stages of a receding glacier Dresch, Philipp; Falbesoner, Johannes; Ennemoser, Chiara Environmental Microbiology, Vol. 21, Issue 5 https://doi.org/10.1111/1462-2920.14598	journal	March 2019
Microbial diversity and biogeography in Arctic soils: Microbial diversity and biogeography Malard, Lucie A.; Pearce, David A. Environmental Microbiology Reports, Vol. 10, Issue 6 https://doi.org/10.1111/1758-2229.12680	journal	August 2018
Rare Taxa Exhibit Disproportionate Cell-Level Metabolic Activity in Enriched Anaerobic Digestion Microbial Communities Jia, Yangyang; Leung, Marcus H. Y.; Tong, Xinzhao mSystems, Vol. 4, Issue 1 https://doi.org/10.1128/msystems.00208-18	journal	January 2019
A comparison of methods used to unveil the genetic and metabolic pool in the built environment Gomez-Silvan, Cinta; Leung, Marcus H. Y.; Grue, Katherine A. Microbiome, Vol. 6, Issue 1 https://doi.org/10.1186/s40168-018-0453-0	journal	April 2018
Molecular Evidence for Metabolically Active Bacteria in the Atmosphere Klein, Ann M.; Bohannan, Brendan J. M.; Jaffe, Daniel A. Frontiers in Microbiology, Vol. 7 https://doi.org/10.3389/fmicb.2016.00772	journal	May 2016
Diversity and Composition of Bacterial Community in Soils and Lake Sediments from an Arctic Lake Area Wang, Neng Fei; Zhang, Tao; Yang, Xiao Frontiers in Microbiology, Vol. 7 https://doi.org/10.3389/fmicb.2016.01170	journal	July 2016
Microbial dynamics in a High Arctic glacier forefield: a combined field, laboratory, and modelling approach Bradley, James A.; Arndt, Sandra; Šabacká, Marie Biogeosciences, Vol. 13, Issue 19 https://doi.org/10.5194/bg-13-5677-2016	journal	January 2016
Linkages between geochemistry and microbiology in a proglacial terrain in the High Arctic Wojcik, Robin; Donhauser, Johanna; Frey, Beat Freie Universität Berlin https://doi.org/10.17169/refubium-2750	text	January 2018
The subzero microbiome: Microbial activity in frozen and thawing soils Nikrad, Mrinalini P.; Kerkhof, Lee J.; Häggblom, Max M. Rutgers University https://doi.org/10.7282/t3571f35	text	January 2017
Microbial diversity in European alpine permafrost and active layers Beat, Frey,; Thomas, Rime,; Marcia, Phillips, ETH Zurich https://doi.org/10.3929/ethz-b-000114910	text	January 2016
Influence of glaciation on mechanisms of mineral weathering in two high Arctic catchments Hindshaw, Ruth S.; Heaton, Tim H. E.; Boyd, Eric S. Chemical Geology, Vol. 420 https://doi.org/10.1016/j.chemgeo.2015.11.004	journal	January 2016
Erratum: Potential microbial contamination during sampling of permafrost soil assessed by tracers Bang-Andreasen, Toke; Schostag, Morten; Priemé, Anders Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/srep46006	journal	April 2017
Bacterial Community Structures in Freshwater Polar Environments of Svalbard Ntougias, Spyridon; Polkowska, Żaneta; Nikolaki, Sofia Microbes and Environments, Vol. 31, Issue 4 https://doi.org/10.1264/jsme2.me16074	journal	January 2016
Microbial Communities and Diversities in Mudflat Sediments Analyzed Using a Modified Metatranscriptomic Method Yan, Yong-Wei; Jiang, Qiu-Yue; Wang, Jian-Gong Frontiers in Microbiology, Vol. 9 https://doi.org/10.3389/fmicb.2018.00093	journal	January 2018

Similar Records

Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses

Journal Article · Thu Apr 30 00:00:00 EDT 2015 · Frontiers in Microbiology, 6:Article No. 399 · OSTI ID:1204352

Schostag, Morten; Stibal, Marek; Jacobsen, Carsten S.; +6 more

The Epsomitic Phototrophic Microbial Mat of Hot Lake, Washington. Community Structural Responses to Seasonal Cycling

Journal Article · Wed Nov 13 00:00:00 EST 2013 · Frontiers in Microbiology · OSTI ID:1204352

Lindemann, Stephen R.; Moran, James J.; Stegen, James C.; +11 more

Alpha- and Gammaproteobacterial Methanotrophs Codominate the Active Methane-Oxidizing Communities in an Acidic Boreal Peat Bog

Journal Article · Fri Feb 12 00:00:00 EST 2016 · Applied and Environmental Microbiology · OSTI ID:1204352

Esson, Kaitlin C.; Lin, Xueju; Kumaresan, Deepak; +3 more

Related Subjects

58 GEOSCIENCES
permafrost active layer
seasonal variation
bacterial community structure
16S rRNA gene
Arctic

Title: Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses

Citation Formats

References (65)

Cited By (21)

Similar Records

Related Subjects