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Title: Transgenic expression of the dicotyledonous pattern recognition receptor EFR in rice leads to ligand-dependent activation of defense responses

Abstract

Plant plasma membrane localized pattern recognition receptors (PRRs) detect extracellular pathogen-associated molecules. PRRs such as Arabidopsis EFR and rice XA21 are taxonomically restricted and are absent from most plant genomes. Here we show that rice plants expressing EFR or the chimeric receptor EFR::XA21, containing the EFR ectodomain and the XA21 intracellular domain, sense both Escherichia coli- and Xanthomonas oryzae pv. oryzae (Xoo)-derived elf18 peptides at sub-nanomolar concentrations. Treatment of EFR and EFR::XA21 rice leaf tissue with elf18 leads to MAP kinase activation, reactive oxygen production and defense gene expression. Although expression of EFR does not lead to robust enhanced resistance to fully virulent Xoo isolates, it does lead to quantitatively enhanced resistance to weakly virulent Xoo isolates. EFR interacts with OsSERK2 and the XA21 binding protein 24 (XB24), two key components of the rice XA21-mediated immune response. Rice-EFR plants silenced for OsSERK2, or overexpressing rice XB24 are compromised in elf18-induced reactive oxygen production and defense gene expression indicating that these proteins are also important for EFR-mediated signaling in transgenic rice. Taken together, our results demonstrate the potential feasibility of enhancing disease resistance in rice and possibly other monocotyledonous crop species by expression of dicotyledonous PRRs. Our results also suggest thatmore » Arabidopsis EFR utilizes at least a subset of the known endogenous rice XA21 signaling components.« less

Authors:
 [1];  [2];  [2];  [3];  [3];  [1];  [1];  [2];  [4];  [5];  [5];  [5];  [5];  [4];  [3];  [1]
  1. Univ. of California, Davis, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  2. Univ. of California, Davis, CA (United States)
  3. Norwich Research Park, Norwich (United Kingdom)
  4. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  5. US Department of Energy Joint Genome Institute, Walnut Creek, California (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Joint BioEnergy Institute, Emeryville, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1191186
Grant/Contract Number:
AC02-05CH11231
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
PLoS Pathogens
Additional Journal Information:
Journal Volume: 11; Journal Issue: 3; Journal ID: ISSN 1553-7374
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Schwessinger, Benjamin, Bahar, Ofir, Thomas, Nicolas, Holton, Nicolas, Nekrasov, Vladimir, Ruan, Deling, Canlas, Patrick E., Daudi, Arsalan, Petzold, Christopher J., Singan, Vasanth R., Kuo, Rita, Chovatia, Mansi, Daum, Christopher, Heazlewood, Joshua L., Zipfel, Cyril, and Ronald, Pamela C.. Transgenic expression of the dicotyledonous pattern recognition receptor EFR in rice leads to ligand-dependent activation of defense responses. United States: N. p., 2015. Web. doi:10.1371/journal.ppat.1004809.
Schwessinger, Benjamin, Bahar, Ofir, Thomas, Nicolas, Holton, Nicolas, Nekrasov, Vladimir, Ruan, Deling, Canlas, Patrick E., Daudi, Arsalan, Petzold, Christopher J., Singan, Vasanth R., Kuo, Rita, Chovatia, Mansi, Daum, Christopher, Heazlewood, Joshua L., Zipfel, Cyril, & Ronald, Pamela C.. Transgenic expression of the dicotyledonous pattern recognition receptor EFR in rice leads to ligand-dependent activation of defense responses. United States. doi:10.1371/journal.ppat.1004809.
Schwessinger, Benjamin, Bahar, Ofir, Thomas, Nicolas, Holton, Nicolas, Nekrasov, Vladimir, Ruan, Deling, Canlas, Patrick E., Daudi, Arsalan, Petzold, Christopher J., Singan, Vasanth R., Kuo, Rita, Chovatia, Mansi, Daum, Christopher, Heazlewood, Joshua L., Zipfel, Cyril, and Ronald, Pamela C.. Mon . "Transgenic expression of the dicotyledonous pattern recognition receptor EFR in rice leads to ligand-dependent activation of defense responses". United States. doi:10.1371/journal.ppat.1004809. https://www.osti.gov/servlets/purl/1191186.
@article{osti_1191186,
title = {Transgenic expression of the dicotyledonous pattern recognition receptor EFR in rice leads to ligand-dependent activation of defense responses},
author = {Schwessinger, Benjamin and Bahar, Ofir and Thomas, Nicolas and Holton, Nicolas and Nekrasov, Vladimir and Ruan, Deling and Canlas, Patrick E. and Daudi, Arsalan and Petzold, Christopher J. and Singan, Vasanth R. and Kuo, Rita and Chovatia, Mansi and Daum, Christopher and Heazlewood, Joshua L. and Zipfel, Cyril and Ronald, Pamela C.},
abstractNote = {Plant plasma membrane localized pattern recognition receptors (PRRs) detect extracellular pathogen-associated molecules. PRRs such as Arabidopsis EFR and rice XA21 are taxonomically restricted and are absent from most plant genomes. Here we show that rice plants expressing EFR or the chimeric receptor EFR::XA21, containing the EFR ectodomain and the XA21 intracellular domain, sense both Escherichia coli- and Xanthomonas oryzae pv. oryzae (Xoo)-derived elf18 peptides at sub-nanomolar concentrations. Treatment of EFR and EFR::XA21 rice leaf tissue with elf18 leads to MAP kinase activation, reactive oxygen production and defense gene expression. Although expression of EFR does not lead to robust enhanced resistance to fully virulent Xoo isolates, it does lead to quantitatively enhanced resistance to weakly virulent Xoo isolates. EFR interacts with OsSERK2 and the XA21 binding protein 24 (XB24), two key components of the rice XA21-mediated immune response. Rice-EFR plants silenced for OsSERK2, or overexpressing rice XB24 are compromised in elf18-induced reactive oxygen production and defense gene expression indicating that these proteins are also important for EFR-mediated signaling in transgenic rice. Taken together, our results demonstrate the potential feasibility of enhancing disease resistance in rice and possibly other monocotyledonous crop species by expression of dicotyledonous PRRs. Our results also suggest that Arabidopsis EFR utilizes at least a subset of the known endogenous rice XA21 signaling components.},
doi = {10.1371/journal.ppat.1004809},
journal = {PLoS Pathogens},
number = 3,
volume = 11,
place = {United States},
year = {Mon Mar 30 00:00:00 EDT 2015},
month = {Mon Mar 30 00:00:00 EDT 2015}
}

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