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Title: Correction: Calcium specificity signaling mechanisms in abscisic acid signal transduction in Arabidopsis guard cells

Abstract

One central question is how specificity in cellular responses to the eukaryotic second messenger Ca 2+ is achieved. Plant guard cells, that form stomatal pores for gas exchange, provide a powerful system for in depth investigation of Ca 2+-signaling specificity in plants. In intact guard cells, abscisic acid (ABA) enhances (primes) the Ca 2+-sensitivity of downstream signaling events that result in activation of S-type anion channels during stomatal closure, providing a specificity mechanism in Ca 2+-signaling. However, the underlying genetic and biochemical mechanisms remain unknown. Here we show impairment of ABA signal transduction in stomata of calcium-dependent protein kinase quadruple mutant plants. Interestingly, protein phosphatase 2Cs prevent non-specific Ca 2+-signaling. Moreover, we demonstrate an unexpected interdependence of the Ca 2+-dependent and Ca 2+-independent ABA-signaling branches and the in planta requirement of simultaneous phosphorylation at two key phosphorylation sites in SLAC1. We identify novel mechanisms ensuring specificity and robustness within stomatal Ca 2+-signaling on a cellular, genetic, and biochemical level.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1]
  1. Univ. of California, San Diego, CA (United States)
Publication Date:
Research Org.:
Univ. of California, San Diego, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); National Institutes of Health (NIH); National Science Foundation (NSF)
OSTI Identifier:
1196443
Alternate Identifier(s):
OSTI ID: 1198705; OSTI ID: 1411725
Grant/Contract Number:  
FG02-03ER15449; GM060396-ES010337; MCB1414339
Resource Type:
Journal Article: Published Article
Journal Name:
eLife
Additional Journal Information:
Journal Volume: 4; Journal ID: ISSN 2050-084X
Publisher:
eLife Sciences Publications, Ltd.
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Brandt, Benjamin, Munemasa, Shintaro, Wang, Cun, Nguyen, Desiree, Yong, Taiming, Yang, Paul G., Poretsky, Elly, Belknap, Thomas F., Waadt, Rainer, Alemán, Fernando, and Schroeder, Julian I. Correction: Calcium specificity signaling mechanisms in abscisic acid signal transduction in Arabidopsis guard cells. United States: N. p., 2015. Web. doi:10.7554/eLife.03599.
Brandt, Benjamin, Munemasa, Shintaro, Wang, Cun, Nguyen, Desiree, Yong, Taiming, Yang, Paul G., Poretsky, Elly, Belknap, Thomas F., Waadt, Rainer, Alemán, Fernando, & Schroeder, Julian I. Correction: Calcium specificity signaling mechanisms in abscisic acid signal transduction in Arabidopsis guard cells. United States. doi:10.7554/eLife.03599.
Brandt, Benjamin, Munemasa, Shintaro, Wang, Cun, Nguyen, Desiree, Yong, Taiming, Yang, Paul G., Poretsky, Elly, Belknap, Thomas F., Waadt, Rainer, Alemán, Fernando, and Schroeder, Julian I. Mon . "Correction: Calcium specificity signaling mechanisms in abscisic acid signal transduction in Arabidopsis guard cells". United States. doi:10.7554/eLife.03599.
@article{osti_1196443,
title = {Correction: Calcium specificity signaling mechanisms in abscisic acid signal transduction in Arabidopsis guard cells},
author = {Brandt, Benjamin and Munemasa, Shintaro and Wang, Cun and Nguyen, Desiree and Yong, Taiming and Yang, Paul G. and Poretsky, Elly and Belknap, Thomas F. and Waadt, Rainer and Alemán, Fernando and Schroeder, Julian I.},
abstractNote = {One central question is how specificity in cellular responses to the eukaryotic second messenger Ca2+ is achieved. Plant guard cells, that form stomatal pores for gas exchange, provide a powerful system for in depth investigation of Ca2+-signaling specificity in plants. In intact guard cells, abscisic acid (ABA) enhances (primes) the Ca2+-sensitivity of downstream signaling events that result in activation of S-type anion channels during stomatal closure, providing a specificity mechanism in Ca2+-signaling. However, the underlying genetic and biochemical mechanisms remain unknown. Here we show impairment of ABA signal transduction in stomata of calcium-dependent protein kinase quadruple mutant plants. Interestingly, protein phosphatase 2Cs prevent non-specific Ca2+-signaling. Moreover, we demonstrate an unexpected interdependence of the Ca2+-dependent and Ca2+-independent ABA-signaling branches and the in planta requirement of simultaneous phosphorylation at two key phosphorylation sites in SLAC1. We identify novel mechanisms ensuring specificity and robustness within stomatal Ca2+-signaling on a cellular, genetic, and biochemical level.},
doi = {10.7554/eLife.03599},
journal = {eLife},
issn = {2050-084X},
number = ,
volume = 4,
place = {United States},
year = {2015},
month = {7}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.7554/eLife.03599

Citation Metrics:
Cited by: 37 works
Citation information provided by
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Works referenced in this record:

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
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Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
journal, December 1998