Identification of MicroRNAs and transcript targets in Camelina sativa by deep sequencing and computational methods

Poudel, Saroj; Aryal, Niranjan; Lu, Chaofu; Wang, Tai

doi:10.1371/journal.pone.0121542

Title: Identification of MicroRNAs and transcript targets in Camelina sativa by deep sequencing and computational methods

Journal Article · Tue Mar 31 00:00:00 EDT 2015 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0121542· OSTI ID:1202364

Poudel, Saroj ^[1]; Aryal, Niranjan ^[1]; Lu, Chaofu ^[1]; Wang, Tai ^[2]

Montana State Univ., Bozeman, MT (United States)
Chinese Academy of Sciences (CAS), Beijing (China)

Camelina sativa is an annual oilseed crop that is under intensive development for renewable resources of biofuels and industrial oils. MicroRNAs, or miRNAs, are endogenously encoded small RNAs that play key roles in diverse plant biological processes. Here, we conducted deep sequencing on small RNA libraries prepared from camelina leaves, flower buds and two stages of developing seeds corresponding to initial and peak storage products accumulation. Computational analyses identified 207 known miRNAs belonging to 63 families, as well as 5 novel miRNAs. These miRNAs, especially members of the miRNA families, varied greatly in different tissues and developmental stages. The predicted miRNA target genes are involved in a broad range of physiological functions including lipid metabolism. This report is the first step toward elucidating roles of miRNAs in C. sativa and will provide additional tools to improve this oilseed crop for biofuels and biomaterials.

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Research Organization:: Donald Danforth Plant Science Center, St. Louis, MO (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AR0000202

OSTI ID:: 1202364

Journal Information:: PLoS ONE, Vol. 10, Issue 3; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 16 works

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High throughput sequencing of small RNAs reveals dynamic microRNAs expression of lipid metabolism during Camellia oleifera and C. meiocarpa seed natural drying Feng, Jin-Ling; Yang, Zhi-Jian; Chen, Shi-Pin BMC Genomics, Vol. 18, Issue 1 https://doi.org/10.1186/s12864-017-3923-z	journal	July 2017
Gene Expression Patterns in Roots of Camelina sativa With Enhanced Salinity Tolerance Arising From Inoculation of Soil With Plant Growth Promoting Bacteria Producing 1-Aminocyclopropane-1-Carboxylate Deaminase or Expression the Corresponding acdS Gene Heydarian, Zohreh; Gruber, Margaret; Glick, Bernard R. Frontiers in Microbiology, Vol. 9 https://doi.org/10.3389/fmicb.2018.01297	journal	June 2018
High throughput sequencing of small RNAs reveals dynamic microRNAs expression of lipid metabolism during Camellia oleifera and C. meiocarpa seed natural drying Feng, Jin-Ling; Yang, Zhi-Jian; Chen, Shi-Pin BioMed Central https://doi.org/10.14288/1.0349033	text	January 2017

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