Correlative Electron and Fluorescence Microscopy of Magnetotactic Bacteria in Liquid: Toward In Vivo Imaging

Woehl, Taylor J.; Kashyap, Sanjay; Firlar, Emre; Perez-Gonzalez, Teresa; Faivre, Damien; Trubitsyn, Denis; Bazylinski, Dennis A.; Prozorov, Tanya

doi:10.1038/srep06854

Title: Correlative Electron and Fluorescence Microscopy of Magnetotactic Bacteria in Liquid: Toward In Vivo Imaging

Journal Article · Fri Oct 31 00:00:00 EDT 2014 · Scientific Reports

DOI:https://doi.org/10.1038/srep06854· OSTI ID:1203558

Woehl, Taylor J. ^[1]; Kashyap, Sanjay ^[1]; Firlar, Emre ^[1]; Perez-Gonzalez, Teresa ^[2]; Faivre, Damien ^[2]; Trubitsyn, Denis ^[3]; Bazylinski, Dennis A. ^[3]; Prozorov, Tanya ^[1]

Ames Lab., Ames, IA (United States)
Max Planck Society, Garching (Germany). Max Planck Inst. of Colloids and Interfaces
Univ. of Nevada, Las Vegas, NV (United States)

Magnetotactic bacteria biomineralize ordered chains of uniform, membrane-bound magnetite or greigite nanocrystals that exhibit nearly perfect crystal structures and species-specific morphologies. Transmission electron microscopy (TEM) is a critical technique for providing information regarding the organization of cellular and magnetite structures in these microorganisms. However, conventional TEM can only be used to image air-dried or vitrified bacteria removed from their natural environment. Here we present a correlative scanning TEM (STEM) and fluorescence microscopy technique for imaging viable cells of Magnetospirillum magneticum strain AMB-1 in liquid using an in situ fluid cell TEM holder. Fluorescently labeled cells were immobilized on microchip window surfaces and visualized in a fluid cell with STEM, followed by correlative fluorescence imaging to verify their membrane integrity. Notably, the post-STEM fluorescence imaging indicated that the bacterial cell wall membrane did not sustain radiation damage during STEM imaging at low electron dose conditions. We investigated the effects of radiation damage and sample preparation on the bacteria viability and found that approximately 50% of the bacterial membranes remained intact after an hour in the fluid cell, decreasing to ~30% after two hours. These results represent a first step toward in vivo studies of magnetite biomineralization in magnetotactic bacteria.

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Research Organization:: Ames Laboratory (AMES), Ames, IA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-07CH11358

OSTI ID:: 1203558

Journal Information:: Scientific Reports, Vol. 4; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 37 works

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References (59)

Cobalt Ferrite Nanocrystals: Out-Performing Magnetotactic Bacteria Prozorov, Tanya; Palo, Pierre; Wang, Lijun ACS Nano, Vol. 1, Issue 3 https://doi.org/10.1021/nn700194h	journal	October 2007
Biogenic nanoparticles: production, characterization, and application of bacterial magnetosomes Lang, Claus; Schüler, Dirk Journal of Physics: Condensed Matter, Vol. 18, Issue 38 https://doi.org/10.1088/0953-8984/18/38/S19	journal	September 2006
Erratum: Corrigendum: An acidic protein aligns magnetosomes along a filamentous structure in magnetotactic bacteria Scheffel, André; Gruska, Manuela; Faivre, Damien Nature, Vol. 441, Issue 7090 https://doi.org/10.1038/nature04777	journal	May 2006
Electron microscopy of specimens in liquid de Jonge, Niels; Ross, Frances M. Nature Nanotechnology, Vol. 6, Issue 11, p. 695-704 https://doi.org/10.1038/nnano.2011.161	journal	October 2011
Magnetosome vesicles are present before magnetite formation, and MamA is required for their activation Komeili, A.; Vali, H.; Beveridge, T. J. Proceedings of the National Academy of Sciences, Vol. 101, Issue 11 https://doi.org/10.1073/pnas.0400391101	journal	March 2004
Biological Permanent Magnets Frankel, Richard B. Hyperfine Interactions, Vol. 151/152, Issue 1-4 https://doi.org/10.1023/B:HYPE.0000020407.25316.c3	journal	January 2003
A Novel Protein Tightly Bound to Bacterial Magnetic Particles in Magnetospirillum magneticum Strain AMB-1 Arakaki, Atsushi; Webb, John; Matsunaga, Tadashi Journal of Biological Chemistry, Vol. 278, Issue 10 https://doi.org/10.1074/jbc.M211729200	journal	December 2002
Atomic-Scale Imaging and Spectroscopy for In Situ Liquid Scanning Transmission Electron Microscopy Jungjohann, Katherine L.; Evans, James E.; Aguiar, Jeffery A. Microscopy and Microanalysis, Vol. 18, Issue 3 https://doi.org/10.1017/S1431927612000104	journal	May 2012
Imaging of Self-Assembled Structures: Interpretation of TEM and Cryo-TEM Images Friedrich, Heiner; Frederik, Peter M.; de With, Gijsbertus Angewandte Chemie International Edition, Vol. 49, Issue 43 https://doi.org/10.1002/anie.201001493	journal	September 2010
The Oxygen Effect in Bacterial Cells Irradiated with High-Intensity Pulsed Electrons Epp, Edward R.; Weiss, Herbert; Santomasso, Ann Radiation Research, Vol. 34, Issue 2 https://doi.org/10.2307/3572557	journal	May 1968
Magnetite Crystal Orientation in Magnetosome Chains Körnig, André; Winklhofer, Michael; Baumgartner, Jens Advanced Functional Materials, Vol. 24, Issue 25 https://doi.org/10.1002/adfm.201303737	journal	March 2014
Visualizing Gold Nanoparticle Uptake in Live Cells with Liquid Scanning Transmission Electron Microscopy Peckys, Diana B.; de Jonge, Niels Nano Letters, Vol. 11, Issue 4 https://doi.org/10.1021/nl200285r	journal	April 2011
Nanometer-resolution electron microscopy through micrometers-thick water layers de Jonge, Niels; Poirier-Demers, Nicolas; Demers, Hendrix Ultramicroscopy, Vol. 110, Issue 9 https://doi.org/10.1016/j.ultramic.2010.04.001	journal	August 2010
Nanoscale Imaging of Lithium Ion Distribution During In Situ Operation of Battery Electrode and Electrolyte Holtz, Megan E.; Yu, Yingchao; Gunceler, Deniz Nano Letters, Vol. 14, Issue 3 https://doi.org/10.1021/nl404577c	journal	February 2014
Magnetite formation by a magnetic bacterium capable of growing aerobically Matsunaga, Tadashi; Sakaguchi, Toshifumi; Tadakoro, Fumihiko Applied Microbiology and Biotechnology, Vol. 35, Issue 5 https://doi.org/10.1007/BF00169632	journal	August 1991
Visualization of Iron-Binding Micelles in Acidic Recombinant Biomineralization Protein, MamC Kashyap, Sanjay; Woehl, Taylor; Valverde-Tercedor, Carmen Journal of Nanomaterials, Vol. 2014 https://doi.org/10.1155/2014/320124	journal	January 2014
Silica Biomineralisation in Diatoms: The Model Organism Thalassiosira pseudonana Sumper, Manfred; Brunner, Eike ChemBioChem, Vol. 9, Issue 8 https://doi.org/10.1002/cbic.200700764	journal	May 2008
Magnetic properties of bacterial magnetosomes as potential diagnostic and therapeutic tools Hergt, Rudolf; Hiergeist, Robert; Zeisberger, Matthias Journal of Magnetism and Magnetic Materials, Vol. 293, Issue 1 https://doi.org/10.1016/j.jmmm.2005.01.047	journal	May 2005
Protein-Mediated Synthesis of Uniform Superparamagnetic Magnetite Nanocrystals Prozorov, T.; Mallapragada, S. K.; Narasimhan, B. Advanced Functional Materials, Vol. 17, Issue 6, p. 951-957 https://doi.org/10.1002/adfm.200600448	journal	April 2007
Elongated prismatic magnetite crystals in ALH84001 carbonate globules: Thomas-Keprta, Kathie L.; Bazylinski, Dennis A.; Kirschvink, Joseph L. Geochimica et Cosmochimica Acta, Vol. 64, Issue 23 https://doi.org/10.1016/S0016-7037(00)00481-6	journal	December 2000
Direct in Situ Determination of the Mechanisms Controlling Nanoparticle Nucleation and Growth Woehl, Taylor J.; Evans, James E.; Arslan, Ilke ACS Nano, Vol. 6, Issue 10 https://doi.org/10.1021/nn303371y	journal	September 2012
Nucleation of Iron Oxide Nanoparticles Mediated by Mms6 Protein in Situ Kashyap, Sanjay; Woehl, Taylor J.; Liu, Xunpei ACS Nano, Vol. 8, Issue 9 https://doi.org/10.1021/nn502551y	journal	August 2014
Changes of cell growth and magnetosome biomineralization in Magnetospirillum magneticum AMB-1 after ultraviolet-B irradiation Wang, Yinzhao; Lin, Wei; Li, Jinhua Frontiers in Microbiology, Vol. 4 https://doi.org/10.3389/fmicb.2013.00397	journal	January 2013
Magnetosomes: Nanoscale Magnetic Iron Minerals in Bacteria Frankel, Richard B.; Bazylinski, Dennis A. Nanobiotechnology: Concepts, Applications and Perspectives https://doi.org/10.1002/3527602453.ch10	book	January 2004
Oriented attachment explains cobalt ferrite nanoparticle growth in bioinspired syntheses Wolff, Annalena; Hetaba, Walid; Wißbrock, Marco Beilstein Journal of Nanotechnology, Vol. 5 https://doi.org/10.3762/bjnano.5.23	journal	January 2014
Magnetite Biomineralization in Bacteria Baumgartner, Jens; Faivre, Damien Molecular Biomineralization https://doi.org/10.1007/978-3-642-21230-7_1	book	January 2011
Fully Hydrated Yeast Cells Imaged with Electron Microscopy Peckys, Diana B.; Mazur, Peter; Gould, Kathleen L. Biophysical Journal, Vol. 100, Issue 10 https://doi.org/10.1016/j.bpj.2011.03.045	journal	May 2011
Influence of the synthetic polypeptide c25-mms6 on cobalt ferrite nanoparticle formation Wolff, Annalena; Frese, Katrin; Wißbrock, Marco Journal of Nanoparticle Research, Vol. 14, Issue 10 https://doi.org/10.1007/s11051-012-1161-5	journal	September 2012
Loss of the actin-like protein MamK has pleiotropic effects on magnetosome formation and chain assembly in Magnetospirillum gryphiswaldense: MamK function in M. gryphiswaldense Katzmann, Emanuel; Scheffel, André; Gruska, Manuela Molecular Microbiology, Vol. 77, Issue 1 https://doi.org/10.1111/j.1365-2958.2010.07202.x	journal	May 2010
X-ray Energy-Dispersive Spectrometry During In Situ Liquid Cell Studies Using an Analytical Electron Microscope Zaluzec, Nestor J.; Burke, M. Grace; Haigh, Sarah J. Microscopy and Microanalysis, Vol. 20, Issue 2 https://doi.org/10.1017/S1431927614000154	journal	February 2014
Imaging Protein Structure in Water at 2.7 nm Resolution by Transmission Electron Microscopy Mirsaidov, Utkur M.; Zheng, Haimei; Casana, Yosune Biophysical Journal, Vol. 102, Issue 4 https://doi.org/10.1016/j.bpj.2012.01.009	journal	February 2012
Magnetotactic bacteria form magnetite from a phosphate-rich ferric hydroxide via nanometric ferric (oxyhydr)oxide intermediates Baumgartner, J.; Morin, G.; Menguy, N. Proceedings of the National Academy of Sciences, Vol. 110, Issue 37 https://doi.org/10.1073/pnas.1307119110	journal	August 2013
Magnetosome formation in prokaryotes Bazylinski, Dennis A.; Frankel, Richard B. Nature Reviews Microbiology, Vol. 2, Issue 3 https://doi.org/10.1038/nrmicro842	journal	March 2004
Magnetotactic Bacteria and Magnetosomes Faivre, Damien; Schüler, Dirk Chemical Reviews, Vol. 108, Issue 11 https://doi.org/10.1021/cr078258w	journal	November 2008
CHAPTER 4. Advanced Transmission Electron Microscopy to Explore Early Stages of Bio(mimetic)mineralization Dey, Archan; Sommerdijk, Nico A. J. M. Materials Design Inspired by Nature https://doi.org/10.1039/9781849737555-00074	book	January 2013
Examining the chemistry and magnetism of magnetotactic bacterium Candidatus Magnetovibrio blakemorei strain MV-1 using scanning transmission X-ray microscopy Kalirai, Samanbir S.; Lam, Karen P.; Bazylinski, Dennis A. Chemical Geology, Vol. 300-301 https://doi.org/10.1016/j.chemgeo.2012.01.005	journal	March 2012
Preparation and separation properties of LTA membranes by using 3-aminopropyltriethoxysilane as covalent linker Huang, Aisheng; Liang, Fangyi; Steinbach, Frank Journal of Membrane Science, Vol. 350, Issue 1-2 https://doi.org/10.1016/j.memsci.2009.12.029	journal	March 2010
Electron microscopy of whole cells in liquid with nanometer resolution Jonge, N. d.; Peckys, D. B.; Kremers, G. J. Proceedings of the National Academy of Sciences, Vol. 106, Issue 7 https://doi.org/10.1073/pnas.0809567106	journal	January 2009
Correlative microscopy: Bridging the gap between fluorescence light microscopy and cryo-electron tomography Sartori, Anna; Gatz, Rudolf; Beck, Florian Journal of Structural Biology, Vol. 160, Issue 2 https://doi.org/10.1016/j.jsb.2007.07.011	journal	November 2007
Novel magnetic nanomaterials inspired by magnetotactic bacteria: Topical review Prozorov, Tanya; Bazylinski, Dennis A.; Mallapragada, Surya K. Materials Science and Engineering: R: Reports, Vol. 74, Issue 5 https://doi.org/10.1016/j.mser.2013.04.002	journal	May 2013
Controlled Biomineralization by and Applications of Magnetotactic Bacteria Bazylinski, Dennis A.; Schübbe, Sabrina Advances in Applied Microbiology https://doi.org/10.1016/S0065-2164(07)62002-4	book	January 2007
Learning from Diatoms: Nature's Tools for the Production of Nanostructured Silica Sumper, M.; Brunner, E. Advanced Functional Materials, Vol. 16, Issue 1 https://doi.org/10.1002/adfm.200500616	journal	January 2006
Correlative Fluorescence Microscopy and Scanning Transmission Electron Microscopy of Quantum-Dot-Labeled Proteins in Whole Cells in Liquid Dukes, Madeline J.; Peckys, Diana B.; de Jonge, Niels ACS Nano, Vol. 4, Issue 7 https://doi.org/10.1021/nn1010232	journal	June 2010
Visualizing viral assemblies in a nanoscale biosphere Gilmore, Brian L.; Showalter, Shannon P.; Dukes, Madeline J. Lab Chip, Vol. 13, Issue 2 https://doi.org/10.1039/C2LC41008G	journal	January 2013
Specimen damage caused by the beam of the transmission electron microscope, a correlative reconsideration Stenn, K.; Bahr, G. F. Journal of Ultrastructure Research, Vol. 31, Issue 5-6 https://doi.org/10.1016/S0022-5320(70)90167-X	journal	June 1970
Progress in applications of magnetic nanoparticles in biomedicine Pankhurst, Q. A.; Thanh, N. T. K.; Jones, S. K. Journal of Physics D: Applied Physics, Vol. 42, Issue 22 https://doi.org/10.1088/0022-3727/42/22/224001	journal	November 2009
Magnetosomes Are Cell Membrane Invaginations Organized by the Actin-Like Protein MamK Komeili, A. Science, Vol. 311, Issue 5758 https://doi.org/10.1126/science.1123231	journal	January 2006
Growth and magnetosome formation by microaerophilic Magnetospirillum strains in an oxygen-controlled fermentor Heyen, U.; Schüler, D. Applied Microbiology and Biotechnology, Vol. 61, Issue 5-6 https://doi.org/10.1007/s00253-002-1219-x	journal	February 2003
Visualizing macromolecular complexes with in situ liquid scanning transmission electron microscopy Evans, James E.; Jungjohann, Katherine L.; Wong, Peony C. K. Micron, Vol. 43, Issue 11 https://doi.org/10.1016/j.micron.2012.01.018	journal	November 2012
High-Resolution Electron Microscopy and Spectroscopy of Ferritin in Biocompatible Graphene Liquid Cells and Graphene Sandwiches Wang, Canhui; Qiao, Qiao; Shokuhfar, Tolou Advanced Materials, Vol. 26, Issue 21 https://doi.org/10.1002/adma.201306069	journal	February 2014
Nanoscale Imaging of Whole Cells Using a Liquid Enclosure and a Scanning Transmission Electron Microscope Peckys, Diana B.; Veith, Gabriel M.; Joy, David C. PLoS ONE, Vol. 4, Issue 12 https://doi.org/10.1371/journal.pone.0008214	journal	December 2009
Liquid Scanning Transmission Electron Microscopy: Imaging Protein Complexes in their Native Environment in Whole Eukaryotic Cells Peckys, Diana B.; de Jonge, Niels Microscopy and Microanalysis, Vol. 20, Issue 2 https://doi.org/10.1017/S1431927614000099	journal	February 2014
Experimental procedures to mitigate electron beam induced artifacts during in situ fluid imaging of nanomaterials Woehl, Taylor J.; Jungjohann, Katherine L.; Evans, James E. Ultramicroscopy, Vol. 127 https://doi.org/10.1016/j.ultramic.2012.07.018	journal	April 2013
Variation in Resistance of Natural Isolates ofEscherichia coli O157 to High Hydrostatic Pressure, Mild Heat, and Other Stresses Benito, Amparo; Ventoura, Georgia; Casadei, Maria Applied and Environmental Microbiology, Vol. 65, Issue 4 https://doi.org/10.1128/AEM.65.4.1564-1569.1999	journal	April 1999
Magnetosomes in Magnetotactic Bacteria Scheffel, André; Schüler, Dirk Microbiology Monographs https://doi.org/10.1007/7171_024	book	January 2006
Gold Nanoparticle Uptake in Whole Cells in Liquid Examined by Environmental Scanning Electron Microscopy Peckys, Diana B.; de Jonge, Niels Microscopy and Microanalysis, Vol. 20, Issue 1 https://doi.org/10.1017/s1431927613013986	journal	January 2014
Self-aligned wet-cell for hydrated microbiology observation in TEM Huang, Tsu-Wei; Liu, Shih-Yi; Chuang, Yun-Ju Lab Chip, Vol. 12, Issue 2 https://doi.org/10.1039/c1lc20647h	journal	January 2012
Effects of High Pressure on the Viability, Morphology, Lysis, and Cell Wall Hydrolase Activity of Lactococcus lactis subsp. cremoris Malone, A. S.; Shellhammer, T. H.; Courtney, P. D. Applied and Environmental Microbiology, Vol. 68, Issue 9 https://doi.org/10.1128/aem.68.9.4357-4363.2002	journal	September 2002
Nanoscale Imaging of Lithium Ion Distribution During In Situ Operation of Battery Electrode and Electrolyte Holtz, Megan E.; Yu, Yingchao; Gunceler, Deniz arXiv https://doi.org/10.48550/arxiv.1311.6490	text	January 2013

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