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Title: Cellulase variants

Abstract

This invention provides novel variant cellulolytic enzymes having improved activity and/or stability. In certain embodiments the variant cellulotyic enzymes comprise a glycoside hydrolase with or comprising a substitution at one or more positions corresponding to one or more of residues F64, A226, and/or E246 in Thermobifida fusca Cel9A enzyme. In certain embodiments the glycoside hydrolase is a variant of a family 9 glycoside hydrolase. In certain embodiments the glycoside hydrolase is a variant of a theme B family 9 glycoside hydrolase.

Inventors:
; ; ; ;
Publication Date:
Research Org.:
Novozymes, A/S, Bagsvaerd (Denmark)
Sponsoring Org.:
USDOE
OSTI Identifier:
1193384
Patent Number(s):
9,080,162
Application Number:
14/111,321
Assignee:
Novozymes, A/S (Bagsvaerd, DK)
DOE Contract Number:  
SC0002302
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 Apr 11
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS

Citation Formats

Blazej, Robert, Toriello, Nicholas, Emrich, Charles, Cohen, Richard N., and Koppel, Nitzan. Cellulase variants. United States: N. p., 2015. Web.
Blazej, Robert, Toriello, Nicholas, Emrich, Charles, Cohen, Richard N., & Koppel, Nitzan. Cellulase variants. United States.
Blazej, Robert, Toriello, Nicholas, Emrich, Charles, Cohen, Richard N., and Koppel, Nitzan. Tue . "Cellulase variants". United States. https://www.osti.gov/servlets/purl/1193384.
@article{osti_1193384,
title = {Cellulase variants},
author = {Blazej, Robert and Toriello, Nicholas and Emrich, Charles and Cohen, Richard N. and Koppel, Nitzan},
abstractNote = {This invention provides novel variant cellulolytic enzymes having improved activity and/or stability. In certain embodiments the variant cellulotyic enzymes comprise a glycoside hydrolase with or comprising a substitution at one or more positions corresponding to one or more of residues F64, A226, and/or E246 in Thermobifida fusca Cel9A enzyme. In certain embodiments the glycoside hydrolase is a variant of a family 9 glycoside hydrolase. In certain embodiments the glycoside hydrolase is a variant of a theme B family 9 glycoside hydrolase.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {7}
}

Patent:

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Works referenced in this record:

Kinetic Studies of Thermobifida fusca Cel9A Active Site Mutant Enzymes
journal, August 2004

  • Zhou, Weilin; Irwin, Diana C.; Escovar-Kousen, Jose
  • Biochemistry, Vol. 43, Issue 30, p. 9655-9663
  • DOI: 10.1021/bi049394n

Processivity, Substrate Binding, and Mechanism of Cellulose Hydrolysis by Thermobifida fusca Cel9A
journal, March 2007

  • Li, Y.; Irwin, D. C.; Wilson, D. B.
  • Applied and Environmental Microbiology, Vol. 73, Issue 10
  • DOI: 10.1128/AEM.02960-06

Integration of Computer Modeling and Initial Studies of Site-Directed Mutagenesis to Improve Cellulase Activity on Cel9A from <I>Thermobifida fusca </I>
journal, January 2004

  • Escovar-Kousen, José M.; Wilson, David; Irwin, Diana
  • Applied Biochemistry and Biotechnology, Vol. 113, Issue 1-3
  • DOI: 10.1385/ABAB:113:1-3:287